Q27083 · CFGB_TACTR

Function

function

Component of the heterodimer clotting factor G which may play a role in defense mechanisms against fungi (Probable). Initiates a (1->3)-beta-glucan-sensing clotting pathway whereby the alpha subunit binds to glucans containing (1->3)-beta linkages, which are components of the fungal cell wall, and the beta subunit catalyzes the activation of proclotting enzyme (PubMed:7822328).

Catalytic activity

  • Selective cleavage of 98-Arg-|-Ile-99 bond in Limulus proclotting enzyme to form active clotting enzyme.
    EC:3.4.21.85 (UniProtKB | ENZYME | Rhea)

Activity regulation

Binding to (1->3)-beta-D-glucan to alpha subunit, induces autocatalysis and activation of beta subunit (PubMed:7822328).
Inhibited by intracellular coagulation inhibitor 3/LICI-3 and to a lesser extend by intracellular coagulation inhibitor 2/LICI-2 (PubMed:7822328, PubMed:8798603).

Biotechnology

Clotting factor G is a component of the Limulus test used to detect bacterial endotoxins in fluids.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site89Charge relay system
Active site138Charge relay system
Active site242Charge relay system

GO annotations

AspectTerm
Molecular Functionserine-type endopeptidase activity
Molecular Functionserine-type peptidase activity
Biological Processhemolymph coagulation
Biological Processprotein processing

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

Organism names

Accessions

  • Primary accession
    Q27083

Phenotypes & Variants

PTM/Processing

Features

Showing features for signal, chain, disulfide bond, glycosylation.

TypeIDPosition(s)Description
Signal1-31
ChainPRO_000045035632-46Clotting factor G beta subunit light chain
Disulfide bond38↔158
ChainPRO_500420347047-309Clotting factor G beta subunit heavy chain
Disulfide bond74↔90
Glycosylation100N-linked (GlcNAc...) asparagine
Disulfide bond205↔227
Glycosylation206N-linked (GlcNAc...) asparagine
Disulfide bond238↔268

Keywords

Expression

Tissue specificity

Expressed in the hemocytes (at protein level).

Interaction

Subunit

Clotting factor G is a heterodimer composed of two non-covalently associated subunits, alpha and beta (PubMed:7822328).
Upon activation, converted to a two-chain active form linked by a disulfide bond (PubMed:7822328).
Forms a covalent heterodimer with intracellular coagulation inhibitor 3/LICI-3 (PubMed:8798603).

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain47-292Peptidase S1

Sequence similarities

Belongs to the peptidase S1 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    309
  • Mass (Da)
    34,265
  • Last updated
    1996-11-01 v1
  • Checksum
    014F0B5F2BD56FDB
MDISFLVFITLSMALFSSNVTGTSVTSRVRRGINEKHCGFRPVITRIIGGGIATPHSWPWMVGIFKVNPHRFLCGGSIINKVSVVTAAHCLVTQFGNRQNYSIFVRVGAHDIDNSGTNYQVDKVIVHQGYKHHSHYYDIGLILLSKPVEYNDKIQPVCIPEFNKPHVNLNNIKVVITGWGVTGKATEKRNVLRELELPVVTNEQCNKSYQTLPFSKLNRGITNDMICAGFPEGGKDACQGDSGGPLMYQNPTTGRVKIVGVVSFGFECARPNFPGVYTRLSSYVNWLQEITFGQSLASLFEVVPIFIPE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D16623
EMBL· GenBank· DDBJ
BAA04045.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

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