Q24311 · CUL1_DROME

Function

function

Core component of multiple SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. During early metamorphosis, part of the SCF-slmb complex that negatively regulates the InR/PI3K/TOR pathway to activate the pruning of unnecessary larval ddaC dendrites and mushroom body axons (PubMed:24068890).
The SCF-slmb complex also regulates asymmetrical division of neuroblasts and inhibits ectopic neuroblast formation partly through SAK and Akt1 (PubMed:24413555).
Also part of an SCF complex required for caspase activation during sperm differentiation (PubMed:20392747).
Necessary for auditory transduction: plays a role in Johnston's organ organization by acting in the regulation of zip and ck function in scolopidial apical attachment (PubMed:27331610).
May function by acting in a Ubr3-mediated pathway that negatively regulates the ubiquitination of zip, consequently affecting its interaction with ck (PubMed:27331610).

Pathway

Protein modification; protein ubiquitination.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytosol
Cellular Componentnucleus
Cellular ComponentSCF ubiquitin ligase complex
Molecular Functionprotein-macromolecule adaptor activity
Molecular Functionubiquitin ligase complex scaffold activity
Molecular Functionubiquitin protein ligase binding
Biological Processnegative regulation of antimicrobial peptide production
Biological Processnegative regulation of apoptotic process
Biological Processnegative regulation of canonical Wnt signaling pathway
Biological Processnegative regulation of hippo signaling
Biological Processnegative regulation of insulin receptor signaling pathway
Biological Processnegative regulation of peptidoglycan recognition protein signaling pathway
Biological Processnegative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
Biological Processnegative regulation of smoothened signaling pathway
Biological Processpositive regulation of neuron remodeling
Biological Processpositive regulation of proteasomal ubiquitin-dependent protein catabolic process
Biological Processpositive regulation of protein ubiquitination
Biological Processpositive regulation of synaptonemal complex assembly
Biological Processprotein catabolic process
Biological Processprotein K11-linked ubiquitination
Biological Processprotein ubiquitination
Biological ProcessSCF-dependent proteasomal ubiquitin-dependent protein catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cullin homolog 1
  • Alternative names
    • Lin-19 homolog protein

Gene names

    • Name
      Cul1
    • Synonyms
      cul-1, lin19
    • ORF names
      CG1877

Organism names

  • Taxonomic identifier
  • Strains
    • Oregon-R
    • Berkeley
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    Q24311
  • Secondary accessions
    • A4UZ78
    • Q0E9G0
    • Q9V312

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Note: In the spermatid, colocalizes with ntc at the actin-based individualization complex and the cystic bulge.

Keywords

Phenotypes & Variants

Disruption phenotype

Severe dendrite pruning defects in ddaC neurons at 16 hours after puparium formation (APF) and axon pruning defects in mushroom body gamma neurons at 24 hours APF. RNAi-mediated knockdown results in a significant increase in Akt1 protein levels and activity in ddaC somas (PubMed:24068890).
In the larval brain there is a large increase in the number of neuroblasts, 40% of which show a spindle misorientation at metaphase (PubMed:24413555).
RNAi-mediated knockdown results in apical detachment of scolopidial cells in Johnston's organ (PubMed:27331610).

PTM/Processing

Features

Showing features for chain, cross-link.

TypeIDPosition(s)Description
ChainPRO_00001197861-774Cullin homolog 1
Cross-link718Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)

Post-translational modification

Neddylated (PubMed:12183637, PubMed:18493598).
Deneddylated via its interaction with the COP9 signalosome (CSN) complex (PubMed:12183637).

Keywords

Proteomic databases

Expression

Tissue specificity

Expressed in testis.

Gene expression databases

Interaction

Subunit

Component of SCF E3 ubiquitin-protein ligase complexes consisting of Skpa, Cul1, Roc1a and an F-box protein. In larval neuroblast self renewal and asymmetric division, as well as ddaC dendrite and mushroom body axon pruning, the complex contains the F-box protein slmb (SCF-slmb) (PubMed:24068890, PubMed:24413555).
In caspase activation during sperm differentiation, the complex contains the F-box protein ntc (PubMed:20392747).
Interacts directly with Roc1a (PubMed:11500045).
Interacts with Fsn (PubMed:20123973).
Interacts with Dlish (PubMed:27692068).
Interacts with Cad99C (via the cytoplasmic domain) (PubMed:27331610).
Interacts with Sans (PubMed:27331610).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q24311Rca1 P916662EBI-136038, EBI-7085629
BINARY Q24311SkpA O774302EBI-136038, EBI-180180
View interactors in UniProtKB
View CPX-2646 in Complex Portal

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cullin family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    774
  • Mass (Da)
    89,511
  • Last updated
    2001-11-02 v2
  • Checksum
    1AF0D035DE85363C
MNRSGNSQTTQKLVNLDDIWSELVEGIMQVFEHEKSLTRSQYMRFYTHVYDYCTSVSAAPSGRSSGKTGGAQLVGKKLYDRLEQFLKSYLSELLTKFKAISGEEVLLSRYTKQWKSYQFSSTVLDGICNYLNRNWVKRECEEGQKGIYKIYRLALVAWKGHLFQVLNEPVTKAVLKSIEEERQGKLINRSLVRDVIECYVELSFNEEDTDAEQQKLSVYKQNFENKFIADTSAFYEKESDAFLSTNTVTEYLKHVENRLEEETQRVRGFNSKNGLSYLHETTADVLKSTCEEVLIEKHLKIFHTEFQNLLNADRNDDLKRMYSLVALSSKNLTDLKSILENHILHQGTEAIAKCCTTDAANDPKTYVQTILDVHKKYNALVLTAFNNDNGFVAALDKACGKFINSNVVTIANSASKSPELLAKYCDLLLKKSSKNPEDKELEDNLNQVMVVFKYIEDKDVFQKYYSKMLAKRLVNHTSASDDAEAMMISKLKQTCGYEYTVKLQRMFQDIGVSKDLNSYFKQYLAEKNLTMEIDFGIEVLSSGSWPFQLSNNFLLPSELERSVRQFNEFYAARHSGRKLNWLYQMCKGELIMNVNRNNSSTYTLQASTFQMSVLLQFNDQLSFTVQQLQDNTQTQQENLIQVLQILLKAKVLTSSDNENSLTPESTVELFLDYKNKKRRININQPLKTELKVEQETVHKHIEEDRKLLIQAAIVRIMKMRKRLNHTNLISEVLNQLSTRFKPKVPVIKKCIDILIEKEYLERMEGHKDTYSYLA

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict266in Ref. 1; AAA85085

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L41642
EMBL· GenBank· DDBJ
AAA85085.1
EMBL· GenBank· DDBJ
mRNA
AF136343
EMBL· GenBank· DDBJ
AAD33676.1
EMBL· GenBank· DDBJ
mRNA
AE013599
EMBL· GenBank· DDBJ
AAF59174.1
EMBL· GenBank· DDBJ
Genomic DNA
AE013599
EMBL· GenBank· DDBJ
AAM68871.1
EMBL· GenBank· DDBJ
Genomic DNA
AE013599
EMBL· GenBank· DDBJ
AAM68872.1
EMBL· GenBank· DDBJ
Genomic DNA
BT010290
EMBL· GenBank· DDBJ
AAQ23608.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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