Q2FYQ8 · NIKE_STAA8
- ProteinNickel import system ATP-binding protein NikE
- GenenikE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids233 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Part of the ABC transporter complex NikABCDE (Opp2) involved in nickel import. Probably responsible for energy coupling to the transport system. Required for full urease activity and plays a significant role in the virulence of S.aureus during urinary tract infection (UTI).
Catalytic activity
- ATP + H2O + Ni2+(out) = ADP + H+ + Ni2+(in) + phosphateThis reaction proceeds in the forward direction.
CHEBI:30616 + CHEBI:15377 + Ni2+ (out)CHEBI:49786= CHEBI:456216 + CHEBI:15378 + Ni2+ (in)CHEBI:49786+ CHEBI:43474
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | ABC-type nickel transporter activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | transmembrane transporter activity | |
Biological Process | transmembrane transport |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNickel import system ATP-binding protein NikE
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionQ2FYQ8
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000272188 | 1-233 | Nickel import system ATP-binding protein NikE | |||
Sequence: MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTLASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTAQDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDVIAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNVDRHPYTKELVQAFSY |
Proteomic databases
Expression
Induction
Transcription is stable during the exponential phase and increases only in standard conditions in late exponential phase.
Interaction
Subunit
The complex is composed of two ATP-binding proteins (NikD and NikE), two transmembrane proteins (NikB and NikC) and a solute-binding protein (NikA).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 2-228 | ABC transporter | ||||
Sequence: IELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTLASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTAQDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDVIAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNVDRHPYTKELV |
Sequence similarities
Belongs to the ABC transporter superfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length233
- Mass (Da)26,257
- Last updated2006-03-21 v1
- ChecksumC3B38342B3DE9673
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 203 | in Ref. 2; AAO47758 | ||||
Sequence: D → N |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF076684 EMBL· GenBank· DDBJ | AAC69846.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY205146 EMBL· GenBank· DDBJ | AAO47758.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP000253 EMBL· GenBank· DDBJ | ABD30472.1 EMBL· GenBank· DDBJ | Genomic DNA |