Q24478 · CP190_DROME
- ProteinCentrosome-associated zinc finger protein Cp190
- GeneCp190
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1096 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays a central role in chromatin domain organization and boundary function through recruitment by a range of insulator DNA-binding proteins, including ZIPIC, pita, CTCF, su(Hw) and others (PubMed:25342723, PubMed:33752739).
Together with pita and CTCF cooperatively binds to and regulates the activity of the Miscadastral pigmentation (MCP) insulator (PubMed:25342723).
Recruitment of Cp190 together with Chro/chromator induces chromatin decondensation (PubMed:33752739).
Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators (PubMed:15574329).
Chromatin insulators are regulatory elements that establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops to form topologically associating domains (TADs). This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may directly bind to insulator DNA at sites distinct from those recognized by su(Hw) (PubMed:15574329).
Required during embryogenesis for axial expansion, an actin/myosin dependent process that distributes the dividing nuclei along the anterior-posterior axis of the syncytial embryo (PubMed:16051175).
Localizes to centrosomes and recruits CP60 during mitosis but does not appear to play a crucial role in organizing centrosomal microtubules (PubMed:14996941, PubMed:16051175).
Together with pita and CTCF cooperatively binds to and regulates the activity of the Miscadastral pigmentation (MCP) insulator (PubMed:25342723).
Recruitment of Cp190 together with Chro/chromator induces chromatin decondensation (PubMed:33752739).
Component of the gypsy chromatin insulator complex which is required for the function of the gypsy chromatin insulator and other endogenous chromatin insulators (PubMed:15574329).
Chromatin insulators are regulatory elements that establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops to form topologically associating domains (TADs). This chromatin looping may involve the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may directly bind to insulator DNA at sites distinct from those recognized by su(Hw) (PubMed:15574329).
Required during embryogenesis for axial expansion, an actin/myosin dependent process that distributes the dividing nuclei along the anterior-posterior axis of the syncytial embryo (PubMed:16051175).
Localizes to centrosomes and recruits CP60 during mitosis but does not appear to play a crucial role in organizing centrosomal microtubules (PubMed:14996941, PubMed:16051175).
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCentrosome-associated zinc finger protein Cp190
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ24478
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes to nucleus during interphase and to the centrosome during mitosis. Colocalizes with other elements of the gypsy chromatin insulator complex at multiple sites on polytene chromosomes and at nuclear insulator bodies. On polytene chromosomes of third-instar larvae colocalizes with pita and ZIPIC (PubMed:25342723).
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000232629 | 1-1096 | Centrosome-associated zinc finger protein Cp190 | |||
