Q21227 · APR1_CAEEL

  • Protein
    Adenomatous polyposis coli protein-related protein 1
  • Gene
    apr-1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Has a role in endoderm cell specification and pharyngeal development (PubMed:9288750).
Required for the migration of epithelial cells, organization of the anterior seam cells and ceh-13 expression during embryo morphogenesis. Prevents hyperactivation of the Wnt signaling pathway during endoderm development, probably by preventing hmp-2 nuclear translocation (PubMed:20805471).
During larval development, apr-1 is required for expression of lin-39 in P3-8.p (PubMed:10766743).
Shown to negatively regulate Wnt signaling in vulval precursor cells (PubMed:12023306).
Has a role in cell division by establishing the polarity of the mother cell which forms the asymmetries of the daughter nuclei (PubMed:17276345).
During the L4 larval stage, it is required for the asymmetric division and self-renewal of seam cells (PubMed:19607822).
Thought to regulate export of wrm-1 from the nucleus possibly as part of a complex involving pry-1 (PubMed:12023307).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentadherens junction
Cellular Componentbeta-catenin destruction complex
Cellular Componentcatenin complex
Cellular Componentcell cortex
Cellular Componentcytoplasm
Cellular Componentnucleus
Molecular Functionbeta-catenin binding
Molecular Functiongamma-catenin binding
Molecular Functionmicrotubule binding
Biological Processasymmetric cell division
Biological Processcanonical Wnt signaling pathway
Biological Processcell fate specification
Biological Processcell migration
Biological Processembryo development ending in birth or egg hatching
Biological Processembryonic digestive tract morphogenesis
Biological Processembryonic morphogenesis
Biological Processendoderm development
Biological Processendodermal cell fate specification
Biological Processengulfment of apoptotic cell
Biological Processnegative regulation of canonical Wnt signaling pathway
Biological Processnegative regulation of cell division
Biological Processnegative regulation of microtubule depolymerization
Biological Processnegative regulation of vulval development
Biological Processnegative regulation of Wnt signaling pathway
Biological Processnematode larval development
Biological Processnervous system development
Biological Processpattern specification process
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processpositive regulation of Wnt signaling pathway
Biological Processprotein transport
Biological Processregulation of cell migration
Biological Processregulation of embryonic cell shape
Biological Processreproductive process
Biological Processvulval development

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Adenomatous polyposis coli protein-related protein 1
  • Short names
    APC-related protein 1

Gene names

    • Name
      apr-1
    • ORF names
      K04G2.8

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q21227
  • Secondary accessions
    • O62302

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Nucleus
Note: Found in clusters near the ends of microtubules that extend into regions of actively migrating plasma membranes. Shuttles between the cytoplasm and nucleus.

Keywords

Phenotypes & Variants

Disruption phenotype

Worms exhibit lack of endoderm, excessive pharyngeal tissue and premature division of the E daughter blastomeres during embryogenesis. Two-thirds arrest during embryogenesis and the remaining third during the L1 stage (PubMed:9288750).
RNAi-mediated knockdown causes partial nuclear re-localization of hmp-2 in the embryonic epidermis and the production of supernumerary gut nuclei probably resulting from epithelial cell hyperproliferation (PubMed:20805471).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis338In bp298; at the L4 larval stage, seam cells divide asymmetrically as opposed to symmetrically leading to an increased number of seam cells at the young adult stage. Defective asymmetric division of the V5.p seam cell and 45.5% of animals develop two postdeirids due to symmetric division of the V5.p seam cell.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003472501-1188

Proteomic databases

Expression

Tissue specificity

During the L1 stage, expressed in vulval precursor cells (P3-8.p), seam cells and excretory cells.

Gene expression databases

Interaction

Subunit

Interacts (via N-terminus) with bar-1 and hmp-2; the interaction with hmp-2 is relatively weak. Interacts (via C-terminus) with pry-1 (via N-terminus). Probably associates with bar-1, gsk-3, pry-1 in a complex.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for compositional bias, region, repeat.

