Q2M405 · CRN8_PHYIN
- ProteinCrinkler effector protein 8
- GeneCRN8
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids599 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Secreted effector that induces cell death when expressed in host plants (PubMed:19741609, PubMed:20847293, PubMed:22927814).
Acts as a kinase and is able to autophosphorylate, however its cell death inducing ability is not a direct result of its kinase activity, but rather a consequence of the phosphorylated state of the five identified serine residues in the CRN8 protein (PubMed:22927814).
Acts as a kinase and is able to autophosphorylate, however its cell death inducing ability is not a direct result of its kinase activity, but rather a consequence of the phosphorylated state of the five identified serine residues in the CRN8 protein (PubMed:22927814).
Catalytic activity
- L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H+
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 470 | Proton acceptor | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | host cell nucleus | |
Molecular Function | histone H2AS1 kinase activity | |
Molecular Function | protein serine kinase activity | |
Biological Process | phosphorylation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCrinkler effector protein 8
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Sar > Stramenopiles > Oomycota > Peronosporales > Peronosporaceae > Phytophthora
Accessions
- Primary accessionQ2M405
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 249 | Impairs host cell death induction; when associated with A-281, A-385, A-474 and A-587. | |||
Mutagenesis | 281 | Impairs host cell death induction; when associated with A-249, A-385, A-474 and A-587. | |||
Mutagenesis | 385 | Impairs host cell death induction; when associated with A-249, A-281, A-474 and A-587. | |||
Mutagenesis | 469 | Destabilizes the CRN8 protein and impairs autophosphorylation and host cell death induction; when associated with A-470. | |||
Mutagenesis | 470 | Destabilizes the CRN8 protein and impairs autophosphorylation and host cell death induction; when associated with A-469. | |||
Mutagenesis | 470 | Impairs autophosphorylation but does not affect host cell death induction. | |||
Mutagenesis | 474 | Impairs host cell death induction; when associated with A-249, A-291, A-385 and A-587. | |||
Mutagenesis | 587 | Impairs host cell death induction; when associated with A-249, A-291, A-385 and A-474. | |||
PTM/Processing
Features
Showing features for signal, chain, glycosylation, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-17 | ||||
Chain | PRO_0000447409 | 18-599 | Crinkler effector protein 8 | ||
Glycosylation | 23 | N-linked (GlcNAc...) asparagine | |||
Modified residue | 249 | Phosphoserine | |||
Modified residue | 281 | Phosphoserine | |||
Modified residue | 385 | Phosphoserine | |||
Modified residue | 474 | Phosphoserine | |||
Modified residue | 587 | Phosphoserine | |||
Post-translational modification
Autophosphorylated at Ser-249, Ser-281, Ser-385, Ser-474 and Ser-587. Additional serines or threonines are also targeted for phosphorylation.
Keywords
- PTM
PTM databases
Interaction
Subunit
Dimerizes in host plants.
Structure
Family & Domains
Features
Showing features for region, motif, domain, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 18-52 | LQLFLAK domain | |||
Region | 53-109 | DWL domain | |||
Motif | 110-116 | HVLVXXP motif | |||
Region | 117-590 | C-terminal D2 effector domain | |||
Domain | 289-590 | Protein kinase | |||
Compositional bias | 577-591 | Basic and acidic residues | |||
Region | 577-599 | Disordered | |||
Motif | 590-599 | Host nuclear localization signal | |||
Domain
The CRN proteins have modular architectures that include a signal peptide, a conserved N-terminus, and highly diverse C-terminal domains. The conserved CRN N-terminus harbors a distinct LXLFLAK motif, which is followed by the conserved DWL domain. A highly conserved HVLVXXP motif marks the end of the CRN N-terminal domains and forms a junction where diverse C-terminal effector domains are fused. The conserved CRN N-terminus mediates the translocation into the plant host cells.
The whole D2 effector domain that contains a protein functional kinase domain, as well as the C-terminal NLS are required for cell death induction.
Sequence similarities
In the N-terminal section; belongs to the Crinkler effector family.
In the C-terminal section; belongs to the protein kinase superfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length599
- Mass (Da)67,268
- Last updated2006-02-21 v1
- Checksum5258452242BA845E
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 577-591 | Basic and acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY961456 EMBL· GenBank· DDBJ | AAY43402.1 EMBL· GenBank· DDBJ | mRNA |