Q22592 · BECN_CAEEL
- ProteinBeclin homolog
- Genebec-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids375 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Regulates autophagy (PubMed:12958363).
Together with phosphatidyl-3-phosphate kinase vps-34, acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate (PtdIns3P), thereby regulating membrane trafficking (PubMed:16111945, PubMed:21183797, PubMed:26783301).
In association with sorf-1 and sorf-2, negatively regulates phosphatidylinositol 3-phosphate in early endosomes to allow for the conversion to late endosomes (PubMed:26783301).
Involved in the clearance of engulfed apoptotic cell corpses (PubMed:16111945, PubMed:21183797).
Together with ced-9, negatively regulates somatic and germline apoptosis (PubMed:17890369).
Plays a role in endosome-to-Golgi retrograde transport of mig-14 (PubMed:21183797).
In a daf-18/PTEN- and skn-1/Nrf-dependent manner, promotes germline stem cell proliferation during late and adult stages, probably by ensuring cell survival and cell cycle progression (PubMed:28285998).
Required for embryonic development and L3/L4 molting during larval development (PubMed:16111945).
Required for normal dauer morphogenesis and lifespan (PubMed:12958363, PubMed:17204841).
Plays a role in male tail ray pattern formation (PubMed:17890369).
Required for normal survival when exposed to pathogenic bacteria S.typhimurium by promoting autophagic degradation of intracellular S.typhimurium (PubMed:19667176).
Together with phosphatidyl-3-phosphate kinase vps-34, acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate (PtdIns3P), thereby regulating membrane trafficking (PubMed:16111945, PubMed:21183797, PubMed:26783301).
In association with sorf-1 and sorf-2, negatively regulates phosphatidylinositol 3-phosphate in early endosomes to allow for the conversion to late endosomes (PubMed:26783301).
Involved in the clearance of engulfed apoptotic cell corpses (PubMed:16111945, PubMed:21183797).
Together with ced-9, negatively regulates somatic and germline apoptosis (PubMed:17890369).
Plays a role in endosome-to-Golgi retrograde transport of mig-14 (PubMed:21183797).
In a daf-18/PTEN- and skn-1/Nrf-dependent manner, promotes germline stem cell proliferation during late and adult stages, probably by ensuring cell survival and cell cycle progression (PubMed:28285998).
Required for embryonic development and L3/L4 molting during larval development (PubMed:16111945).
Required for normal dauer morphogenesis and lifespan (PubMed:12958363, PubMed:17204841).
Plays a role in male tail ray pattern formation (PubMed:17890369).
Required for normal survival when exposed to pathogenic bacteria S.typhimurium by promoting autophagic degradation of intracellular S.typhimurium (PubMed:19667176).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBeclin homolog
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ22592
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Co-localizes with rme-8, a component of the retromer complex, on endosomes.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Embryonic lethal with few surviving larvae which fail to carry out the L3/L4 molt and few surviving adults which are sterile (PubMed:16111945, PubMed:21183797).
At the L3 larval stage, accumulates vacuoles in intestine, hypodermis, pharynx and coelomocytes (PubMed:21183797).
Abnormal accumulation of mig-14, lmp-1, and rab-7 on these vacuoles (PubMed:21183797).
Mislocalization of several components of the retromer complex, including rme-8, snx-1 and vps-35 (PubMed:21183797).
Impaired fluid phase endocytosis in coelomocytes (PubMed:21183797).
Double knockout with either sorf-1 or sorf-2 results in smaller endosomes and an irregular distribution pattern of PtdIns3P in the cytoplasm (PubMed:26783301).
RNAi-mediated knockdown results in reduced stem cell proliferation in the germline during larval development (PubMed:28285998).
RNAi-mediated knockdown at the L4 larval stage causes a slight decrease in lifespan and multiple defects in the progeny which fails to produce viable embryos (PubMed:12958363, PubMed:16111945, PubMed:17204841).
In addition, causes an increase in the number of apoptotic cell corpses at various embryonic stages and in the gonads (PubMed:16111945, PubMed:21183797).
Reduces phosphatidylinositol 3-phosphate levels on intracellular membrane vesicles and impairs endocytosis (PubMed:16111945, PubMed:21183797).
Prevents necrotic cell death of CEP and ADE dopamine neurons induced by neurotoxin 6-hydroxidopamine (PubMed:17327275).
Impaired survival when exposed to pathogenic bacteria S.typhimurium which is associated with the persistence of intact bacteria-containing vesicles in intestinal cells, the formation of few autophagosomes and autolysosomes followed by a progressive destruction of intestinal cells (PubMed:19667176).
In addition, causes a reduction in mig-14 and rme-8 association with puncta structures as well as an increase in mig-14 protein levels (PubMed:21183797).
RNAi-mediated knockdown causes abnormalities in constitutive dauer formation in daf-2 e1370 mutant including uneven distribution of hyperpigmented granules in the intestine and a reduction in fat storage and radial constriction elongation of the body and the pharynx (PubMed:12958363).
In addition, prevents increase in lifespan and the formation of autophagosomes in lateral hypodermal seam cells in daf-2 e1370 mutant (PubMed:12958363, PubMed:19667176).
Reduces the number of vacuolated (dying) touch receptor neurons and restores touch sensitivity in mec-4 u231, deg-1 u506 or deg-3 u662 mutants (PubMed:17327275).
At the L3 larval stage, accumulates vacuoles in intestine, hypodermis, pharynx and coelomocytes (PubMed:21183797).
