Q2G2U9 · SARA_STAA8

Function

function

Global regulator with both positive and negative effects that controls expression of several virulence factors and biofilm formation process in a cell density-dependent manner. In a strain-dependent manner plays a role in multidrug resistance mechanism. Is required for transcription of primary transcripts RNAII and RNAIII generated by agr (virulence accessory gene regulator) locus. Acts as a transcriptional activator of the genes encoding, among others, for fibronectin binding proteins (fnbA and fnbB), hemolysins (hla, hld, hlgB and hlgC), serine proteases (splA, splB, splD and splF) and of the bap gene, which is essential for biofilm development in some strains. Negatively regulates the expression of the genes for protein A (spa), lipase (lip), thermonuclease (nuc), immunodominant staphylococcal antigen B (isaB), staphylococcal serine and cysteine proteases (sspA and sspB), staphostatin B (sspC), metalloprotease aureolysin (aur) and collagen adhesin (cna). Probably activates the development of biofilm by both enhancing the ica operon transcription and suppressing the transcription of either a protein involved in the turnover of PIA/PNAG or a repressor of its synthesis, whose expression would be sigma-B-dependent.

Miscellaneous

Can regulate transcription of some virulence factors (hemolysins, staphylococcal serine and cysteine proteases, lipase, protein A and metalloprotease aureolysin) via either agr-dependent and/or agr-independent pathway. Agr and SarA have opposing effects on the expression of sspA, sspB, sspC, lip and aur.
The 11 C-terminal amino acids are absent in substrain NCTC 8325 / RN6390 (AAB05396) due to a nonsense mutation (UGA). However, this truncated SarA protein is functional.
The regulatory events observed in strain NCTC 8325 / RN6390 are not representative of the events that occur in clinical isolates of S.aureus.
Strains NCTC 8325 and NCTC 8325 / RN6390 are able to infect and kill Arabidopsis thaliana. This effect is attenuated in sarA and agr mutant backgrounds.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site7a divalent metal cation (UniProtKB | ChEBI)
Binding site8a divalent metal cation (UniProtKB | ChEBI)
Binding site11a divalent metal cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionDNA binding
Molecular Functionmetal ion binding
Biological Processregulation of DNA-templated transcription
Biological Processresponse to stress

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Transcriptional regulator SarA
  • Alternative names
    • Staphylococcal accessory regulator A

Gene names

    • Name
      sarA
    • Ordered locus names
      SAOUHSC_00620

Organism names

Accessions

  • Primary accession
    Q2G2U9
  • Secondary accessions
    • Q53600
    • Q53777

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00002493232-124Transcriptional regulator SarA

Proteomic databases

Expression

Induction

Expressed at the exponential growth phase and maximally induced during the transition from late exponential phase to stationary phase. Repressed by SarR and activated by two-component regulatory system ArlS/ArlR. Activated by SigB in strains harboring an intact sigB operon (rsbU, rsbV, rsbW, and sigB). Transcription is also attenuated by MsrR.

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the SarA family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    124
  • Mass (Da)
    14,718
  • Last updated
    2007-01-23 v3
  • Checksum
    DB9A16E806C10661
MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYISENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRKKIESLLSRVNKRITEANNEIEL

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict53in Ref. 2; AAB05396
Sequence conflict114-124in Ref. 2; AAB05396

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U20782
EMBL· GenBank· DDBJ
AAA62477.1
EMBL· GenBank· DDBJ
Genomic DNA
U46541
EMBL· GenBank· DDBJ
AAB05396.1
EMBL· GenBank· DDBJ
Genomic DNA
CP000253
EMBL· GenBank· DDBJ
ABD29758.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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