Q2KJJ8 · PLD3_BOVIN
- Protein5'-3' exonuclease PLD3
- GenePLD3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids490 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA) (By similarity).
Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor in collaboration with PLD4 (By similarity).
May be important in myotube formation. Plays a role in lysosomal homeostasis. Involved in the regulation of endosomal protein sorting (By similarity).
Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor in collaboration with PLD4 (By similarity).
May be important in myotube formation. Plays a role in lysosomal homeostasis. Involved in the regulation of endosomal protein sorting (By similarity).
Catalytic activity
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 201 | |||||
Sequence: H | ||||||
Active site | 203 | |||||
Sequence: K | ||||||
Active site | 208 | |||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | early endosome membrane | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | Golgi membrane | |
Cellular Component | late endosome membrane | |
Cellular Component | lysosomal lumen | |
Cellular Component | lysosomal membrane | |
Molecular Function | single-stranded DNA 5'-3' DNA exonuclease activity | |
Biological Process | immune system process | |
Biological Process | inflammatory response | |
Biological Process | myotube differentiation | |
Biological Process | regulation of cytokine production involved in inflammatory response |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5'-3' exonuclease PLD3
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionQ2KJJ8
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass type II membrane protein
Early endosome membrane ; Single-pass type II membrane protein
Late endosome membrane ; Single-pass type II membrane protein
Golgi apparatus membrane ; Single-pass type II membrane protein
Endosome membrane ; Single-pass type II membrane protein
Note: Localizes to ER-associated vesicles in differentiating myotubes. The soluble form in lysosome arises by proteolytic processing of the membrane-bound form.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-38 | Cytoplasmic | ||||
Sequence: MKPKLMYQELKVPAEEPASELPMNEIEAWKAAEKKARW | ||||||
Transmembrane | 39-59 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: VLLVLILAVVGFGALMTQLFL | ||||||
Topological domain | 60-490 | Lumenal | ||||
Sequence: WEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGLDFPNASTSNPSTSQAWLGLLAGAHSSLDIASFYWTLTNNDTHTQEASAQQGEEVLRQLQTLAPRGVKVRIAVSKPNGPQPQADLQALLQSGAQVRMVDMQKLTHGVLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLARDLTKIFEAYWFLGQAGSSIPSTWPRPYDTRYNQETPMEICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDNARSFIYIAVMNYLPIMEFSHPRRFWPAIDDGLRRAAYERGVKVRLLISCWGHSDPSMRAFLLSLAALRDNHTHSDIQVKLFVVPADDAQARIPYARVNHNKYMVTERATYIGTSNWSGSYFTETAGTSLLVTQNGRGGLRSQLEAVFLRDWDSPYSHDLDAAADSVGNACRLL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000280325 | 1-490 | 5'-3' exonuclease PLD3 | |||
Sequence: MKPKLMYQELKVPAEEPASELPMNEIEAWKAAEKKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGLDFPNASTSNPSTSQAWLGLLAGAHSSLDIASFYWTLTNNDTHTQEASAQQGEEVLRQLQTLAPRGVKVRIAVSKPNGPQPQADLQALLQSGAQVRMVDMQKLTHGVLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLARDLTKIFEAYWFLGQAGSSIPSTWPRPYDTRYNQETPMEICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDNARSFIYIAVMNYLPIMEFSHPRRFWPAIDDGLRRAAYERGVKVRLLISCWGHSDPSMRAFLLSLAALRDNHTHSDIQVKLFVVPADDAQARIPYARVNHNKYMVTERATYIGTSNWSGSYFTETAGTSLLVTQNGRGGLRSQLEAVFLRDWDSPYSHDLDAAADSVGNACRLL | ||||||
Glycosylation | 132 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Post-translational modification
N-glycosylated.
Proteolytically processed to a soluble form that is stable within endosomes and lysosomes. During transport through the secretory pathway becomes proteolysed by cysteine proteases, thereby releasing a stable soluble lysosomal lumenal polypeptide, whereas the transmembrane-bound fragment is rapidly degraded. Its transport route to lysosomes involves ubiquitination and the ESCRT complex.
Ubiquitinated. Ubiquitination mediates sorting into lysosomes.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 196-223 | PLD phosphodiesterase 1 | ||||
Sequence: THGVLHTKFWVVDQTHFYLGSANMDWRS | ||||||
Domain | 411-437 | PLD phosphodiesterase 2 | ||||
Sequence: YARVNHNKYMVTERATYIGTSNWSGSY |
Sequence similarities
Belongs to the phospholipase D family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length490
- Mass (Da)54,619
- Last updated2006-03-07 v1
- Checksum8D5F78A548AD3BE8
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC105309 EMBL· GenBank· DDBJ | AAI05310.1 EMBL· GenBank· DDBJ | mRNA |