Q2G297 · AP4AH_STAA8

Function

function

Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) to yield ADP (PubMed:32152217).
Can also hydrolyze Ap3A, Ap5A, Ap4G, Ap4U and Gp4G, always releasing ADP or GDP as one of the products, but it exhibits a marked preference for Ap4A, which is mainly exerted at the substrate affinity level (PubMed:32152217).

Catalytic activity

Activity regulation

Inhibited by EDTA.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
3.2 μMAp4A
21.5 μMAp5A
19.7 μMAp4G
17.6 μMAp4U
68 μMGp4G
kcat is 107 sec-1 with Ap4A as substrate. kcat is 32 sec-1 with Ap5A as substrate. kcat is 210 sec-1 with Ap4G as substrate. kcat is 324 sec-1 with Ap4U as substrate. kcat is 1002 sec-1 with Gp4G as substrate.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site21ADP (UniProtKB | ChEBI)
Binding site21Fe cation (UniProtKB | ChEBI)
Binding site50Fe cation (UniProtKB | ChEBI)
Binding site51Fe cation (UniProtKB | ChEBI)
Binding site51-54ADP (UniProtKB | ChEBI)
Binding site83ADP (UniProtKB | ChEBI)
Binding site109-110ADP (UniProtKB | ChEBI)
Binding site127ADP (UniProtKB | ChEBI)
Binding site127Fe cation (UniProtKB | ChEBI)
Binding site133ADP (UniProtKB | ChEBI)
Binding site172-177ADP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionhydrolase activity
Molecular Functionmetal ion binding
Molecular Functionnucleotide binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bis(5'-nucleosyl)-tetraphosphatase, symmetrical
  • EC number
  • Alternative names
    • Ap4A hydrolase
    • Symmetrically cleaving Ap4A hydrolase

Gene names

    • Name
      yqeK
    • Ordered locus names
      SAOUHSC_01696

Organism names

Accessions

  • Primary accession
    Q2G297

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004547781-194Bis(5'-nucleosyl)-tetraphosphatase, symmetrical

Proteomic databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain18-132HD

Sequence similarities

Belongs to the Ap4A hydrolase YqeK family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    194
  • Mass (Da)
    22,464
  • Last updated
    2006-03-21 v1
  • Checksum
    15F2A27F5B5D55FF
MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVELAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEVLMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISKRTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000253
EMBL· GenBank· DDBJ
ABD30770.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp