Q2LMP1 · WNT3A_CHICK

Function

function

Isoform 1

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:17488271).
Regulates chick apical ectodermal ridge formation (PubMed:17488271).
Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity).

Caution

A palmitoylation site was proposed at Cys-77, but it was later shown that this cysteine is engaged in a disulfide bond.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytosol
Cellular Componentendoplasmic reticulum
Cellular Componentextracellular space
Cellular Componentnuclear outer membrane
Cellular Componentplasma membrane
Molecular Functioncytokine activity
Molecular Functionfrizzled binding
Biological ProcessBMP signaling pathway
Biological Processcanonical Wnt signaling pathway
Biological Processcell fate commitment
Biological Processcornea development in camera-type eye
Biological Processdetermination of dorsal identity
Biological Processdorsal/ventral pattern formation
Biological Processinner ear morphogenesis
Biological Processlimb development
Biological Processlimb morphogenesis
Biological Processneuron differentiation
Biological Processpositive regulation of canonical Wnt signaling pathway
Biological Processpositive regulation of epithelial cell proliferation
Biological Processpositive regulation of fibroblast growth factor receptor signaling pathway
Biological Processpositive regulation of gene expression
Biological Processpositive regulation of myoblast differentiation
Biological Processpositive regulation of myotube differentiation
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processregulation of canonical Wnt signaling pathway
Biological Processregulation of fibroblast growth factor receptor signaling pathway

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein Wnt-3a

Gene names

    • Name
      WNT3A

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Galloanserae > Galliformes > Phasianidae > Phasianinae > Gallus

Accessions

  • Primary accession
    Q2LMP1
  • Secondary accessions
    • Q9PWH1

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for signal, chain, disulfide bond, glycosylation, lipidation.

Type
IDPosition(s)Description
Signal1-18
ChainPRO_000039292419-352Protein Wnt-3a
Disulfide bond77↔88
Glycosylation87N-linked (GlcNAc...) asparagine
Glycosylation92N-linked (GlcNAc...) asparagine
Disulfide bond128↔136
Disulfide bond138↔155
Disulfide bond203↔217
Disulfide bond205↔212
Lipidation209O-palmitoleoyl serine; by PORCN
Disulfide bond281↔312
Disulfide bond297↔307
Glycosylation298N-linked (GlcNAc...) asparagine
Disulfide bond311↔351
Disulfide bond327↔342
Disulfide bond329↔339
Disulfide bond334↔335

Post-translational modification

Palmitoleoylation is required for efficient binding to frizzled receptors (PubMed:22046319).
Depalmitoleoylation leads to inhibit the Wnt signaling pathway (By similarity).
Disulfide bonds have critical and distinct roles in secretion and activity. Loss of each conserved cysteine in WNT3A results in high molecular weight oxidized Wnt oligomers, which are formed through inter-Wnt disulfide bonding (By similarity).

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in cornea. Isoform 1 is expressed in the primitive streak, dorsal neural tube, proximal otic vesicle, the apical ectodermal ridge and the epithelium of feather buds.

Developmental stage

In the eye, the expression was observed at stage 23 in the ocular surface epithelium ventral to the corneal epithelium. Strongly expressed in the entire ocular surface at stage 26 including the corneal epithelium. Remained strongly expressed at stage 30.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 2 isoforms produced by Alternative splicing.

Q2LMP1-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    352
  • Mass (Da)
    39,629
  • Last updated
    2006-02-21 v1
  • Checksum
    EE558AC4B2225B3A
MASFGYFLFLCGLSQALSSYPIWWSLAIGHQYSSLGTQPILCGSIPGLVPKQLRFCRNYVEIMPSVAEGVKIGIQECQHQFRGRRWNCTTVNDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGSATICGCDTRHKGSPGEGWKWGGCSEDVEFGSMVSREFADARENRPDARSAMNRHNNEAGRTSIIELMHLKCKCHGLSGSCEVKTCWWSQPDFRVIGDYLKDKYDSASEMVVEKHRESRGWVETLRPKYNFFKAPTEKDLVYYENSPNFCEPNPETGSFGTRDRICNVTSHGIDGCDLLCCGRGHNTRTEKRKEKCHCIFHWCCYVRCQECIRVYDVHTCK

Q2LMP1-2

  • Name
    2
  • Note
    Not secreted.
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-24: MASFGYFLFLCGLSQALSSYPIWW → MKSFCSEVVAKSRLGLKQWGWCGWTPMGSAWKKWISEQRSSLELWDVG

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8V1A0A9A0A8V1A0A9_CHICKWNT3A356
A0A8V1A7U9A0A8V1A7U9_CHICKWNT3A333
A0A8V1A7V3A0A8V1A7V3_CHICKWNT3A376
A0A8V1ACU2A0A8V1ACU2_CHICKWNT3A334

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0388511-24in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB024080
EMBL· GenBank· DDBJ
BAA83743.1
EMBL· GenBank· DDBJ
mRNA
DQ022307
EMBL· GenBank· DDBJ
AAY87456.1
EMBL· GenBank· DDBJ
mRNA
EF068232
EMBL· GenBank· DDBJ
ABK90821.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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