Q27597 · NCPR_DROME
- ProteinNADPH--cytochrome P450 reductase
- GeneCpr
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids679 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
This enzyme is required for electron transfer from NADP to cytochrome p450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome b5 (By similarity).
May function to clear the olfactory organ (antennae) from accumulating chemicals
May function to clear the olfactory organ (antennae) from accumulating chemicals
Catalytic activity
- NADPH + 2 oxidized [cytochrome P450] = H+ + NADP+ + 2 reduced [cytochrome P450]
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 FAD per monomer.
Note: Binds 1 FMN per monomer.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 90-95 | FMN (UniProtKB | ChEBI) | ||||
Sequence: SQTGTG | ||||||
Binding site | 142-145 | FMN (UniProtKB | ChEBI) | ||||
Sequence: ATYG | ||||||
Binding site | 177-186 | FMN (UniProtKB | ChEBI) | ||||
Sequence: LGNKTYEHYN | ||||||
Binding site | 212 | FMN (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 302 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 458-461 | FAD (UniProtKB | ChEBI) | ||||
Sequence: RYYS | ||||||
Binding site | 476-478 | FAD (UniProtKB | ChEBI) | ||||
Sequence: TAV | ||||||
Binding site | 482 | FAD (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 492-495 | FAD (UniProtKB | ChEBI) | ||||
Sequence: GVAT | ||||||
Binding site | 537 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 597-598 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: SR | ||||||
Binding site | 603-607 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: KVYVQ | ||||||
Binding site | 640 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 678 | FAD (UniProtKB | ChEBI) | ||||
Sequence: W |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | endomembrane system | |
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | NADP binding | |
Molecular Function | NADPH-hemoprotein reductase activity | |
Biological Process | cuticle hydrocarbon biosynthetic process | |
Biological Process | response to hormone |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH--cytochrome P450 reductase
- EC number
- Short namesCPR ; P450R
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ27597
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-21 | Lumenal | ||||
Sequence: MASEQTIDGAAAIPSGGGDEP | ||||||
Transmembrane | 22-42 | Helical | ||||
Sequence: FLGLLDVALLAVLIGGAAFYF | ||||||
Topological domain | 43-679 | Cytoplasmic | ||||
Sequence: LRSRKKEEEPTRSYSIQPTTVCTTSASDNSFIKKLKASGRSLVVFYGSQTGTGEEFAGRLAKEGIRYRLKGMVADPEECDMEELLQLKDIDNSLAVFCLATYGEGDPTDNAMEFYEWITSGDVDLSGLNYAVFGLGNKTYEHYNKVAIYVDKRLEELGANRVFELGLGDDDANIEDDFITWKDRFWPAVCDHFGIEGGGEEVLIRQYRLLEQPDVQPDRIYTGEIARLHSIQNQRPPFDAKNPFLAPIKVNRELHKGGGRSCMHIELSIEGSKMRYDAGDHVAMFPVNDKSLVEKLGQLCNADLDTVFSLINTDTDSSKKHPFPCPTTYRTALTHYLEITAIPRTHILKELAEYCTDEKEKELLRSMASISPEGKEKYQSWIQDACRNIVHILEDIKSCRPPIDHVCELLPRLQPRYYSISSSAKLHPTDVHVTAVLVEYKTPTGRINKGVATTYLKNKQPQGSEEVKVPVFIRKSQFRLPTKPETPIIMVGPGTGLAPFRGFIQERQFLRDEGKTVGESILYFGCRKRSEDYIYESELEEWVKKGTLNLKAAFSRDQGKKVYVQHLLEQDADLIWNVIGENKGHFYICGDAKNMAVDVRNILVKILSTKGNMSEADAVQYIKKMEAQKRYSADVWS |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000167602 | 1-679 | NADPH--cytochrome P450 reductase | |||
Sequence: MASEQTIDGAAAIPSGGGDEPFLGLLDVALLAVLIGGAAFYFLRSRKKEEEPTRSYSIQPTTVCTTSASDNSFIKKLKASGRSLVVFYGSQTGTGEEFAGRLAKEGIRYRLKGMVADPEECDMEELLQLKDIDNSLAVFCLATYGEGDPTDNAMEFYEWITSGDVDLSGLNYAVFGLGNKTYEHYNKVAIYVDKRLEELGANRVFELGLGDDDANIEDDFITWKDRFWPAVCDHFGIEGGGEEVLIRQYRLLEQPDVQPDRIYTGEIARLHSIQNQRPPFDAKNPFLAPIKVNRELHKGGGRSCMHIELSIEGSKMRYDAGDHVAMFPVNDKSLVEKLGQLCNADLDTVFSLINTDTDSSKKHPFPCPTTYRTALTHYLEITAIPRTHILKELAEYCTDEKEKELLRSMASISPEGKEKYQSWIQDACRNIVHILEDIKSCRPPIDHVCELLPRLQPRYYSISSSAKLHPTDVHVTAVLVEYKTPTGRINKGVATTYLKNKQPQGSEEVKVPVFIRKSQFRLPTKPETPIIMVGPGTGLAPFRGFIQERQFLRDEGKTVGESILYFGCRKRSEDYIYESELEEWVKKGTLNLKAAFSRDQGKKVYVQHLLEQDADLIWNVIGENKGHFYICGDAKNMAVDVRNILVKILSTKGNMSEADAVQYIKKMEAQKRYSADVWS |
Proteomic databases
Expression
Tissue specificity
High in antennae.
Developmental stage
Embryos and adults.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 84-228 | Flavodoxin-like | ||||
Sequence: LVVFYGSQTGTGEEFAGRLAKEGIRYRLKGMVADPEECDMEELLQLKDIDNSLAVFCLATYGEGDPTDNAMEFYEWITSGDVDLSGLNYAVFGLGNKTYEHYNKVAIYVDKRLEELGANRVFELGLGDDDANIEDDFITWKDRFW | ||||||
Domain | 283-523 | FAD-binding FR-type | ||||
Sequence: KNPFLAPIKVNRELHKGGGRSCMHIELSIEGSKMRYDAGDHVAMFPVNDKSLVEKLGQLCNADLDTVFSLINTDTDSSKKHPFPCPTTYRTALTHYLEITAIPRTHILKELAEYCTDEKEKELLRSMASISPEGKEKYQSWIQDACRNIVHILEDIKSCRPPIDHVCELLPRLQPRYYSISSSAKLHPTDVHVTAVLVEYKTPTGRINKGVATTYLKNKQPQGSEEVKVPVFIRKSQFRLP |
Sequence similarities
Belongs to the NADPH--cytochrome P450 reductase family.
In the N-terminal section; belongs to the flavodoxin family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length679
- Mass (Da)76,347
- Last updated2000-12-01 v2
- ChecksumC6387C111A0EDB4A
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 38-39 | in Ref. 1; CAA63639 | ||||
Sequence: AA → VT | ||||||
Sequence conflict | 45 | in Ref. 1; CAA63639 | ||||
Sequence: S → T | ||||||
Sequence conflict | 132 | in Ref. 1; CAA63639 | ||||
Sequence: I → T |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X93090 EMBL· GenBank· DDBJ | CAA63639.1 EMBL· GenBank· DDBJ | mRNA | ||
AE014134 EMBL· GenBank· DDBJ | AAF52367.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY052000 EMBL· GenBank· DDBJ | AAK93424.1 EMBL· GenBank· DDBJ | mRNA |