Q29552 · C11B1_PIG
- ProteinCytochrome P450 11B1, mitochondrial
- GeneCYP11B1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids503 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
A cytochrome P450 monooxygenase that catalyzes the biosynthesis of aldosterone and other adrenal corticoids. Differing from other species (such as human, rat and mice), it is able to catalyze three sequential oxidative reactions of 11-deoxycorticosterone (21-hydroxyprogesterone), namely 11-beta hydroxylation, followed by two successive oxidations at C18 yielding 18-hydroxy and then 18-oxo intermediates, and ending with the formation of aldosterone. Steroid 11beta, 18- and 19-hydroxylase. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
Catalytic activity
- a steroid + 2 H+ + O2 + 2 reduced [adrenodoxin] = an 11beta-hydroxysteroid + H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
- 11-deoxycortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = cortisol + H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
- 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = corticosterone + H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
- 18-hydroxycorticosterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = aldosterone + 2 H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
- 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = 19-hydroxy-11-deoxycorticosterone + H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
- 19-hydroxy-11-deoxycorticosterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = 19-oxo-11-deoxycorticosterone + 2 H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
Cofactor
Pathway
Steroid biosynthesis; glucocorticoid biosynthesis.
Steroid hormone biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial inner membrane | |
Molecular Function | corticosterone 18-monooxygenase activity | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | steroid 11-beta-monooxygenase activity | |
Biological Process | aldosterone biosynthetic process | |
Biological Process | cellular response to peptide hormone stimulus | |
Biological Process | cholesterol metabolic process | |
Biological Process | cortisol metabolic process | |
Biological Process | glucocorticoid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome P450 11B1, mitochondrial
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Suina > Suidae > Sus
Accessions
- Primary accessionQ29552
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion inner membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-24 | Mitochondrion | ||||
Sequence: MAIWAKAEAWLAGPWLALNRARTL | ||||||
Chain | PRO_0000003602 | 25-503 | Cytochrome P450 11B1, mitochondrial | |||
Sequence: GTRAVLAPKGVLPFEAIPQFPGKKWMRVLQLWREQGFENNHLEMHQTFQELGPIFRFDVGGRNMVLVMLPEDVERCQKVEGLHPQRDVPGPWLAYRHLRGHKCGVFLLNGPTWRLDRLQLNPGVLSLQAMQKFTPLVDGVARDFSQALRARVMQNARGSLTLDIKPSIFRYTIEASNLVLFGERLGLLAHQPNPESLDFIHALEVMFKSTVQLMFMPRSLSRWTSTGTWKEHFEAWDCIFQYANKAIQRLYQELTLGHPWHYSGVVAELLTHANMTVDAIKANSIDLTAGSVDTTAYPLLMTLFELARNPEVQQALRQESLAAAARISENPQKAITELPLLRAALKETLRLYPVGIFLDRCVTSDLVLQNYHIPAGTLVKVLLYSLGRNPAVFARPERYHPQRWLDNQGSGTRFPHLAFGFGMRQCLGRRLAQVEMLLLLHHVLKNFLVETLVQEDIKMIYRFIMTPSTLPLLTFRAIS |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the cytochrome P450 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length503
- Mass (Da)57,340
- Last updated1997-11-01 v1
- Checksum4C40B1EEAAC49B05
Keywords
- Technical term