Q24QC9 · TSAD_DESHY
- ProteintRNA N6-adenosine threonylcarbamoyltransferase
- GenetsaD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids341 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.
Catalytic activity
- adenosine37 in tRNA + L-threonylcarbamoyladenylate = AMP + H+ + N6-L-threonylcarbamoyladenosine37 in tRNA
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 118 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 122 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 141-145 | substrate | ||||
Sequence: LVSGG | ||||||
Binding site | 174 | substrate | ||||
Sequence: D | ||||||
Binding site | 187 | substrate | ||||
Sequence: G | ||||||
Binding site | 281 | substrate | ||||
Sequence: N | ||||||
Binding site | 309 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | iron ion binding | |
Molecular Function | N(6)-L-threonylcarbamoyladenine synthase activity | |
Biological Process | tRNA threonylcarbamoyladenosine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA N6-adenosine threonylcarbamoyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Desulfitobacteriaceae > Desulfitobacterium
Accessions
- Primary accessionQ24QC9
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000303346 | 1-341 | tRNA N6-adenosine threonylcarbamoyltransferase | |||
Sequence: MPVNNKDINILGIETSCDETSAAVLRNGCELLSHIISSQIKTHQKYGGVVPEVASREHILHLQSVVEQALQEAKMGFGDLAGIAVTYGPGLVGSLLVGVAGAKAMAYGAGIPLLGVNHLEGHIYANFLHNPGLKFPLLALLVSGGHSHLVYFEGHGKYQVLGQTRDDAAGEALDKVARTLGLGYPGGPYIQKAALEGNPHAYEFPRAMLEPGSLDFSFSGVKSAVLNTLNSARMKGETVNVADVAASFQEAIVDVLVRKTLLALARTKAPTLVLAGGVAANTLLRERLTEATQKRGIAFSYPPPILCTDNGAMIATAGYYRYLDKDYAPWNLNAIPGLNLA |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length341
- Mass (Da)36,110
- Last updated2006-04-18 v1
- Checksum4F8DEA94A9C09A10
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP008230 EMBL· GenBank· DDBJ | BAE85763.1 EMBL· GenBank· DDBJ | Genomic DNA |