Q2QAV0 · TIO_ARATH

Function

function

Plays a role in conventional modes of cytokinesis in meristems and during male gametogenesis but also acts in nonconventional modes of cytokinesis (cellularization) during female gametogenesis. Constitutes a signaling module in association with Kinesin-12 members that is required to support phragmoplast expansion and cell-plate growth in plant cells.

Catalytic activity

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site12-20ATP (UniProtKB | ChEBI)
Binding site35ATP (UniProtKB | ChEBI)
Active site127Proton acceptor

GO annotations

AspectTerm
Cellular Componentcytoskeleton
Cellular Componentphragmoplast
Molecular FunctionATP binding
Molecular Functionkinesin binding
Molecular Functionprotein serine kinase activity
Molecular Functionprotein serine/threonine kinase activity
Biological Processcytokinesis by cell plate formation
Biological Processembryo sac cellularization
Biological Processmale meiosis cytokinesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Serine/threonine-protein kinase TIO
  • EC number
  • Alternative names
    • Fused homolog (AtFUSED)
    • Protein TWO-IN-ONE (AtTIO)

Gene names

    • Name
      TIO
    • Synonyms
      FU
    • ORF names
      F14I3.15
    • Ordered locus names
      At1g50240

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q2QAV0
  • Secondary accessions
    • Q2QB51
    • Q9SX42

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Note: Localized to the midline of the nascent phragmoplast and remains associated with the expanding phragmoplast ring.

Keywords

Phenotypes & Variants

Disruption phenotype

Cytokinesis-defective leading to aberrant pollen and embryo sacs.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis35Alters binding to Kinesin-12 members.
Mutagenesis127Alters binding to Kinesin-12 members; when associated with A-132.
Mutagenesis132Alters binding to Kinesin-12 members; when associated with A-127.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 72 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004196941-1322Serine/threonine-protein kinase TIO

Proteomic databases

PTM databases

Expression

Tissue specificity

Ubiquitous.

Gene expression databases

Interaction

Subunit

Interacts with Kinesin-12 members KIN12A/PAKRP1 and KIN12B/PAKRP1L (PubMed:22709276).
Interacts with KIN7B/NACK2 (PubMed:24146312).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q2QAV0KIN12A Q9LDN03EBI-6280536, EBI-6280428

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region, repeat.

TypeIDPosition(s)Description
Domain6-256Protein kinase
Region1000-1322Required for the binding to Kinesin-12 members
Repeat1056-1098ARM 1
Repeat1101-1140ARM 2
Repeat1143-1182ARM 3
Repeat1183-1223ARM 4
Repeat1226-1273ARM 5
Repeat1281-1320ARM 6

Domain

The ARM-repeat containing C-terminal region is required for the binding to the Kinesin-12 members.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,322
  • Mass (Da)
    145,414
  • Last updated
    2006-01-24 v1
  • Checksum
    EBAB948B8406BCCC
MGVEDYHVIELVGEGSFGRVYKGRRKYTGQTVAMKFIMKQGKTDKDIHSLRQEIEILRKLKHENIIEMLDSFENAREFCVVTEFAQGELFEILEDDKCLPEEQVQAIAKQLVKALDYLHSNRIIHRDMKPQNILIGAGSVVKLCDFGFARAMSTNTVVLRSIKGTPLYMAPELVKEQPYDRTVDLWSLGVILYELYVGQPPFYTNSVYALIRHIVKDPVKYPDEMSTYFESFLKGLLNKEPHSRLTWPALREHPFVKETQEEVEAREIHTAVVDNKAAWMLKGNGGQQRNEKCDSVTLVEDMSATKGLADVQSDMKSAVKVNSPPTEDFVGFPTQEEIKSSGNPTLDKLENTSRTVKGAQVIGENDKALDLVLLSLERFSKSPDSKRDKDVACSVQSLRIISNLVATRAIVSVGLIEKITCALLDFTDALVGMKSPEFNNIIPKSLSVTKNLVGHVEGNNIHSSYIRHWTKVVEIFIQVVRWEEEGTGRIIYEACSCITTMLSRVAQDLKSSTPDSVSKQILEHANMSRIVDHLCLCLASSGSSLTSGSSQMLAAACEACRAIWILIDTSETFFKNDDVNILPLDALQNRLSQHDIGNSEWGPLSEKLVDTVTRAYLRSKHVQVAVGHCLHQRVEAPLVSAIQLLSRCCLHNGILPSMLCGLPSSLPITTVVSGGEDGTVISEIFSILSYATLSSKDQQTGEKDNFEGRLNNLVFHSCLMLATVAQCLKLTGRNSVLLMLTTSPKKHQHRLSAIANHIASDDKIEASLQNHSASAMLALASILALEKGSSAGSSVSELVVSLIPRATKLCYHLRPMPSNEGEVISHSANYAKWHGLLDGCIGLLESRLKWGGPLAVQQLIASGTPLLLINLLAGKLSNASPEDIKKTSNRIGLSPIGVVWTISSICHCLSGGTTFRQVLVKIETMKLITCLLSDAHIKLVKSWGGPGGGKDGVRETINVIIDLLAFPFVALQSQPGSLSATASVNSGFILNIGSPGVRVCMEDRDLLKAIEEDMDKYIIVLLEVGVPSLILRCLDHLELKDLVRPVAFLAKMVGRPRLAVDLVSKGLLDPNRMKKLLNQSSPREVILDILMIISDLSRMDKAFYKYIGEASVLQPLKEYLTHVDPNIRAKACSALGNMCRHNGYFYSALAEHQIIGLLIDRCADPDKRTQKFACFAIGNAAYHNDTLYEELRRSITQLANVLTTAEEDKTKANAAGALSNLVRNSNKLCEDIVSKGALQTLLRLVADCSTLALNPSKKETASESPLKIALFSLAKMCSNHQICRQFVKSSELFPVIARLKQSPEANIAHYASVIVAKVSGES

Sequence caution

The sequence AAD50043.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict230in Ref. 1; AAZ66047

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DQ149983
EMBL· GenBank· DDBJ
AAZ66047.1
EMBL· GenBank· DDBJ
mRNA
DQ153170
EMBL· GenBank· DDBJ
AAZ66048.1
EMBL· GenBank· DDBJ
Genomic DNA
AC007980
EMBL· GenBank· DDBJ
AAD50043.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002684
EMBL· GenBank· DDBJ
AEE32527.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
ANM58830.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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