TypeIDPosition(s)Description
Compositional bias1-35Polar residues
Region1-50Disordered
Region1-486Required for interaction with bar-1 and hmp-2
Compositional bias36-50Basic and acidic residues
Repeat314-358ARM
Region579-624Disordered
Region600-1188Required for interaction with pry-1
Compositional bias604-624Polar residues
Region670-702Disordered
Region726-751Disordered
Compositional bias778-827Polar residues
Region778-952Disordered
Compositional bias870-901Basic and acidic residues
Compositional bias906-948Polar residues
Compositional bias1003-1059Polar residues
Region1003-1092Disordered
Region1157-1181Disordered
Compositional bias1159-1181Polar residues

Sequence similarities

Belongs to the adenomatous polyposis coli (APC) family.

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q21227-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    1,188
  • Mass (Da)
    131,990
  • Last updated
    2008-09-02 v2
  • Checksum
    F3CDA53B5D3527A0
MSSSSSDENETTIHRTGSNTGGSGIYSQPRAGSSKRTSNVRHDVSDVDDEEEHYARFREDTAIEVDDAITVLLSSLHFEHKRDIVPTDEDDNKLRELHEKIFALITSESDVNRKRRLKKALPASNCVREQVYYLRRKPSTPPASYYHRLNAALHTIVKESFGEEYRKVATVLGLVEALAEVLILEVHTFGINETNPGEHRNIRKLIANALTNLTYGQIHSKRRLCSYDGFIRCVVRIVIESPNITQVYAGLIRNLSWNADSGMSEALQPTVHALSIAAVHAHTHRFDVTATLSALWNLAGHSVENKRTICDTPNCLKVLASLLSPDARFTSLVDSATGILKYVSQYLANTSTHLELRSLLITRMLTLLKSASFTCVTNTLGAIANLIVKDPHMQQMIRQDMAAVQQLNVLRNSNRDDIRTAVKSVLNTLNQPCSHRYGDMSHSVGGGATGMQMLSEPQLQMQTSHHAYHGTASPRLLSLRATRASPGKYIQPQAQQQLIQTPQVDQRSSSLPRHFAVQRNGFVMAQSYNQQMDQHQQQQMIYQLQQQQQIMFQTEDQAQMEHHQQIMYLQQQQQQFHQIQQQQQMQKAQEADPVPPTDDDLDIPTSTVMGTRSNSERSLGSMNPGSVMTNWNSSLDTAANSSRALSPVSYNDIPASPTMCAQVFNLPKSTESEHHQLTSQQQNTTHYSSGSANTMTRSDGATTVPMDNIITPTYAILNPILVHEQTPNGTVPRKTSEELDSPDDVLPGPSLEEEEGDYAIIGGAAQKTDDELLTRSIQSEMPTSSSTPKMKVSPRLNGFFSPTQKTTSSPAWSHPDTSPIPKSSSHRTQPNRRQDASDADRLLMESIMSEMPKSRIISPRLAGTQQYLEPEPERRSHSKNEEADRRDAFTASHEPSDHNGIDVARGSDWSPQQQLHRMESLESQASSEDSFGLTAEEPNSSTSGAAANTMRFDDEIDASLPMDCVDDDDYDYTYDHFEDYEDEEDPDATQFDDGVDAQLTIDCSMISSGSGSSQRNETTTTSRDSKALATSTPKGSASSLPGVRQATRVSTNGKSRLPVPKTNGSLVDKNPKPIIASRRPRLPPKPTLLKDKHYPEEDSIENQTRDDTIYVNAPVVEAEQERIYMNALKQQKNIEQSPSIGNGSPIAKSAIVTPYNYQKPPFTGRNNGEMSNEKSVTPNPKQMLVTIV

Q21227-2

  • Name
    a
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1I6CM53A0A1I6CM53_CAEELapr-1892

Features

Showing features for compositional bias, alternative sequence.

TypeIDPosition(s)Description
Compositional bias1-35Polar residues
Compositional bias36-50Basic and acidic residues
Alternative sequenceVSP_052859552-553in isoform a
Compositional bias604-624Polar residues
Compositional bias778-827Polar residues
Compositional bias870-901Basic and acidic residues
Compositional bias906-948Polar residues
Compositional bias1003-1059Polar residues
Compositional bias1159-1181Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF013950
EMBL· GenBank· DDBJ
AAC47747.1
EMBL· GenBank· DDBJ
mRNA
BX284601
EMBL· GenBank· DDBJ
CAB00045.1
EMBL· GenBank· DDBJ
Genomic DNA
BX284601
EMBL· GenBank· DDBJ
CAB00048.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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