Q29558 · MAOX_PIG
- ProteinNADP-dependent malic enzyme
- GeneME1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids557 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP+ and divalent metal ions, and decarboxylation of oxaloacetate.
Catalytic activity
- (S)-malate + NADP+ = CO2 + NADPH + pyruvateThis reaction proceeds in the forward and the backward directions.
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Divalent metal cations. Prefers magnesium or manganese.
Features
Showing features for active site, binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 88 | Proton donor | ||||
Sequence: Y | ||||||
Binding site | 141 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Active site | 159 | Proton acceptor | ||||
Sequence: K | ||||||
Binding site | 231 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 232 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 255 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 255 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Site | 255 | Important for activity | ||||
Sequence: D | ||||||
Binding site | 394 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Molecular Function | magnesium ion binding | |
Molecular Function | malate dehydrogenase (decarboxylating) (NADP+) activity | |
Molecular Function | malic enzyme activity | |
Molecular Function | manganese ion binding | |
Molecular Function | NAD binding | |
Molecular Function | oxaloacetate decarboxylase activity | |
Biological Process | malate metabolic process | |
Biological Process | protein homotetramerization | |
Biological Process | pyruvate metabolic process | |
Biological Process | response to hormone |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADP-dependent malic enzyme
- EC number
- Short namesNADP-ME
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Suina > Suidae > Sus
Accessions
- Primary accessionQ29558
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000160194 | 1-557 | NADP-dependent malic enzyme | |||
Sequence: GYGLTRIPHLNKDLAFTLEERQQLNIHGLLPPCFISQDIQVLRVIKNFERLNSDFDRYLLLMDLQDRNEKLFYKVLMSDIEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDRGHVASVLNAWPEDVIKAVVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGVNPQECLPVILDVGTENEELLKDPLYIGLRQRRVRGPEYDDFLDEFMEAVSSKYGMNCLIQFEDFANINAFRLLKKYQNQYCTFNDDIQGTASVAVAGILAALRITKNKLSDQTILFQGAGEAALGIAHLIVMAMEKEGVPKEKAIKKIWLVDSKGLIVKGRAALTNEKEEFAHEHEEMKNLEAIVQDIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECTAERGYTLTQGRAIFASGSPFDPVTLPSGQTLYPGQGNNSYVFPGVALAVVACGLRHITDKIFLTTAEVIAQQVSDKHLEEGRLYPPLNTIRDVSLKIAEKIVRDAYQEKTATIYPEPSNKEAFVRSQMYSTDYDQILPDGYSWPEEAQKIQTKLD | ||||||
Modified residue | 322 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
Structure
Sequence
- Sequence statusFragment
- Length557
- Mass (Da)62,006
- Last updated1996-11-01 v1
- Checksum40692545F044BA99
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: G |
Keywords
- Technical term