Q24XT2 · LEU3_DESHY

Function

function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mg2+ or Mn2+ ion per subunit.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site96substrate
Binding site106substrate
Binding site134substrate
Site141Important for catalysis
Site188Important for catalysis
Binding site220Mg2+ (UniProtKB | ChEBI)
Binding site220substrate
Binding site244Mg2+ (UniProtKB | ChEBI)
Binding site248Mg2+ (UniProtKB | ChEBI)
Binding site277-289NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3-isopropylmalate dehydrogenase activity
Molecular Functionmagnesium ion binding
Molecular FunctionNAD binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-isopropylmalate dehydrogenase
  • EC number
  • Alternative names
    • 3-IPM-DH
    • Beta-IPM dehydrogenase
      (IMDH
      )

Gene names

    • Name
      leuB
    • Ordered locus names
      DSY1371

Organism names

Accessions

  • Primary accession
    Q24XT2

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002501141-3523-isopropylmalate dehydrogenase

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    352
  • Mass (Da)
    38,029
  • Last updated
    2006-04-18 v1
  • Checksum
    852917EE5FA1DFD7
MPKIAVLPGDGIGQEIIPQAVRVLKAVLAETDAEFEFQDYPIGGAAIELVGKALPDETLQGCREADAVLLGAVGGHQWDHLPASERPETAALLGLRKGLNFYANIRPVRMIPSLLATSTLKENVLDGVDMVVIRELTGGVYFGEKGRSDNPRSAYDKMTYSEEEIRRILIQGFETAMLRSKKLCSVDKANVLETSRLWREIANELAKEYPEVELTHMYVDNAAMQLVRNPKQFDVIVTENMFGDILTDLASMLGGSIGMLSSASLSGTQGMYEPAHGSAPDIAGKNLANPLATILSAALMLRYSFGMEAEALRIESAVEKVLEQGYRTGDLAQAGDKVVGTIEMGDAVLAAL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP008230
EMBL· GenBank· DDBJ
BAE83160.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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