Sequence: MGEVKSVKVDNWGVFFLQKLQNFFNKTDYCDLTLQFRDNSQLKVHRLVLSACTDYFNVLEQTCEIVDDALIMPNEFQADVVVPIVNFMYTGTLEFELKMYGKLLRTAKEMNMTVLLKLLEAHRRTMENVNRQQRPPSPKGIRRRTVGQPSSGLPQQRVLGPSPQSRNVATPIAQRANTQRGSTGNTMSRTSGGSNRSPYGDSSNVKQEPTSPFEQLRKGYNNNKRPAQTSLLSPPSKKPSLEEVKEFAEQQRMRKQIAAEYGDNDPEYDGGMLYDDVHAGDDDDDDMPPQPSTSKQQSPQGTQTQLEHGSTTIILKQDSPSQTPTIIVKDSSNAKLNHTKIIAEVLRQYPHIVKGHKNIKLKIMPNTPAAPTEKSAPATVKPPANQSSATTSPHKKLHVSFKADKSTPLITAQQKAASSQQKSGTSQTTGNQGTGANPPANTAAAQKRRIDSKTMHALIAQGAENTTGPWLCLRCGVNGRPISIPSYRGFRRHLINTHKETIDPALCEHCGWRSVNNRELHFHMYMEHQTKSLLYTFAECALCNQSYRTKGELEAHINEVHTDDNKQQCIYCNKVFEQELQLYRHMKSYHKEQALEDGIIDETDEEFLGSQDEEEEAEGDEEQEPEQTGKVRILSDISLPATSAITVQQAQQEQLQEEDVEQVQQEVKFVGADGNEVELTDEQRKEILSQLNQQQAGATAGGVVMVLSEPEAEHVKQETDEKSLAGTEEEYDDSQIYSELGAADSVESAKKNIADESKESIDNLEWAENLIAESEEQSNKEPKSDKPRDDISEKLKELTGDWTEDENDDDVDDKPATAELASELANKDPEPTVHEEEDDIDLALQSLHKGPEEATEEKASEESVTSADDAVDAVPNINSQPEKMDVDSEAADEKASKAEVQIKKEAELENDQEEFIKEDSPIPHSDSVAELREAVTASEGEDDVHLEADNIRKELLDELIAEAEKPDQEKDIVQSEENATTEALDRSVTDEDDLVPPTQVSTEQMEIDEPAAEKAAENNEDTRTADEKEAVEDKPNQTQDVTTAEKPTLESAKAGDEATSGEAASVDKVKSLISEWGDDDEDEDENGVSAAAKEEL | ||||||
Modified residue | 197 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 211 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 229 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 233 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 298 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 319 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 603 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 610 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 708 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 723 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 727 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 745 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 748 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 757 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 760 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 817 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 920 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 925 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 927 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 936 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 938 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 1071 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 1074 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in spermatids but not in mature spermatozoa. Localizes within the spermatids to a sheath of microtubules around the nucleus and to microtubules within the tail.
Developmental stage
Expressed in 0-12 hour old embryos and 3rd instar larvae (at protein level) (PubMed:25342723).
Localizes to the centrosome throughout the nuclear division cycle in early syncytial embryos. Localization to the interphase nucleus is seen from nuclear cycle 9 onwards
Localizes to the centrosome throughout the nuclear division cycle in early syncytial embryos. Localization to the interphase nucleus is seen from nuclear cycle 9 onwards
Gene expression databases
Interaction
Subunit
Component of the gypsy chromatin insulator complex, composed of Cp190, mod(mdg4) and su(Hw) (PubMed:15574329).
The gypsy chromatin insulator complex interacts with Topors via mod(mdg4) and su(Hw) (PubMed:15574329).
Interacts with Cp60 and microtubules (PubMed:14996941, PubMed:8491775, PubMed:8522588, PubMed:8590797, PubMed:9700165).
Interacts with inv (PubMed:25561495).
Interacts with Nup98 (PubMed:28366641).
Interacts (via BTB domain) with pita (via region between the ZAD domain and the first zinc finger domain); the interaction is direct (PubMed:25342723, PubMed:33752739).
Interacts with ZIPIC (via region between the ZAD domain and the first Zinc finger domain); the interaction is direct (PubMed:25342723).
The gypsy chromatin insulator complex interacts with Topors via mod(mdg4) and su(Hw) (PubMed:15574329).
Interacts with Cp60 and microtubules (PubMed:14996941, PubMed:8491775, PubMed:8522588, PubMed:8590797, PubMed:9700165).
Interacts with inv (PubMed:25561495).
Interacts with Nup98 (PubMed:28366641).
Interacts (via BTB domain) with pita (via region between the ZAD domain and the first zinc finger domain); the interaction is direct (PubMed:25342723, PubMed:33752739).
