Q2MFP3 · DOIAD_STREY
- Protein2-deoxy-scyllo-inosamine dehydrogenase
- GeneparE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids339 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD+ or NADP+, forming 3-amino-2,3-dideoxy-scyllo-inosose (amino-DOI).
Catalytic activity
- 2-deoxy-scyllo-inosamine + NADP+ = 3-amino-2,3-dideoxy-scyllo-inosose + NADPH + H+
Cofactor
Note: Binds 2 Zn2+ ions per subunit.
Pathway
Metabolic intermediate biosynthesis; 2-deoxystreptamine biosynthesis; 2-deoxystreptamine from D-glucose 6-phosphate: step 3/4.
Antibiotic biosynthesis; paromomycin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 37 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: C | ||||||
Binding site | 59 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: H | ||||||
Binding site | 88 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 91 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 94 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 102 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 143 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | oxidoreductase activity | |
Molecular Function | zinc ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-deoxy-scyllo-inosamine dehydrogenase
- EC number
- Short namesDOIA dehydrogenase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces
Accessions
- Primary accessionQ2MFP3
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000234047 | 1-339 | 2-deoxy-scyllo-inosamine dehydrogenase | |||
Sequence: MKALMFKAPLQAVLTERDVPEPAPGEALVKLAYNSICGSDLSFYKGVWHGFTYPVVPGHEWSGTVVEAGGGAADLVGQNVVGDLTVACGSCRHCTVGKPTLCADLQELGFTRDGACAEYMTVPTGNLHRLPEGLSLREATQVEPLAVALNAVDRLAVTAGEKVAITGAGGIGLLLAQAVRLRGAEVTVLAEPVTERRQAAHALGVPHTVGGDPGELVGFVEKHPELTPDVVLEASGYPLAVQEAVEAVRSGGRIGLIGYRIEEAATMAPHHIVVKVLSLQASMGPGDRFGEAIELLAAGAVDVDALLSHEFGLADHDRALDVALRRADGNTRSYFNLRA |
Structure
Family & Domains
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length339
- Mass (Da)35,458
- Last updated2006-02-21 v1
- Checksum76E63F9530E1859D