Q256B8 · PCKG_CHLFF
- ProteinPhosphoenolpyruvate carboxykinase [GTP]
- GenepckG
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids600 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Catalytic activity
- GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate
Cofactor
Note: Binds 1 Mn2+ ion per subunit.
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 76 | substrate | ||||
Sequence: R | ||||||
Binding site | 216-218 | substrate | ||||
Sequence: YGG | ||||||
Binding site | 225 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 245 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 267 | substrate | ||||
Sequence: S | ||||||
Binding site | 268-273 | GTP (UniProtKB | ChEBI) | ||||
Sequence: ACGKTN | ||||||
Active site | 269 | |||||
Sequence: C | ||||||
Binding site | 292 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 381-383 | substrate | ||||
Sequence: NSR | ||||||
Binding site | 383 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 414 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 508-511 | GTP (UniProtKB | ChEBI) | ||||
Sequence: FSEN |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | GTP binding | |
Molecular Function | manganese ion binding | |
Molecular Function | phosphoenolpyruvate carboxykinase (GTP) activity | |
Biological Process | gluconeogenesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate carboxykinase [GTP]
- EC number
- Short namesPEP carboxykinase ; PEPCK
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Chlamydiota > Chlamydiia > Chlamydiales > Chlamydiaceae > Chlamydia/Chlamydophila group > Chlamydia
Accessions
- Primary accessionQ256B8
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000080984 | 1-600 | Phosphoenolpyruvate carboxykinase [GTP] | |||
Sequence: MTSAWSNEIQHEGLKQWIQEVAELVTPEDIRVCNGSDSEYAEVYSIMQKSGVAIPLNSDLHPNCFLVRSSPEDVARVEKFTFICTAKKEEAGPTNNWRDPQEMRQELQGLFRGCMRGRTLYVVPFCMGPLNSPFSLIGVELTDSPYVVCSMKIMTRMGAEVLKSLGTSGTFHKCLHSVGVPLSPGEKDVAWPCNPEHMRIVHFQDDSSVMSFGSGYGGNALLGKKCVALRLASYIARSKNWLAEHMLIIGVTNPEGQKKYFAASFPSACGKTNLAMLKPKIPGWKIECIGDDIAWIRPGSDGRLYAVNPEYGFFGVAPGTSEKTNPNALATCRSNSIFTNVALTPDGDVWWEGLTSQPPAGLIDWRGNPWQPGGTPAAHPNSRFTTPLQQCPVLDSQWNSSEGVPLEAIIFGGRRSDTIPLVYEALSWQHGVTIGASMSSATTAAIVGEQGKLRHDPFAMLPFCGYNMALYFDHWLSFASNASLKLPKIYGVNWFRKDKDGNFIWPGFSENLRVLEWIFRRTNGEESIAKRTPIGYLPEESSLNLEGLNLSSQALQDLLAVDVPGWLKEVADVREYCKIFGSDLPQIISDELFRIERELK |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length600
- Mass (Da)66,477
- Last updated2006-04-18 v1
- Checksum52F3231C978B8B74
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP006861 EMBL· GenBank· DDBJ | BAE80870.1 EMBL· GenBank· DDBJ | Genomic DNA |