Q2N950 · MIAB_ERYLH
- ProteintRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
- GenemiaB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids446 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.
Catalytic activity
- N6-dimethylallyladenosine37 in tRNA + [sulfur carrier]-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N6-dimethylallyladenosine37 in tRNA + [sulfur carrier]-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H+
Cofactor
Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 17 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 53 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 87 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 160 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 164 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 167 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | methylthiotransferase activity | |
Biological Process | tRNA methylthiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Erythrobacteraceae > Erythrobacter/Porphyrobacter group > Erythrobacter
Accessions
- Primary accessionQ2N950
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000374280 | 1-446 | tRNA-2-methylthio-N6-dimethylallyladenosine synthase | |||
Sequence: MKPTHSPKTYRVKSFGCQMNVYDGERMAEMLDEKGIEPAPEGEDADLVVLNTCHIREKAVDKVYSDIGRLTKGKTQTKAPMIAVAGCVAQAEGEEIMARAPAVSMVVGPQAYHRLPGMIDAAVAGKRSTDTDMPADAKFAALPKRRKSAPSAFLTIQEGCDKFCTYCVVPYTRGAEISRPFSALIDEAKKLVEAGAKEITLLGQNVSAWTGEDAKGRALGMAGLIRELAKDPDLKRVRYTTSHPADMDDELIATHGEVEKLMPYLHLPVQSGNDRVLKAMNRSHTAESYLRLLERFRAARPDLALSGDFIVGFPGETEAEFEDTLKIVDEVRYAQAYSFKYSPRPGTPAATMERQVPKEVMDERLQRLQAALNRDQAAFNAGSVGRTCEVLVERTGKHPGQWLGKSPWLQSVWFDGDVAIGDLVQVELVEAGPNSLAGQLLETVAA |
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 8-124 | MTTase N-terminal | ||||
Sequence: KTYRVKSFGCQMNVYDGERMAEMLDEKGIEPAPEGEDADLVVLNTCHIREKAVDKVYSDIGRLTKGKTQTKAPMIAVAGCVAQAEGEEIMARAPAVSMVVGPQAYHRLPGMIDAAVA | ||||||
Domain | 146-378 | Radical SAM core | ||||
Sequence: RKSAPSAFLTIQEGCDKFCTYCVVPYTRGAEISRPFSALIDEAKKLVEAGAKEITLLGQNVSAWTGEDAKGRALGMAGLIRELAKDPDLKRVRYTTSHPADMDDELIATHGEVEKLMPYLHLPVQSGNDRVLKAMNRSHTAESYLRLLERFRAARPDLALSGDFIVGFPGETEAEFEDTLKIVDEVRYAQAYSFKYSPRPGTPAATMERQVPKEVMDERLQRLQAALNRDQAA | ||||||
Domain | 381-442 | TRAM | ||||
Sequence: AGSVGRTCEVLVERTGKHPGQWLGKSPWLQSVWFDGDVAIGDLVQVELVEAGPNSLAGQLLE |
Sequence similarities
Belongs to the methylthiotransferase family. MiaB subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length446
- Mass (Da)48,782
- Last updated2006-02-07 v1
- Checksum5DCA58C06405733B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000157 EMBL· GenBank· DDBJ | ABC63791.1 EMBL· GenBank· DDBJ | Genomic DNA |