Interacts with ZIPIC (via region between the ZAD domain and the first Zinc finger domain); the interaction is direct (PubMed:25342723).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q24478 | CG4639 Q8T051 | 7 | EBI-868840, EBI-192200 | |
BINARY | Q24478 | Cp190 Q24478 | 3 | EBI-868840, EBI-868840 | |
BINARY | Q24478 | CTCF Q8TA44 | 5 | EBI-868840, EBI-466743 | |
BINARY | Q24478 | CTCF Q9VS55 | 5 | EBI-868840, EBI-149931 | |
BINARY | Q24478 | Dmel\CG4730 Q9VBN9 | 10 | EBI-868840, EBI-127047 | |
BINARY | Q24478 | Ibf1 Q9VHG5 | 3 | EBI-868840, EBI-141691 | |
BINARY | Q24478 | Ibf2 Q9VHG6 | 3 | EBI-868840, EBI-157022 | |
BINARY | Q24478 | mod(mdg4) Q86B87-1 | 4 | EBI-868840, EBI-1433422 | |
BINARY | Q24478 | pita Q95RQ8 | 8 | EBI-868840, EBI-114363 | |
BINARY | Q24478 | su(Hw) P08970 | 12 | EBI-868840, EBI-101373 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias, zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 30-97 | BTB | ||||
Sequence: CDLTLQFRDNSQLKVHRLVLSACTDYFNVLEQTCEIVDDALIMPNEFQADVVVPIVNFMYTGTLEFEL | ||||||
Region | 126-308 | Disordered | ||||
Sequence: MENVNRQQRPPSPKGIRRRTVGQPSSGLPQQRVLGPSPQSRNVATPIAQRANTQRGSTGNTMSRTSGGSNRSPYGDSSNVKQEPTSPFEQLRKGYNNNKRPAQTSLLSPPSKKPSLEEVKEFAEQQRMRKQIAAEYGDNDPEYDGGMLYDDVHAGDDDDDDMPPQPSTSKQQSPQGTQTQLEH | ||||||
Compositional bias | 148-236 | Polar residues | ||||
Sequence: QPSSGLPQQRVLGPSPQSRNVATPIAQRANTQRGSTGNTMSRTSGGSNRSPYGDSSNVKQEPTSPFEQLRKGYNNNKRPAQTSLLSPPS | ||||||
Region | 207-271 | Nuclear localization | ||||
Sequence: QEPTSPFEQLRKGYNNNKRPAQTSLLSPPSKKPSLEEVKEFAEQQRMRKQIAAEYGDNDPEYDGG | ||||||
Compositional bias | 239-262 | Basic and acidic residues | ||||
Sequence: PSLEEVKEFAEQQRMRKQIAAEYG | ||||||
Region | 245-468 | Centrosomal targeting M domain involved in interaction with ZIPIC | ||||
Sequence: KEFAEQQRMRKQIAAEYGDNDPEYDGGMLYDDVHAGDDDDDDMPPQPSTSKQQSPQGTQTQLEHGSTTIILKQDSPSQTPTIIVKDSSNAKLNHTKIIAEVLRQYPHIVKGHKNIKLKIMPNTPAAPTEKSAPATVKPPANQSSATTSPHKKLHVSFKADKSTPLITAQQKAASSQQKSGTSQTTGNQGTGANPPANTAAAQKRRIDSKTMHALIAQGAENTTG | ||||||
Compositional bias | 290-308 | Polar residues | ||||
Sequence: QPSTSKQQSPQGTQTQLEH | ||||||
Region | 366-449 | Disordered | ||||
Sequence: NTPAAPTEKSAPATVKPPANQSSATTSPHKKLHVSFKADKSTPLITAQQKAASSQQKSGTSQTTGNQGTGANPPANTAAAQKRR | ||||||
Compositional bias | 379-395 | Polar residues | ||||
Sequence: TVKPPANQSSATTSPHK | ||||||
Region | 385-508 | Centrosomal localization and interaction with microtubules | ||||
Sequence: NQSSATTSPHKKLHVSFKADKSTPLITAQQKAASSQQKSGTSQTTGNQGTGANPPANTAAAQKRRIDSKTMHALIAQGAENTTGPWLCLRCGVNGRPISIPSYRGFRRHLINTHKETIDPALCE | ||||||
Compositional bias | 406-441 | Polar residues | ||||
Sequence: STPLITAQQKAASSQQKSGTSQTTGNQGTGANPPAN | ||||||
Zinc finger | 538-561 | C2H2-type 1 | ||||
Sequence: AECALCNQSYRTKGELEAHINEVH | ||||||
Zinc finger | 567-590 | C2H2-type 2 | ||||
Sequence: QQCIYCNKVFEQELQLYRHMKSYH | ||||||
Compositional bias | 608-623 | Acidic residues | ||||
Sequence: LGSQDEEEEAEGDEEQ | ||||||
