Q2KNB9 · HXK2_ORYSJ
- ProteinHexokinase-2
- GeneHXK2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids494 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Fructose and glucose phosphorylating enzyme.
Catalytic activity
- a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H+This reaction proceeds in the forward direction.
Pathway
Carbohydrate metabolism; hexose metabolism.
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 101 | ADP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 102 | ADP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 103 | ADP (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 191 | D-glucose (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 192 | D-glucose (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 226 | D-glucose (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 227 | D-glucose (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 250 | ADP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 253 | D-glucose (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 281 | D-glucose (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 312 | D-glucose (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 437 | ADP (UniProtKB | ChEBI) | ||||
Sequence: G |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Molecular Function | ATP binding | |
Molecular Function | D-glucose binding | |
Molecular Function | fructokinase activity | |
Molecular Function | glucokinase activity | |
Biological Process | carbohydrate phosphorylation | |
Biological Process | glucose 6-phosphate metabolic process | |
Biological Process | glucose metabolic process | |
Biological Process | glycolytic process | |
Biological Process | intracellular glucose homeostasis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHexokinase-2
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionQ2KNB9
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000247565 | 1-494 | Hexokinase-2 | |||
Sequence: MRKAAAAAVAAAAAVGVALLVRRQLREAKRWGRADAVLRELEERCAAPPGRLRQVADAMAVEMHAGLASEGGSKLKMIISYVDALPSGEEKGVFYALDLGGTNFRVLRVQLGGKEGRVIKQEHDEISIPPHLMTGGSNELFDFIASSLAKFVASEGEDFHLAEGRQRELGFTFSFPVKQTSIASGTLINWTKGFSIDETVGEDVVTELTKALERQGLDMKVTALINDTIGTLAGGRYDDNDVIAAVILGTGTNAAYVERANAIPKWHDLLPKSGDMVINMEWGNFRSSHLPLTEFDQALDAESLNPGEQVYEKLISGMYLGEIVRRVLLKMAEEASLFGDEVPPKLKIPFIIRTPYMSMMHCDRSPDLRTVGAKLKDILGVQNTSLKTRRLVVDVCDIVAKRAAHLAAAGIHGILKKLGRDVPNTDKQRTVIAVDGGLYEHYTIFAECVESTLRDMLGEDVSSTIVIKLAKDGSGIGAALLAAAHSQYREAEEL |
Proteomic databases
Expression
Tissue specificity
Expressed in roots, leaves, flowers, immature seeds, endosperm and seed coat.
Induction
By glucose or fructose treatment in leaves.
Developmental stage
Expressed during flower development until 15 days after flowering.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 32-483 | Hexokinase | ||||
Sequence: GRADAVLRELEERCAAPPGRLRQVADAMAVEMHAGLASEGGSKLKMIISYVDALPSGEEKGVFYALDLGGTNFRVLRVQLGGKEGRVIKQEHDEISIPPHLMTGGSNELFDFIASSLAKFVASEGEDFHLAEGRQRELGFTFSFPVKQTSIASGTLINWTKGFSIDETVGEDVVTELTKALERQGLDMKVTALINDTIGTLAGGRYDDNDVIAAVILGTGTNAAYVERANAIPKWHDLLPKSGDMVINMEWGNFRSSHLPLTEFDQALDAESLNPGEQVYEKLISGMYLGEIVRRVLLKMAEEASLFGDEVPPKLKIPFIIRTPYMSMMHCDRSPDLRTVGAKLKDILGVQNTSLKTRRLVVDVCDIVAKRAAHLAAAGIHGILKKLGRDVPNTDKQRTVIAVDGGLYEHYTIFAECVESTLRDMLGEDVSSTIVIKLAKDGSGIGAALLAA | ||||||
Region | 87-225 | Hexokinase small subdomain | ||||
Sequence: SGEEKGVFYALDLGGTNFRVLRVQLGGKEGRVIKQEHDEISIPPHLMTGGSNELFDFIASSLAKFVASEGEDFHLAEGRQRELGFTFSFPVKQTSIASGTLINWTKGFSIDETVGEDVVTELTKALERQGLDMKVTALI | ||||||
Region | 226-472 | Hexokinase large subdomain | ||||
Sequence: NDTIGTLAGGRYDDNDVIAAVILGTGTNAAYVERANAIPKWHDLLPKSGDMVINMEWGNFRSSHLPLTEFDQALDAESLNPGEQVYEKLISGMYLGEIVRRVLLKMAEEASLFGDEVPPKLKIPFIIRTPYMSMMHCDRSPDLRTVGAKLKDILGVQNTSLKTRRLVVDVCDIVAKRAAHLAAAGIHGILKKLGRDVPNTDKQRTVIAVDGGLYEHYTIFAECVESTLRDMLGEDVSSTIVIKLAKD |
Sequence similarities
Belongs to the hexokinase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length494
- Mass (Da)53,626
- Last updated2006-03-07 v1
- ChecksumCBD4744352238E0D
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q0DGH2 | Q0DGH2_ORYSJ | Os05g0532600 | 135 |
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ116384 EMBL· GenBank· DDBJ | AAZ93619.1 EMBL· GenBank· DDBJ | mRNA | ||
AY884166 EMBL· GenBank· DDBJ | AAX68419.1 EMBL· GenBank· DDBJ | mRNA | ||
AC121365 EMBL· GenBank· DDBJ | AAT47078.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AP014961 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |