Q27GK7 · TPR4_ARATH
- ProteinTopless-related protein 4
- GeneTPR4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1135 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription corepressor of Zinc finger transcription factors GAF1/IDD2 and ENY/IDD1 in regulation of gibberellin homeostasis and signaling.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | transcription corepressor activity | |
Biological Process | gibberellic acid homeostasis | |
Biological Process | negative regulation of gibberellic acid mediated signaling pathway | |
Biological Process | negative regulation of transcription by RNA polymerase II | |
Biological Process | primary shoot apical meristem specification |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameTopless-related protein 4
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ27GK7
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000394735 | 1-1135 | Topless-related protein 4 | |||
Sequence: MSSLSRELVFLILQFLDEEKFKDTVHRLEKESGFFFNMRYFEDSVTAGEWDDVEKYLSGFTKVDDNRYSMKIFFEIRKQKYLEALDKKDHAKAVDILVKELKVFSTFNEELFKEITMLLTLTNFRENEQLSKYGDTKSARGIMLGELKKLIEANPLFRDKLQFPSLKNSRLRTLINQSLNWQHQLCKNPRPNPDIKTLFVDHTCGHPNGAHTPSPTTNHLMGSVPKVGGFPPLGAHGPFQPTPAPLTTSLAGWMPNPSVQHPTVSAGPIGLGAPNSAVSMLKRERPRSPPTNSLSMDYQTADSESVLKRPRPFGISDGVNNLPVNVLPVTYPGQSHAHATYSTDDLPKNVSRILSQGSAIKSMDFHPVQQTMLLVGTNLGDIAIWEVGSREKLVSRSFKVWDLATCTVNLQASLASEYTAAVNRVVWSPDGGLLGVAYSKHIVHIYSYHGGEDLRNHLEIDAHAGNVNDLAFSQPNQQLCVVTCGEDKTIKVWDAVTGNKLHTFEGHEAPVYSVCPHQKENIQFIFSTAVDGKIKAWLYDNMGSRVDYDAPGRSCTSMAYCADGTRLFSCGTSKEGESFIVEWNESEGAVKRTYLGLGKRSVGVVQFDTMKNKFLVAGDEFQVKFWDMDSVDLLSSTAAEGGLPSSPCLRINKEGTLLAVSTTDNGIKILANAEGSRILHSMANRGLDSSRAPPGSVAKGPIVGTFGTPNSSTGMSLSMGERSGPVASVTGLNGDNRSLPDVKPRIADDAEKSKTWKLTEISERSQLRTLRLPDTLLPARVVKLIYTNSGGAILALAENAAHKLWKWQKSERNLLGKANSNVPPQLWQPSSGVLMTNDTREGNKEDVVPCFALSKNDSYVMSASGGKISLFNMMTFKTMTTFMAPPPAATSLAFHPQDNNIIAIGMDDSSIQIYNVRVDEVKSKLKGHQKRVTGLAFSNVLNVLVSSGADSQLCVWSMDGWEKQASKQIQIPSGHSPNPLAHTRVQFHQDQIHVLVVHASQLAIYEAPKLENMKQWIPKESSGSVTDAVYSCDSQSIYAAFDDGSVSILTATTLQLKCRIGPNSYLPSNPSSRVYPATVAAHPSEPNQFAVGLTDGGVHVIEPPGPEGKWGISAPPENGAGPSVSSAPGSDQQPR | ||||||
Modified residue | 214 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Tetramer (PubMed:26601214).
Interacts with WUS (via the C-terminal domain) (PubMed:16461579).
Interacts with SPL (via EAR motif) (PubMed:25378179, PubMed:25527103).
Interacts with SPEAR3/TIE1 (PubMed:23444332).
Binds to and corepresses GAF1/IDD2 at the promoter of GA20OX2 gene (PubMed:25035403).
Interacts with WUS (via the C-terminal domain) (PubMed:16461579).
Interacts with SPL (via EAR motif) (PubMed:25378179, PubMed:25527103).
Interacts with SPEAR3/TIE1 (PubMed:23444332).
Binds to and corepresses GAF1/IDD2 at the promoter of GA20OX2 gene (PubMed:25035403).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 4-36 | LisH | ||||
Sequence: LSRELVFLILQFLDEEKFKDTVHRLEKESGFFF | ||||||
Domain | 34-92 | CTLH | ||||
Sequence: FFFNMRYFEDSVTAGEWDDVEKYLSGFTKVDDNRYSMKIFFEIRKQKYLEALDKKDHAK | ||||||
Region | 281-303 | Disordered | ||||
Sequence: LKRERPRSPPTNSLSMDYQTADS | ||||||
Compositional bias | 286-302 | Polar residues | ||||
Sequence: PRSPPTNSLSMDYQTAD | ||||||
Repeat | 355-395 | WD 1 | ||||
Sequence: SQGSAIKSMDFHPVQQTMLLVGTNLGDIAIWEVGSREKLVS | ||||||
Repeat | 417-456 | WD 2 | ||||
Sequence: EYTAAVNRVVWSPDGGLLGVAYSKHIVHIYSYHGGEDLRN | ||||||
Repeat | 462-503 | WD 3 | ||||
Sequence: AHAGNVNDLAFSQPNQQLCVVTCGEDKTIKVWDAVTGNKLHT | ||||||
Repeat | 506-547 | WD 4 | ||||
Sequence: GHEAPVYSVCPHQKENIQFIFSTAVDGKIKAWLYDNMGSRVD | ||||||
Repeat | 550-593 | WD 5 | ||||
Sequence: APGRSCTSMAYCADGTRLFSCGTSKEGESFIVEWNESEGAVKRT | ||||||
Repeat | 597-636 | WD 6 | ||||
Sequence: LGKRSVGVVQFDTMKNKFLVAGDEFQVKFWDMDSVDLLSS | ||||||
Repeat | 638-680 | WD 7 | ||||
Sequence: AAEGGLPSSPCLRINKEGTLLAVSTTDNGIKILANAEGSRILH | ||||||
Repeat | 776-815 | WD 8 | ||||
Sequence: LLPARVVKLIYTNSGGAILALAENAAHKLWKWQKSERNLL | ||||||
Repeat | 843-881 | WD 9 | ||||
Sequence: NKEDVVPCFALSKNDSYVMSASGGKISLFNMMTFKTMTT | ||||||
Repeat | 884-924 | WD 10 | ||||
Sequence: APPPAATSLAFHPQDNNIIAIGMDDSSIQIYNVRVDEVKSK | ||||||
Repeat | 927-966 | WD 11 | ||||
Sequence: GHQKRVTGLAFSNVLNVLVSSGADSQLCVWSMDGWEKQAS | ||||||
Repeat | 1020-1059 | WD 12 | ||||
Sequence: ESSGSVTDAVYSCDSQSIYAAFDDGSVSILTATTLQLKCR | ||||||
Region | 1095-1135 | Disordered | ||||
Sequence: DGGVHVIEPPGPEGKWGISAPPENGAGPSVSSAPGSDQQPR | ||||||
Compositional bias | 1119-1135 | Polar residues | ||||
Sequence: GAGPSVSSAPGSDQQPR |
Domain
The N-terminal TOPLESS domain (TPD) (1-209) binds directly to a 12-amino acid LxLxL EAR motif peptide.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q27GK7-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length1,135
- Mass (Da)124,104
- Last updated2010-06-15 v2
- Checksum78F8FDFE56D7204F
Q27GK7-2
- Name2
- Differences from canonical
- 1135-1135: R → SDS
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4J043 | F4J043_ARATH | WSIP2 | 1125 |
Sequence caution
Features
Showing features for compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 286-302 | Polar residues | ||||
Sequence: PRSPPTNSLSMDYQTAD | ||||||
Compositional bias | 1119-1135 | Polar residues | ||||
Sequence: GAGPSVSSAPGSDQQPR | ||||||
Alternative sequence | VSP_039296 | 1135 | in isoform 2 | |||
Sequence: R → SDS |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB017071 EMBL· GenBank· DDBJ | BAB02318.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002686 EMBL· GenBank· DDBJ | AEE75740.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE75741.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | ANM63812.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY039963 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
AK229865 EMBL· GenBank· DDBJ | BAF01694.1 EMBL· GenBank· DDBJ | mRNA | ||
AK317630 EMBL· GenBank· DDBJ | BAH20292.1 EMBL· GenBank· DDBJ | mRNA |