Region | 608-630 | Disordered | ||||
Sequence: LGSQDEEEEAEGDEEQEPEQTGK | ||||||
Region | 710-733 | Disordered | ||||
Sequence: PEAEHVKQETDEKSLAGTEEEYDD | ||||||
Region | 770-927 | Disordered | ||||
Sequence: LIAESEEQSNKEPKSDKPRDDISEKLKELTGDWTEDENDDDVDDKPATAELASELANKDPEPTVHEEEDDIDLALQSLHKGPEEATEEKASEESVTSADDAVDAVPNINSQPEKMDVDSEAADEKASKAEVQIKKEAELENDQEEFIKEDSPIPHSDS | ||||||
Compositional bias | 774-801 | Basic and acidic residues | ||||
Sequence: SEEQSNKEPKSDKPRDDISEKLKELTGD | ||||||
Compositional bias | 828-865 | Basic and acidic residues | ||||
Sequence: DPEPTVHEEEDDIDLALQSLHKGPEEATEEKASEESVT | ||||||
Compositional bias | 885-926 | Basic and acidic residues | ||||
Sequence: DVDSEAADEKASKAEVQIKKEAELENDQEEFIKEDSPIPHSD | ||||||
Compositional bias | 960-976 | Basic and acidic residues | ||||
Sequence: IAEAEKPDQEKDIVQSE | ||||||
Region | 960-1096 | Disordered | ||||
Sequence: IAEAEKPDQEKDIVQSEENATTEALDRSVTDEDDLVPPTQVSTEQMEIDEPAAEKAAENNEDTRTADEKEAVEDKPNQTQDVTTAEKPTLESAKAGDEATSGEAASVDKVKSLISEWGDDDEDEDENGVSAAAKEEL | ||||||
Compositional bias | 1007-1035 | Basic and acidic residues | ||||
Sequence: IDEPAAEKAAENNEDTRTADEKEAVEDKP |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,096
- Mass (Da)121,679
- Last updated2006-04-18 v2
- Checksum5CD5C5492B948D39
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 148-236 | Polar residues | ||||
Sequence: QPSSGLPQQRVLGPSPQSRNVATPIAQRANTQRGSTGNTMSRTSGGSNRSPYGDSSNVKQEPTSPFEQLRKGYNNNKRPAQTSLLSPPS | ||||||
Compositional bias | 239-262 | Basic and acidic residues | ||||
Sequence: PSLEEVKEFAEQQRMRKQIAAEYG | ||||||
Compositional bias | 290-308 | Polar residues | ||||
Sequence: QPSTSKQQSPQGTQTQLEH | ||||||
Compositional bias | 379-395 | Polar residues | ||||
Sequence: TVKPPANQSSATTSPHK | ||||||
Compositional bias | 406-441 | Polar residues | ||||
Sequence: STPLITAQQKAASSQQKSGTSQTTGNQGTGANPPAN | ||||||
Compositional bias | 608-623 | Acidic residues | ||||
Sequence: LGSQDEEEEAEGDEEQ | ||||||
Compositional bias | 774-801 | Basic and acidic residues | ||||
Sequence: SEEQSNKEPKSDKPRDDISEKLKELTGD | ||||||
Compositional bias | 828-865 | Basic and acidic residues | ||||
Sequence: DPEPTVHEEEDDIDLALQSLHKGPEEATEEKASEESVT | ||||||
Compositional bias | 885-926 | Basic and acidic residues | ||||
Sequence: DVDSEAADEKASKAEVQIKKEAELENDQEEFIKEDSPIPHSD | ||||||
Compositional bias | 960-976 | Basic and acidic residues | ||||
Sequence: IAEAEKPDQEKDIVQSE | ||||||
Compositional bias | 1007-1035 | Basic and acidic residues | ||||
Sequence: IDEPAAEKAAENNEDTRTADEKEAVEDKP | ||||||
Sequence conflict | 1061 | in Ref. 1; CAA90324 | ||||
Sequence: G → D |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z50021 EMBL· GenBank· DDBJ | CAA90324.1 EMBL· GenBank· DDBJ | mRNA | ||
AE014297 EMBL· GenBank· DDBJ | AAF55159.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014297 EMBL· GenBank· DDBJ | AAN13643.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT010090 EMBL· GenBank· DDBJ | AAQ22559.1 EMBL· GenBank· DDBJ | mRNA |