Q28036 · SL9A1_BOVIN
- ProteinSodium/hydrogen exchanger 1
- GeneSLC9A1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids818 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Electroneutral Na+ /H+ antiporter that extrudes Na+ in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism (By similarity).
Exchanges intracellular H+ ions for extracellular Na+ in 1:1 stoichiometry (By similarity).
Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival. In addition, can transport lithium Li+ and functions also as a Na+/Li+ antiporter. SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs (By similarity).
Exchanges intracellular H+ ions for extracellular Na+ in 1:1 stoichiometry (By similarity).
Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival. In addition, can transport lithium Li+ and functions also as a Na+/Li+ antiporter. SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs (By similarity).
Miscellaneous
Predicted models used for more than 20 years predicted 10-12 transmembrane segments. More recently, the structure of SLC9A1 has been solved and reveals that SLC9A1 possesses 13 transmembrane regions.
Catalytic activity
- Na+(in) + H+(out) = Na+(out) + H+(in)
- Li+(out) + H+(in) = Li+(in) + H+(out)
- Li+(in) + Na+(out) = Li+(out) + Na+(in)
Activity regulation
Activated at acidic pHs. Inhibited by amiloride and 5-amino-substituted derivatives. Inhibited by cariporide and eniporide. Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3) bind and differentially regulate SLC9A1 activity.
Features
Showing features for site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Site | 161 | Channel pore-lining | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | basolateral plasma membrane | |
Cellular Component | cytoplasm | |
Cellular Component | plasma membrane | |
Molecular Function | calcium-dependent protein binding | |
Molecular Function | calmodulin binding | |
Molecular Function | potassium:proton antiporter activity | |
Molecular Function | sodium:proton antiporter activity | |
Biological Process | cellular response to acidic pH | |
Biological Process | potassium ion transmembrane transport | |
Biological Process | protein complex oligomerization | |
Biological Process | regulation of intracellular pH | |
Biological Process | regulation of pH | |
Biological Process | response to acidic pH | |
Biological Process | sodium ion export across plasma membrane | |
Biological Process | sodium ion import across plasma membrane |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSodium/hydrogen exchanger 1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionQ28036
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Basolateral cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-98 | Extracellular | |||
Transmembrane | 99-121 | Helical; Name=1 | |||
Topological domain | 122-130 | Cytoplasmic | |||
Transmembrane | 131-148 | Helical; Name=2 | |||
Topological domain | 149-158 | Extracellular | |||
Transmembrane | 159-176 | Helical; Name=3 | |||
Topological domain | 177-186 | Cytoplasmic | |||
Transmembrane | 187-215 | Helical; Name=4 | |||
Topological domain | 216-222 | Extracellular | |||
Transmembrane | 223-249 | Helical; Name=5 | |||
Topological domain | 250-252 | Cytoplasmic | |||
Transmembrane | 253-283 | Helical; Name=6 | |||
Topological domain | 284-287 | Extracellular | |||
Transmembrane | 288-322 | Helical; Name=7 | |||
Topological domain | 323-328 | Cytoplasmic | |||
Transmembrane | 329-341 | Helical; Name=8 | |||
Topological domain | 342-350 | Extracellular | |||
Transmembrane | 351-371 | Helical; Name=9 | |||
Topological domain | 372-373 | Cytoplasmic | |||
Transmembrane | 374-404 | Helical; Name=10 | |||
Topological domain | 405-410 | Extracellular | |||
Transmembrane | 411-438 | Helical; Name=11 | |||
Topological domain | 439-444 | Cytoplasmic | |||
Transmembrane | 445-469 | Helical; Name=12 | |||
Topological domain | 470-475 | Extracellular | |||
Transmembrane | 476-505 | Helical; Name=13 | |||
Topological domain | 506-818 | Cytoplasmic | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000052345 | 1-818 | Sodium/hydrogen exchanger 1 | ||
Glycosylation | 75 | N-linked (GlcNAc...) asparagine | |||
Modified residue | 599 | Phosphoserine | |||
Modified residue | 602 | Phosphoserine | |||
Modified residue | 603 | Phosphothreonine | |||
Modified residue | 605 | Phosphoserine | |||
Modified residue | 648 | Phosphoserine | |||
Modified residue | 693 | Phosphoserine | |||
Modified residue | 697 | Phosphoserine | |||
Modified residue | 703 | Phosphoserine | |||
Modified residue | 723 | Phosphoserine | |||
Modified residue | 726 | Phosphoserine | |||
Modified residue | 729 | Phosphoserine | |||
Modified residue | 782 | Phosphothreonine | |||
Modified residue | 788 | Phosphoserine | |||
Modified residue | 790 | Phosphoserine | |||
Modified residue | 799 | Phosphoserine | |||
Post-translational modification
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1.
O-glycosylated.
Palmitoylated; may play a major role in SLC9A1 regulation.
Phosphorylation at Thr-782 increases SLC9A1 activity. Specifically dephosphorylated at Thr-782 by PPP3CA that negatively regulates SLC9A1 activity. Phosphorylation at Ser-648 by AKT1 reduces SLC9A1 binding to CALM1.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Homodimer; dimerization is crucial for its function (By similarity).
Oligomer (By similarity).
Interacts with CALM in a calcium-dependent manner (By similarity).
Interacts with TESC (By similarity).
Interacts (via the juxtamembrane region of the cytoplasmic C-terminal domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner (By similarity).
Interacts with CHP2; the interaction occurs in a calcium-dependent manner (By similarity).
Interacts with EZR; regulates the cytoskeletal interactions of SLC9A1 and promotes stress fiber formation (By similarity).
Oligomer (By similarity).
Interacts with CALM in a calcium-dependent manner (By similarity).
Interacts with TESC (By similarity).
Interacts (via the juxtamembrane region of the cytoplasmic C-terminal domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner (By similarity).
Interacts with CHP2; the interaction occurs in a calcium-dependent manner (By similarity).
Interacts with EZR; regulates the cytoskeletal interactions of SLC9A1 and promotes stress fiber formation (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 44-76 | Disordered | |||
Region | 503-545 | Interaction with TESC | |||
Region | 509-516 | PI(4,5)P2-binding region | |||
Region | 515-545 | Interaction with CHP2 | |||
Region | 540-545 | Confers pH-dependent PI(4,5)P2 binding | |||
Region | 552-560 | PI(4,5)P2-binding region | |||
Region | 633-818 | Interaction with CALM1 | |||
Region | 633-818 | Interaction with TESC | |||
Region | 684-687 | Interaction with PPP3CA | |||
Region | 715-720 | Interaction with PPP3CA | |||
Region | 741-818 | Disordered | |||
Compositional bias | 770-797 | Polar residues | |||
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length818
- Mass (Da)90,976
- Last updated2023-09-13 v2
- Checksum63831EE3F0FF0262
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 759-764 | in Ref. 1; AAA91483 | |||
Compositional bias | 770-797 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
NKLS02000002 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
U49432 EMBL· GenBank· DDBJ | AAA91483.1 EMBL· GenBank· DDBJ | mRNA |