Q2KJJ8 · PLD3_BOVIN

  • Protein
    5'-3' exonuclease PLD3
  • Gene
    PLD3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA) (By similarity).
Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor in collaboration with PLD4 (By similarity).
May be important in myotube formation. Plays a role in lysosomal homeostasis. Involved in the regulation of endosomal protein sorting (By similarity).

Caution

It was initially thought that PDL3 has phospholipase D activity due to its HKD motifs. The second HKD motif contains Glu instead of the canonical Asp. Its enzyme activity is therefore unsure. Catalytic phospholipase D activity is still controversial (By similarity).
Its closest homolog PLD4, exhibits no phospholipase activity (By similarity).

Catalytic activity

  • Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates.
    EC:3.1.16.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

149050100150200250300350400450
TypeIDPosition(s)Description
Active site201
Active site203
Active site208

GO annotations

AspectTerm
Cellular Componentearly endosome membrane
Cellular Componentendoplasmic reticulum membrane
Cellular ComponentGolgi membrane
Cellular Componentlate endosome membrane
Cellular Componentlysosomal lumen
Cellular Componentlysosomal membrane
Molecular Functionsingle-stranded DNA 5'-3' DNA exonuclease activity
Biological Processimmune system process
Biological Processinflammatory response
Biological Processmyotube differentiation
Biological Processregulation of cytokine production involved in inflammatory response

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    5'-3' exonuclease PLD3
  • EC number
  • Alternative names
    • Choline phosphatase 3
    • Phosphatidylcholine-hydrolyzing phospholipase D3
    • Phospholipase D3 (PLD 3)

Gene names

    • Name
      PLD3

Organism names

  • Taxonomic identifier
  • Strain
    • Crossbred X Angus
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos

Accessions

  • Primary accession
    Q2KJJ8

Proteomes

Organism-specific databases

Subcellular Location

Endoplasmic reticulum membrane
; Single-pass type II membrane protein
Lysosome lumen
Early endosome membrane
; Single-pass type II membrane protein
Late endosome membrane
; Single-pass type II membrane protein
Golgi apparatus membrane
; Single-pass type II membrane protein
Endosome membrane
; Single-pass type II membrane protein
Note: Localizes to ER-associated vesicles in differentiating myotubes. The soluble form in lysosome arises by proteolytic processing of the membrane-bound form.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-38Cytoplasmic
Transmembrane39-59Helical; Signal-anchor for type II membrane protein
Topological domain60-490Lumenal

Keywords

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00002803251-4905'-3' exonuclease PLD3
Glycosylation132N-linked (GlcNAc...) asparagine

Post-translational modification

N-glycosylated.
Proteolytically processed to a soluble form that is stable within endosomes and lysosomes. During transport through the secretory pathway becomes proteolysed by cysteine proteases, thereby releasing a stable soluble lysosomal lumenal polypeptide, whereas the transmembrane-bound fragment is rapidly degraded. Its transport route to lysosomes involves ubiquitination and the ESCRT complex.
Ubiquitinated. Ubiquitination mediates sorting into lysosomes.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Interacts with APP.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain196-223PLD phosphodiesterase 1
Domain411-437PLD phosphodiesterase 2

Sequence similarities

Belongs to the phospholipase D family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    490
  • Mass (Da)
    54,619
  • Last updated
    2006-03-07 v1
  • Checksum
    8D5F78A548AD3BE8
MKPKLMYQELKVPAEEPASELPMNEIEAWKAAEKKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGLDFPNASTSNPSTSQAWLGLLAGAHSSLDIASFYWTLTNNDTHTQEASAQQGEEVLRQLQTLAPRGVKVRIAVSKPNGPQPQADLQALLQSGAQVRMVDMQKLTHGVLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLARDLTKIFEAYWFLGQAGSSIPSTWPRPYDTRYNQETPMEICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDNARSFIYIAVMNYLPIMEFSHPRRFWPAIDDGLRRAAYERGVKVRLLISCWGHSDPSMRAFLLSLAALRDNHTHSDIQVKLFVVPADDAQARIPYARVNHNKYMVTERATYIGTSNWSGSYFTETAGTSLLVTQNGRGGLRSQLEAVFLRDWDSPYSHDLDAAADSVGNACRLL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BC105309
EMBL· GenBank· DDBJ
AAI05310.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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