Q2QAV0 · TIO_ARATH
- ProteinSerine/threonine-protein kinase TIO
- GeneTIO
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1322 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays a role in conventional modes of cytokinesis in meristems and during male gametogenesis but also acts in nonconventional modes of cytokinesis (cellularization) during female gametogenesis. Constitutes a signaling module in association with Kinesin-12 members that is required to support phragmoplast expansion and cell-plate growth in plant cells.
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoskeleton | |
Cellular Component | phragmoplast | |
Molecular Function | ATP binding | |
Molecular Function | kinesin binding | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Biological Process | cytokinesis by cell plate formation | |
Biological Process | embryo sac cellularization | |
Biological Process | male meiosis cytokinesis |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSerine/threonine-protein kinase TIO
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ2QAV0
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localized to the midline of the nascent phragmoplast and remains associated with the expanding phragmoplast ring.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Cytokinesis-defective leading to aberrant pollen and embryo sacs.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 35 | Alters binding to Kinesin-12 members. | ||||
Sequence: K → A | ||||||
Mutagenesis | 127 | Alters binding to Kinesin-12 members; when associated with A-132. | ||||
Sequence: D → A | ||||||
Mutagenesis | 132 | Alters binding to Kinesin-12 members; when associated with A-127. | ||||
Sequence: N → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 72 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000419694 | 1-1322 | Serine/threonine-protein kinase TIO | |||
Sequence: MGVEDYHVIELVGEGSFGRVYKGRRKYTGQTVAMKFIMKQGKTDKDIHSLRQEIEILRKLKHENIIEMLDSFENAREFCVVTEFAQGELFEILEDDKCLPEEQVQAIAKQLVKALDYLHSNRIIHRDMKPQNILIGAGSVVKLCDFGFARAMSTNTVVLRSIKGTPLYMAPELVKEQPYDRTVDLWSLGVILYELYVGQPPFYTNSVYALIRHIVKDPVKYPDEMSTYFESFLKGLLNKEPHSRLTWPALREHPFVKETQEEVEAREIHTAVVDNKAAWMLKGNGGQQRNEKCDSVTLVEDMSATKGLADVQSDMKSAVKVNSPPTEDFVGFPTQEEIKSSGNPTLDKLENTSRTVKGAQVIGENDKALDLVLLSLERFSKSPDSKRDKDVACSVQSLRIISNLVATRAIVSVGLIEKITCALLDFTDALVGMKSPEFNNIIPKSLSVTKNLVGHVEGNNIHSSYIRHWTKVVEIFIQVVRWEEEGTGRIIYEACSCITTMLSRVAQDLKSSTPDSVSKQILEHANMSRIVDHLCLCLASSGSSLTSGSSQMLAAACEACRAIWILIDTSETFFKNDDVNILPLDALQNRLSQHDIGNSEWGPLSEKLVDTVTRAYLRSKHVQVAVGHCLHQRVEAPLVSAIQLLSRCCLHNGILPSMLCGLPSSLPITTVVSGGEDGTVISEIFSILSYATLSSKDQQTGEKDNFEGRLNNLVFHSCLMLATVAQCLKLTGRNSVLLMLTTSPKKHQHRLSAIANHIASDDKIEASLQNHSASAMLALASILALEKGSSAGSSVSELVVSLIPRATKLCYHLRPMPSNEGEVISHSANYAKWHGLLDGCIGLLESRLKWGGPLAVQQLIASGTPLLLINLLAGKLSNASPEDIKKTSNRIGLSPIGVVWTISSICHCLSGGTTFRQVLVKIETMKLITCLLSDAHIKLVKSWGGPGGGKDGVRETINVIIDLLAFPFVALQSQPGSLSATASVNSGFILNIGSPGVRVCMEDRDLLKAIEEDMDKYIIVLLEVGVPSLILRCLDHLELKDLVRPVAFLAKMVGRPRLAVDLVSKGLLDPNRMKKLLNQSSPREVILDILMIISDLSRMDKAFYKYIGEASVLQPLKEYLTHVDPNIRAKACSALGNMCRHNGYFYSALAEHQIIGLLIDRCADPDKRTQKFACFAIGNAAYHNDTLYEELRRSITQLANVLTTAEEDKTKANAAGALSNLVRNSNKLCEDIVSKGALQTLLRLVADCSTLALNPSKKETASESPLKIALFSLAKMCSNHQICRQFVKSSELFPVIARLKQSPEANIAHYASVIVAKVSGES |
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Interacts with Kinesin-12 members KIN12A/PAKRP1 and KIN12B/PAKRP1L (PubMed:22709276).
