Q2HR71 · VIRF2_HHV8P
- ProteinViral IRF2-like protein
- GenevIRF-2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids680 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
DNA-binding transcription factor that plays a role in the modulation of host immune response (PubMed:26537687, PubMed:31059555).
Acts by interacting with host EIF2AK2/PKR and inhibiting its activation. In turn, EIF2AK2/PKR substrates including EIF2S1 or histone H2A are not phosphorylated (PubMed:11160738).
Inhibits type I interferon signaling by targeting host IRF3 during viral reactivation from latency (PubMed:11160738, PubMed:21697347).
Attenuates the transcriptional activity of host FOXO3 via activation of the AKT1 signaling pathway, inhibiting FOXO3-mediated apoptosis (PubMed:26797279).
Suppresses also the expression of viral early lytic genes in both newly infected and reactivated infected host cells allowing regulation of viral life cycle by harnessing the interferon pathway. Mechanistically, promotes host PML bodies formation as well as host antiviral restriction factors IFIT1-3 expression leading to inhibition of viral early lytic proteins (PubMed:31059555).
Regulates also host TRAF3 and TRAF6 ubiquitination by interacting with USP7 deubiquitinase thereby influencing TRAF3/6-mediated signal transduction (PubMed:31666375).
Acts by interacting with host EIF2AK2/PKR and inhibiting its activation. In turn, EIF2AK2/PKR substrates including EIF2S1 or histone H2A are not phosphorylated (PubMed:11160738).
Inhibits type I interferon signaling by targeting host IRF3 during viral reactivation from latency (PubMed:11160738, PubMed:21697347).
Attenuates the transcriptional activity of host FOXO3 via activation of the AKT1 signaling pathway, inhibiting FOXO3-mediated apoptosis (PubMed:26797279).
Suppresses also the expression of viral early lytic genes in both newly infected and reactivated infected host cells allowing regulation of viral life cycle by harnessing the interferon pathway. Mechanistically, promotes host PML bodies formation as well as host antiviral restriction factors IFIT1-3 expression leading to inhibition of viral early lytic proteins (PubMed:31059555).
Regulates also host TRAF3 and TRAF6 ubiquitination by interacting with USP7 deubiquitinase thereby influencing TRAF3/6-mediated signal transduction (PubMed:31666375).
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 7-103 | IRF tryptophan pentad repeat | ||||
Sequence: SEWLTDFIIDALDSGRFWGVGWLDEQKRIFTVPGRNRRERMPEGFDDFYEAFLEERRRHGLPEIPETETGLGCFGRLLRTANRARQERPFTIYKGKM |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell cytoplasm | |
Cellular Component | host cell nucleus | |
Molecular Function | protein serine/threonine kinase inhibitor activity | |
Molecular Function | transcription cis-regulatory region binding | |
Biological Process | symbiont-mediated perturbation of host apoptosis | |
Biological Process | symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity | |
Biological Process | symbiont-mediated suppression of host PKR/eIFalpha signaling | |
Biological Process | symbiont-mediated suppression of host type I interferon-mediated signaling pathway | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameViral IRF2-like protein
- Short namesvIRF-2
Gene names
Organism names
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Peploviricota > Herviviricetes > Herpesvirales > Orthoherpesviridae > Gammaherpesvirinae > Rhadinovirus > Rhadinovirus humangamma8 > Human herpesvirus 8
- Virus hosts
Accessions
- Primary accessionQ2HR71
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 82 | About 50% loss of transcriptional regulation and DNA-binding. | ||||
Sequence: R → A | ||||||
Mutagenesis | 85 | About 50% loss of transcriptional regulation and DNA-binding. | ||||
Sequence: R → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000423774 | 1-680 | Viral IRF2-like protein | |||
Sequence: MPRYTESEWLTDFIIDALDSGRFWGVGWLDEQKRIFTVPGRNRRERMPEGFDDFYEAFLEERRRHGLPEIPETETGLGCFGRLLRTANRARQERPFTIYKGKMKLNRWIMTPRPYKGCEGCLVYLTQEPAMKNMLKALFGIYPHDDKHREKALRRSLRKKAQREAARKQAAAVATPTTSSAAEVSSRSQSEDTESSDSENELWVGAQGFVGRDMHSLFFEEPEPSGFGSSGQSSSLLAPDSPRPSTSQVQGPLHVHTPTDLCLPTGGLPSPVIFPHETQGLLAPPAGQSQTPFSPEGPVPSHVSGLDDCLPMVDHIEGCLLDLLSDVGQELPDLGDLGELLCETASPQGPMQSEGGEEGSTESVSVLPATHPLESSAPGASVMGSGQELPDLGDLSELLCETASPQGPMQSEGGEEGSTESVSVLPATHPLESSAPGASVMGSSFQASDNVDDFIDCIPPLCRDDRDVEDQEKADQTFYWYGSDMRPKVLTATQSVAAYLSKKQAIYKVGDKLVPLVVEVYYFGEKVKTHFDLTGGIVICSQVPEASPEHICQTVPPYKCLLPRTAHCSVDANRTLEQTLDRFSMGVVAIGTNMGIFLKGLLEYPAYFVGNASRRRIGKCRPLSHRHEIQQAFDVERHNREPEGSRYASLFLGRRPSPEYDWDHYPVILHIYLAPFYHRD |
Interaction
Subunit
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | Q2HR71 | EIF2AK2 P19525 | 2 | EBI-8876177, EBI-640775 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 156-201 | Disordered | ||||
Sequence: SLRKKAQREAARKQAAAVATPTTSSAAEVSSRSQSEDTESSDSENE | ||||||
Compositional bias | 172-191 | Polar residues | ||||
Sequence: AVATPTTSSAAEVSSRSQSE | ||||||
Region | 220-257 | Disordered | ||||
Sequence: EEPEPSGFGSSGQSSSLLAPDSPRPSTSQVQGPLHVHT | ||||||
Compositional bias | 226-254 | Polar residues | ||||
Sequence: GFGSSGQSSSLLAPDSPRPSTSQVQGPLH | ||||||
Region | 343-365 | Disordered | ||||
Sequence: ETASPQGPMQSEGGEEGSTESVS | ||||||
Compositional bias | 346-365 | Polar residues | ||||
Sequence: SPQGPMQSEGGEEGSTESVS | ||||||
Region | 403-423 | Disordered | ||||
Sequence: ASPQGPMQSEGGEEGSTESVS |
Sequence similarities
Belongs to the IRF family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length680
- Mass (Da)75,090
- Last updated2006-03-21 v1
- Checksum0FBC22C8F51F3084
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 172-191 | Polar residues | ||||
Sequence: AVATPTTSSAAEVSSRSQSE | ||||||
Compositional bias | 226-254 | Polar residues | ||||
Sequence: GFGSSGQSSSLLAPDSPRPSTSQVQGPLH | ||||||
Compositional bias | 346-365 | Polar residues | ||||
Sequence: SPQGPMQSEGGEEGSTESVS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF148805 EMBL· GenBank· DDBJ | ABD28912.1 EMBL· GenBank· DDBJ | Genomic DNA |