Q2RNG5 · BLUB_RHORT
- Protein5,6-dimethylbenzimidazole synthase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids211 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Involved in the biosynthesis of cobalamin (vitamin B12). Catalyzes the oxidative fragmentation and contraction of the isoalloxazine heterocycle and the cleavage of the ribityl tail of FMNH2 to form 5,6-dimethylbenzimidazole (DMB) and D-erythrose 4-phosphate (E4P). NAD(P)H is only required initially to reduce FMN and oxygen drives the oxidative fragmentation.
Catalytic activity
- FMNH2 + O2 = 5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + dialurate + H+
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 5,6-dimethylbenzimidazole synthase activity | |
Molecular Function | nucleotide binding | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | cobalamin biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5,6-dimethylbenzimidazole synthase
- EC number
- Short namesDMB synthase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rhodospirillales > Rhodospirillaceae > Rhodospirillum
Accessions
- Primary accessionQ2RNG5
Proteomes
Phenotypes & Variants
Disruption phenotype
Cells lacking this gene fail to synthesize cobalamin.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 22 | Completely abolishes DMB formation. | ||||
Sequence: R → E |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000424084 | 1-211 | 5,6-dimethylbenzimidazole synthase | |||
Sequence: MRTGPLFDPSFRDGLDALFQWRRDVRHFRKDPIDEETVARLLACADLAPSVGNSQPWRFVRVDDGARRGVIIDDFTRCNAAARALQPEERQDAYARLKLEGLREAPLQLAVFCDEATDQGHGLGQATMPETRRYSVVMAIHTLWLAARARGLGVGWVSVLDPQTVTAALDVPAEWAFVAYLCIGWPREEHPIPELERLGWQSRRPHPVVRR |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length211
- Mass (Da)23,904
- Last updated2006-01-24 v1
- Checksum1687F48488019840
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000230 EMBL· GenBank· DDBJ | ABC24330.1 EMBL· GenBank· DDBJ | Genomic DNA |