Q2GG85 · Q2GG85_EHRCR

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

Type
IDPosition(s)Description
Site31Interaction with DNA
Site143Interaction with DNA
Site144Interaction with DNA
Site147Interaction with DNA
Site159Interaction with DNA
Active site305O-(5'-phospho-DNA)-tyrosine intermediate
Site307Interaction with DNA
Site498Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • Ordered locus names
      ECH_0750

Organism names

Accessions

  • Primary accession
    Q2GG85

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain1-117Toprim
Region167-172Interaction with DNA

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    820
  • Mass (Da)
    92,567
  • Last updated
    2006-03-21 v1
  • Checksum
    19C92960C9E38A4E
MSLIIVESPSKAKTISKYLGSKYKVVASFGHIRDFPAKSGSVDPDKDFAMTYEIISKSEKYVNKLIQVANKETQGIYLATDPDREGEAIAWHIIEVLKENNAIDDNVVINRMVFNEVTKSAILESIKRPRSINMDLVYAQQARRALDYLVGFTLSPLLWRKLPGSKSAGRVQSVALRLICDRESDIEKFITQEYWDIEAKCCNTEGKQFSAFLSCYAGKKLEKFDIKNEEDAQKLSEEVRSRSYSVINVEKKHTKRNPYPPFITSSLQQEASTKLGFTAKNTMLIAQKLYEGIDVGGEIVGLITYMRTDGFYIADEALEHIRSIIVSMFGKEYLPESARKYVKKVKNAQEAHEAIRPTNITITPGSLSNYLTPEQLKLYDLIWKRTVASQMNSAVIDQVIVELGSLDKVVTLRATGSSLFFDGFYKVYGHGEDDDSKNNMLPLLQKNDKCPLDEVVPHQHFTNPPARYNEASLVKKMEEIGIGRPSTYASIISVLQDRQYVILNQKRFFPTERGRIVNVFLVNFFSRYVEYDFTADLEEKLDLISNGKINWKEVLHQFWFLFIDNVNSVKKLEFTEVLKVISCEMENYIFSSDKSESSRVCPVCNDGTLELNISKNGIFLGCSKYPECKYTKNVGSGIVDDTDNTQYPKILGIDAATGQEIYLKKGPYGLYLQIGNDKSGKRAAIPKNIDANLLDLQMAEKILSLPISLGTHPDTGQEIKLGLGKFGAYILYGGKYFSVKNEHNFLDISIENAINIIANNSTSGVIKSLGITEDNKEVFLCKGRYGFYLKYDKLNVALKKDTDIEQISLSDAIKLISEKL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000236
EMBL· GenBank· DDBJ
ABD44526.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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