Q2FIF3 · NMO_STAA3
- ProteinProbable nitronate monooxygenase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids355 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Nitronate monooxygenase that uses molecular oxygen to catalyze the oxidative denitrification of alkyl nitronates. Acts on propionate 3-nitronate (P3N), the presumed physiological substrate. Probably functions in the detoxification of P3N, a metabolic poison produced by plants and fungi as a defense mechanism.
Miscellaneous
P3N is a potent irreversible inhibitor of the key enzyme succinate dehydrogenase in the Krebs cycle and electron transport chain. P3N has been shown to be a toxic metabolite to bacteria, plants, fungi, mammals or any organism that uses succinate dehydrogenase.
Catalytic activity
- 3 propionate 3-nitronate + 3 O2 + H2O = 3 3-oxopropanoate + 2 nitrate + nitrite + H2O2 + 3 H+
Cofactor
Note: Binds 1 FMN per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 71 | FMN (UniProtKB | ChEBI) | |||
Binding site | 175 | FMN (UniProtKB | ChEBI) | |||
Binding site | 180 | FMN (UniProtKB | ChEBI) | |||
Binding site | 218 | FMN (UniProtKB | ChEBI) | |||
Binding site | 237-240 | FMN (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | nitronate monooxygenase activity | |
Molecular Function | nucleotide binding | |
Biological Process | response to toxic substance |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable nitronate monooxygenase
- EC number
- Short namesNMO
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionQ2FIF3
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000360901 | 1-355 | Probable nitronate monooxygenase | ||
Structure
Sequence
- Sequence statusComplete
- Length355
- Mass (Da)38,548
- Last updated2006-03-21 v1
- Checksum2ABD713D5FDA7DB6
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000255 EMBL· GenBank· DDBJ | ABD22280.1 EMBL· GenBank· DDBJ | Genomic DNA |