Q25BN1 · DICER_CHICK
- ProteinEndoribonuclease Dicer
- GeneDICER1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1921 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes (By similarity).
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 2 magnesium or manganese ions per subunit.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 64-71 | ATP (UniProtKB | ChEBI) | ||||
Sequence: LNTGSGKT | ||||||
Binding site | 1317 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 1396 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 1399 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 1704 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Site | 1805 | Important for activity | ||||
Sequence: K | ||||||
Binding site | 1809 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 1812 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Cellular Component | RISC complex | |
Cellular Component | RISC-loading complex | |
Molecular Function | ATP binding | |
Molecular Function | deoxyribonuclease I activity | |
Molecular Function | helicase activity | |
Molecular Function | metal ion binding | |
Molecular Function | ribonuclease III activity | |
Molecular Function | RNA binding | |
Biological Process | apoptotic DNA fragmentation | |
Biological Process | global gene silencing by mRNA cleavage | |
Biological Process | pre-miRNA processing | |
Biological Process | siRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEndoribonuclease Dicer
- EC number
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Galloanserae > Galliformes > Phasianidae > Phasianinae > Gallus
Accessions
- Primary accessionQ25BN1
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000373984 | 1-1921 | Endoribonuclease Dicer | |||
Sequence: MKSPALQSLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNKNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSSLEVTESWTKEKWSQEFSKHQVLVMTCHVALTVLRNEYLSLSNINLLVFDECHLAIQDHPYREIMKICEDYPSCPRILGLTASILNGKCDPAELEEKIKKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPYTDKSGLYGRLLRELDEALHFLNDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTSETETEPIVDDDDVFPPYVLRTDENSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTRELPDHTFYSTLYLPINSPLRASIVGPPMSCARLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTEADSAYCVLPLNIVDDSSTLDIDFKFMEDIEKSEARTGIPSTQYTKEMPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTATDAGVGVKSLPADFRYPNLDFGWKKSIDSKSFISIPSSSLVENENYCKHSTIVVPENAAHQGANRTSSAEKHDQMSVSYRTLLDESPSKLQIDVSAELAAINGVSYNKNLANGNCDLVNRDFCQGNQLNYCRQEIPVQPTTSYPIQNLYSSENQPKPSNECTLLSNKYLDGNANRSTSDGCPKMTVTTSTSTALNLSKDKVDSEKNTSSGYSSKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYIVNQDKSNTDKWEKEETTKENLLANGKLDYDDDDEEDEDLMWRLPKEETDFEDDFLEYDQEHIKFIDSMLMGSGAFVKKISLSHFSTTDSNYEWKAPKKSSLGNVPFSSDFDDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDAGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKKTDWESTLCATGENCNSEQKNLSPNSVSACIVNSEPSLYKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFENFEKKINYSFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQMEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEMVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS |
Keywords
- PTM
Proteomic databases
Interaction
Subunit
Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, motif, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 51-227 | Helicase ATP-binding | ||||
Sequence: LLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNKNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSSLEVTESWTKEKWSQEFSKHQVLVMTCHVALTVLRNEYLSLSNINLLVFDECHLAIQDHPYREIMKICEDYPSCPRILGLTASILNGKCDPAELEEKIKKLE | ||||||
Motif | 175-178 | DECH box | ||||
Sequence: DECH | ||||||
Region | 409-433 | Disordered | ||||
Sequence: YVSWSDSEDDDEDEEIEEKEKPETN | ||||||
Compositional bias | 412-427 | Acidic residues | ||||
Sequence: WSDSEDDDEDEEIEEK | ||||||
Domain | 433-602 | Helicase C-terminal | ||||
Sequence: NFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTSETETE | ||||||
Domain | 630-722 | Dicer dsRNA-binding fold | ||||
Sequence: AIGHINRYCARLPSDPFTHLAPKCKTRELPDHTFYSTLYLPINSPLRASIVGPPMSCARLAERVVALICCEKLHKIGELDDHLMPVGKETVKY | ||||||
Region | 727-746 | Disordered | ||||
Sequence: DLHDEEETSVPGRPGSTKRR | ||||||
Domain | 895-1042 | PAZ | ||||
Sequence: KFMEDIEKSEARTGIPSTQYTKEMPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHP | ||||||
Region | 1270-1289 | Disordered | ||||
Sequence: NLSKDKVDSEKNTSSGYSSK | ||||||
Domain | 1277-1404 | RNase III 1 | ||||
Sequence: DSEKNTSSGYSSKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYIVNQDKSNTDKWEKEETT | ||||||
Domain | 1665-1823 | RNase III 2 | ||||
Sequence: FENFEKKINYSFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQMEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSG | ||||||
Region | 1782-1801 | Disordered | ||||
Sequence: QGMDSELRRSEEDEEKEEDI | ||||||
Domain | 1848-1913 | DRBM | ||||
Sequence: VPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKA |
Sequence similarities
Belongs to the helicase family. Dicer subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,921
- Mass (Da)218,610
- Last updated2009-09-01 v3
- ChecksumE1F9C9BE1D8CD611
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 412-427 | Acidic residues | ||||
Sequence: WSDSEDDDEDEEIEEK |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB253768 EMBL· GenBank· DDBJ | BAE87103.1 EMBL· GenBank· DDBJ | mRNA |