Q2N695 · SUCC_ERYLH
- ProteinSuccinate--CoA ligase [ADP-forming] subunit beta
- GenesucC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids399 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Catalytic activity
- succinate + ATP + CoA = succinyl-CoA + ADP + phosphateThis reaction proceeds in the backward direction.
- GTP + succinate + CoA = succinyl-CoA + GDP + phosphateThis reaction proceeds in the backward direction.
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 46 | ATP (UniProtKB | ChEBI) | |||
Binding site | 53-55 | ATP (UniProtKB | ChEBI) | |||
Binding site | 112 | ATP (UniProtKB | ChEBI) | |||
Binding site | 117 | ATP (UniProtKB | ChEBI) | |||
Binding site | 209 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 223 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 274 | substrate; ligand shared with subunit alpha | |||
Binding site | 331-333 | substrate; ligand shared with subunit alpha | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | succinate-CoA ligase complex | |
Molecular Function | ATP binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | succinate-CoA ligase (ADP-forming) activity | |
Molecular Function | succinate-CoA ligase (GDP-forming) activity | |
Biological Process | succinyl-CoA metabolic process | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSuccinate--CoA ligase [ADP-forming] subunit beta
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Erythrobacteraceae > Erythrobacter/Porphyrobacter group > Erythrobacter
Accessions
- Primary accessionQ2N695
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000082083 | 1-399 | Succinate--CoA ligase [ADP-forming] subunit beta | ||
Interaction
Subunit
Heterotetramer of two alpha and two beta subunits.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 9-254 | ATP-grasp | |||
Sequence similarities
Belongs to the succinate/malate CoA ligase beta subunit family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length399
- Mass (Da)42,505
- Last updated2006-02-07 v1
- ChecksumB0863FABF3D57293
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000157 EMBL· GenBank· DDBJ | ABC64796.1 EMBL· GenBank· DDBJ | Genomic DNA |