Q27SA8 · Q27SA8_PHRHE

Function

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

GO annotations

AspectTerm
Cellular ComponentDNA recombinase complex
Cellular Componentendodeoxyribonuclease complex
Cellular Componentnucleus
Molecular Functiondouble-stranded DNA endonuclease activity
Molecular Functionmetal ion binding
Molecular Functionprotein homodimerization activity
Molecular Functionsequence-specific DNA binding
Molecular Functionubiquitin protein ligase activity
Biological Processadaptive immune response
Biological Processchromatin organization
Biological Processpre-B cell allelic exclusion
Biological ProcessV(D)J recombination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    V(D)J recombination-activating protein 1
  • EC number

Gene names

    • Name
      RAG-1

Organism names

Accessions

  • Primary accession
    Q27SA8

Subcellular Location

Keywords

  • Cellular component

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain243-282RING-type
Domain304-333RAG1-type
Domain344-350NBD

Domain

The NBD (nonamer binding) DNA-binding domain mediates the specific binding to the nonamer RSS motif by forming a tightly interwoven homodimer that binds and synapses 2 nonamer elements, with each NBD making contact with both DNA molecules. Each RSS is composed of well-conserved heptamer (consensus 5'-CACAGTG-3') and nonamer (consensus 5'-ACAAAAACC-3') sequences separated by a spacer of either 12 bp or 23 bp.

Sequence similarities

Belongs to the RAG1 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    350
  • Mass (Da)
    39,997
  • Last updated
    2006-04-04 v1
  • Checksum
    22A67CC54E6BF194
KGKVAATLDKVIEKEIETVSLMSKTPFETDTELNKGMQTTDKSALYMSQREVETHQANLQHLCRFCGGSFKTDPYKRSHPVHGPVDDETQALLRKKEKKATSWPDLLAKVFKTDVKGDIDTIHPTKFCHQCWTVVQKKLNNFPCEMFFPKKSLVEWHPHSPSCDVCGASFHGVKRKRKVLNPQLNKKLRMVTRSARKKRQIRNPKQVNQKSLVKMIASCKKIHLTTKILAVDYPADFVKSISCQICEHILADPVETTCKHLFCRACILKCLKIMGSYCPACRYPCFPTDLVGPVKSFLTILNSLPVRCPVKDCQEEVCLGKYCHHLSRHKEVEDKEGYVYINKGGRPRQH

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1
Non-terminal residue350

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DQ385412
EMBL· GenBank· DDBJ
ABD49417.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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