Q214L6 · RIMO_RHOPB
- ProteinRibosomal protein uS12 methylthiotransferase RimO
- GenerimO
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids441 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein uS12.
Catalytic activity
- [sulfur carrier]-SH + AH2 + L-aspartate89-[ribosomal protein uS12]-hydrogen + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L-aspartate89-[ribosomal protein uS12]-hydrogen + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H+ + L-methionine + S-adenosyl-L-homocysteine
RHEA-COMP:14737 + CHEBI:17499 + RHEA-COMP:10461 CHEBI:29961 Position: 89CHEBI:64428 + 2 CHEBI:59789 = RHEA-COMP:10460 + CHEBI:17319 + RHEA-COMP:14739 + CHEBI:13193 + 2 CHEBI:15378 + CHEBI:57844 + CHEBI:57856
Cofactor
Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 16 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 52 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 81 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 148 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 152 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 155 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | protein methylthiotransferase activity | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibosomal protein uS12 methylthiotransferase RimO
- EC number
- Short namesuS12 MTTase ; uS12 methylthiotransferase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Nitrobacteraceae > Rhodopseudomonas
Accessions
- Primary accessionQ214L6
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000374972 | 1-441 | Ribosomal protein uS12 methylthiotransferase RimO | |||
Sequence: MNQAAAPKVSFVSLGCPKALVDSERIITRLRAEGYELARKHDGADLVIVNTCGFLDSAKKESLGAIGEAMAANGKVIVTGCMGAEPEQIEAAYPNLLSITGPQQYESVLDAVHRALPPLHNPHLDLVPAQGIKLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLVSRPAGEVLREAEKLVKAGVKELLVVSQDTSAYGVDLKYAESPWQDRNVRARFYDLAKELGELGAWVRLQYVYPYPHVDEVIGLMAQGKVLPYLDIPFQHASPEVLKQMKRPAAQDKTLARIKQWREICPELTLRSTFIVGFPGETDSDFAYLLDWLEQAEIDRVGAFKYEAVRGATSNALPDQVSDEVKTERWNALMARQQKISARRLKRKVGTRQQVIIDEVGPTVSKGRSKADAPQIDGSVYLTSRRPLRVGEIVTAKIERADAYDLHGSVAGF |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 7-117 | MTTase N-terminal | ||||
Sequence: PKVSFVSLGCPKALVDSERIITRLRAEGYELARKHDGADLVIVNTCGFLDSAKKESLGAIGEAMAANGKVIVTGCMGAEPEQIEAAYPNLLSITGPQQYESVLDAVHRALP | ||||||
Domain | 134-371 | Radical SAM core | ||||
Sequence: LTPRHYAYLKISEGCNNRCSFCIIPKLRGDLVSRPAGEVLREAEKLVKAGVKELLVVSQDTSAYGVDLKYAESPWQDRNVRARFYDLAKELGELGAWVRLQYVYPYPHVDEVIGLMAQGKVLPYLDIPFQHASPEVLKQMKRPAAQDKTLARIKQWREICPELTLRSTFIVGFPGETDSDFAYLLDWLEQAEIDRVGAFKYEAVRGATSNALPDQVSDEVKTERWNALMARQQKISAR | ||||||
Domain | 374-440 | TRAM | ||||
Sequence: KRKVGTRQQVIIDEVGPTVSKGRSKADAPQIDGSVYLTSRRPLRVGEIVTAKIERADAYDLHGSVAG |
Sequence similarities
Belongs to the methylthiotransferase family. RimO subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length441
- Mass (Da)48,776
- Last updated2006-04-18 v1
- Checksum4A10D6DB6D54CDDA
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000301 EMBL· GenBank· DDBJ | ABD88170.1 EMBL· GenBank· DDBJ | Genomic DNA |