Interacts with KIN7B/NACK2 (PubMed:24146312).
Interacts with KIN7B/NACK2 (PubMed:24146312).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q2QAV0 | KIN12A Q9LDN0 | 3 | EBI-6280536, EBI-6280428 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 6-256 | Protein kinase | ||||
Sequence: YHVIELVGEGSFGRVYKGRRKYTGQTVAMKFIMKQGKTDKDIHSLRQEIEILRKLKHENIIEMLDSFENAREFCVVTEFAQGELFEILEDDKCLPEEQVQAIAKQLVKALDYLHSNRIIHRDMKPQNILIGAGSVVKLCDFGFARAMSTNTVVLRSIKGTPLYMAPELVKEQPYDRTVDLWSLGVILYELYVGQPPFYTNSVYALIRHIVKDPVKYPDEMSTYFESFLKGLLNKEPHSRLTWPALREHPFV | ||||||
Region | 1000-1322 | Required for the binding to Kinesin-12 members | ||||
Sequence: CMEDRDLLKAIEEDMDKYIIVLLEVGVPSLILRCLDHLELKDLVRPVAFLAKMVGRPRLAVDLVSKGLLDPNRMKKLLNQSSPREVILDILMIISDLSRMDKAFYKYIGEASVLQPLKEYLTHVDPNIRAKACSALGNMCRHNGYFYSALAEHQIIGLLIDRCADPDKRTQKFACFAIGNAAYHNDTLYEELRRSITQLANVLTTAEEDKTKANAAGALSNLVRNSNKLCEDIVSKGALQTLLRLVADCSTLALNPSKKETASESPLKIALFSLAKMCSNHQICRQFVKSSELFPVIARLKQSPEANIAHYASVIVAKVSGES | ||||||
Repeat | 1056-1098 | ARM 1 | ||||
Sequence: PRLAVDLVSKGLLDPNRMKKLLNQSSPREVILDILMIISDLSR | ||||||
Repeat | 1101-1140 | ARM 2 | ||||
Sequence: KAFYKYIGEASVLQPLKEYLTHVDPNIRAKACSALGNMCR | ||||||
Repeat | 1143-1182 | ARM 3 | ||||
Sequence: GYFYSALAEHQIIGLLIDRCADPDKRTQKFACFAIGNAAY | ||||||
Repeat | 1183-1223 | ARM 4 | ||||
Sequence: HNDTLYEELRRSITQLANVLTTAEEDKTKANAAGALSNLVR | ||||||
Repeat | 1226-1273 | ARM 5 | ||||
Sequence: NKLCEDIVSKGALQTLLRLVADCSTLALNPSKKETASESPLKIALFSL | ||||||
Repeat | 1281-1320 | ARM 6 | ||||
Sequence: QICRQFVKSSELFPVIARLKQSPEANIAHYASVIVAKVSG |
Domain
The ARM-repeat containing C-terminal region is required for the binding to the Kinesin-12 members.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,322
- Mass (Da)145,414
- Last updated2006-01-24 v1
- ChecksumEBAB948B8406BCCC
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 230 | in Ref. 1; AAZ66047 | ||||
Sequence: E → K |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ149983 EMBL· GenBank· DDBJ | AAZ66047.1 EMBL· GenBank· DDBJ | mRNA | ||
DQ153170 EMBL· GenBank· DDBJ | AAZ66048.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AC007980 EMBL· GenBank· DDBJ | AAD50043.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002684 EMBL· GenBank· DDBJ | AEE32527.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | ANM58830.1 EMBL· GenBank· DDBJ | Genomic DNA |