Q2N5Q5 · RPOC_ERYLH
- ProteinDNA-directed RNA polymerase subunit beta'
- GenerpoC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1438 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 2 Zn2+ ions per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 70 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 72 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 85 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 88 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 461 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 463 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 465 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 821 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 895 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 902 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 905 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta'
- EC number
- Short namesRNAP subunit beta'
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Erythrobacteraceae > Erythrobacter/Porphyrobacter group > Erythrobacter
Accessions
- Primary accessionQ2N5Q5
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000353354 | 1-1438 | DNA-directed RNA polymerase subunit beta' | |||
Sequence: MNELTKFTNQLAKPETFDQIQIGIASPERIRSWSFGEIKKPETINYRTFKPERDGLFCARIFGPVKDYECLCGKYKRMKYKGVVCEKCGVEVTVTKVRRERMGHIELAAPVAHIWFLKSLPSRIGLLLDMQLKQLERVLYFEHYIVTEPGLTPLEKFQLLTEDELLEAQDEYGEDAFSAGIGAEAVKFMLMDLDLEQEKEDLLEELATTKSKLKPAKIIKRLKVVESFIESGNRPEWMILEVVPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLIELRAPDIIVRNEKRMLQESVDALFDNGRRGRVITGANKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVTGPELKLHQCGLPKKMALELFKPFIYARLDAKGLSMTLKQAKKWVEKERKEVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCSAFNADFDGDQMAVHVPLSLEAQLEARVLMMSTNNILSPANGKPIIVPSQDMVLGLYYLSMERQEKTPEYIEEKDGTKIEKLPRFADMAEVHQALETKSVTLHTRIIARVPQADEDGKPEMKRFVTTPGRMLIGECLPKNHKVPYDIVNRLLTKKEIGDVIDQVYRHTGQKDTVLFADAIMVLGFRHAFKAGISFGKDDMIIPDSKEGMIEDTKKQVADYEQQYQDGLITQQEKYNKVIDAWSRCGDQVAEAMMDEIKSQKFDKDGKESEINSIYMMSHSGARGSPAQMKQLAGMRGLMAKPSGEIIENPIISNFKEGLTVLEYFNSTHGARKGLADTALKTANSGYLTRRLVDVSQDCVIVEEDCKTDNALEMRAIVQGGSVIASLGERILGRTLVDDLVNAKTDEVIVKAGTLLDEPMVKAIEEAEVQVARIRSPLVCEADQGVCATCYGRDLARGTPVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGAAQLNETSHLESISDGKVEYRDMPTITDKKGRILSLARNGELAVIDAEGREREIHKVPYGTVLMHKDGAKVKEGDRLAEWDPFSLPIITEQSGVVKYQDLIEGTTLEEQTDDATGIAQRVVTENRATGRKKKEDLRPRLTLLSEGQSEDETEAQRYLLAPGTTLSVTDGQTVEAGDILARASREAAKTRDITGGLPRVAELFEARVPKDNAVIAKISGKIEFVREYKAKRKIAIVPEEGEAVDYLIPKTKVIDVQEGDFVKKGDTLISGSPNPHDILDVLGVEALAEYLCTEIQEVYRLQGVKINDKHIEVIVRQMLQKVEITDGGDTVLLPGEQVDRDEMDEANAKLGRGKKPATGNPVLLGITKASLQTRSFISAASFQETTRVLTQASVEGKKDTLIGLKENVIVGRLIPAGTGAGMNRMRVTASARDAALRAQWKKQQEKLAAADEAAMAAEKEPEAEVMDADAMAAAMGGDSAGGDTKPEAPEASEE |
Interaction
Subunit
The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1413-1438 | Disordered | ||||
Sequence: DAMAAAMGGDSAGGDTKPEAPEASEE |
Sequence similarities
Belongs to the RNA polymerase beta' chain family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,438
- Mass (Da)159,738
- Last updated2006-02-07 v1
- ChecksumE6251C98C4E68B5C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000157 EMBL· GenBank· DDBJ | ABC64986.1 EMBL· GenBank· DDBJ | Genomic DNA |