Abnormal accumulation of mig-14, lmp-1, and rab-7 on these vacuoles (PubMed:21183797).
Mislocalization of several components of the retromer complex, including rme-8, snx-1 and vps-35 (PubMed:21183797).
Impaired fluid phase endocytosis in coelomocytes (PubMed:21183797).
Double knockout with either sorf-1 or sorf-2 results in smaller endosomes and an irregular distribution pattern of PtdIns3P in the cytoplasm (PubMed:26783301).
RNAi-mediated knockdown results in reduced stem cell proliferation in the germline during larval development (PubMed:28285998).
RNAi-mediated knockdown at the L4 larval stage causes a slight decrease in lifespan and multiple defects in the progeny which fails to produce viable embryos (PubMed:12958363, PubMed:16111945, PubMed:17204841).
In addition, causes an increase in the number of apoptotic cell corpses at various embryonic stages and in the gonads (PubMed:16111945, PubMed:21183797).
Reduces phosphatidylinositol 3-phosphate levels on intracellular membrane vesicles and impairs endocytosis (PubMed:16111945, PubMed:21183797).
Prevents necrotic cell death of CEP and ADE dopamine neurons induced by neurotoxin 6-hydroxidopamine (PubMed:17327275).
Impaired survival when exposed to pathogenic bacteria S.typhimurium which is associated with the persistence of intact bacteria-containing vesicles in intestinal cells, the formation of few autophagosomes and autolysosomes followed by a progressive destruction of intestinal cells (PubMed:19667176).
In addition, causes a reduction in mig-14 and rme-8 association with puncta structures as well as an increase in mig-14 protein levels (PubMed:21183797).
RNAi-mediated knockdown causes abnormalities in constitutive dauer formation in daf-2 e1370 mutant including uneven distribution of hyperpigmented granules in the intestine and a reduction in fat storage and radial constriction elongation of the body and the pharynx (PubMed:12958363).
In addition, prevents increase in lifespan and the formation of autophagosomes in lateral hypodermal seam cells in daf-2 e1370 mutant (PubMed:12958363, PubMed:19667176).
Reduces the number of vacuolated (dying) touch receptor neurons and restores touch sensitivity in mec-4 u231, deg-1 u506 or deg-3 u662 mutants (PubMed:17327275).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000436304 | 1-375 | Beclin homolog | |||
Sequence: MTTQRSHICLNCQHPLRLDFTQRRPDSADSEKKSETVITEALTGHSRNLMKLISDAQFPSDAPVCNDCSDALRNEMDAQVATLDDEIKTYQTYINYLKENHPTTSIPDLKAKLQNVSDEEKELEQQLKKLLAEEEQLDLDLQTKRRTAEAASEKSGELWKKYRDNLRQVFEDQDELHSLEAERQYAEVQHRKLTDTNVLDLCFHIWVDGIVGEINGFRLGYLKDAPVEFTEINAALGQIVLLLEILLERIGVQHHELMPVAMGSHSYIKLRRNGIDMETYALYGQGTPLSGSSGIDPGIRRFLQLLEFLLKELKDRNKNFKPPYQIHADSLVDNGVKYNAVMTLNTDVRWTRAMALMLTDLKAACAQCDALRSPI |
Proteomic databases
Expression
Tissue specificity
Expressed in PVM touch receptor neurons, the ventral nerve cord and in the nerve ring.
Developmental stage
Highly expressed in embryos and to a lesser extent in larvae (PubMed:16111945).
Expressed in pharynx, seam cells and ventral nerve cord neurons at the L1 and L4 larval stages (PubMed:12958363, PubMed:16111945).
Expressed in vulva cell precursors and somatic gonad at the L2 larval stage and in vulva at the L4 larval stage (PubMed:12958363).
Expressed in pharynx, seam cells and ventral nerve cord neurons at the L1 and L4 larval stages (PubMed:12958363, PubMed:16111945).
Expressed in vulva cell precursors and somatic gonad at the L2 larval stage and in vulva at the L4 larval stage (PubMed:12958363).
Gene expression databases
Interaction
Subunit
Interacts with ced-9 (PubMed:16111945).
Interacts with vps-34 (PubMed:16111945, PubMed:26783301).
Interacts with sorf-1 and sorf-2 (PubMed:26783301).
Interacts with vps-34 (PubMed:16111945, PubMed:26783301).
Interacts with sorf-1 and sorf-2 (PubMed:26783301).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q22592 | sorf-1 Q23533 | 3 | EBI-2413500, EBI-13941886 | |
BINARY | Q22592 | sorf-2 Q10122 | 3 | EBI-2413500, EBI-13941960 | |
BINARY | Q22592 | vps-34 Q9TXI7 | 2 | EBI-2413500, EBI-21398789 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Coiled coil | 71-147 | |||||
Sequence: ALRNEMDAQVATLDDEIKTYQTYINYLKENHPTTSIPDLKAKLQNVSDEEKELEQQLKKLLAEEEQLDLDLQTKRRT |
Sequence similarities
Belongs to the beclin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q22592-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length375
- Mass (Da)42,774
- Last updated2001-10-01 v2
- Checksum630D0F04EEF324D8
Q22592-2
- Nameb
- Differences from canonical
- 1-257: Missing
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_058342 | 1-257 | in isoform b | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX284604 EMBL· GenBank· DDBJ | CCD62215.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284604 EMBL· GenBank· DDBJ | CDK13324.1 EMBL· GenBank· DDBJ | Genomic DNA |