Q20117 · GSH1_CAEEL
- ProteinGlutamate--cysteine ligase
- Genegcs-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids654 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays a role in resistance to arsenite (PubMed:23593298).
May play a role in the oxidative stress response induced by reactive oxygen species produced during infection by pathogenic bacteria (PubMed:22216003).
Together with trxr-1, required for the reduction of disulfide groups in the cuticle during molting (PubMed:21199936).
May play a role in the oxidative stress response induced by reactive oxygen species produced during infection by pathogenic bacteria (PubMed:22216003).
Together with trxr-1, required for the reduction of disulfide groups in the cuticle during molting (PubMed:21199936).
Catalytic activity
- ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H+ + phosphate
Pathway
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | glutamate-cysteine ligase complex | |
Molecular Function | ATP binding | |
Molecular Function | glutamate-cysteine ligase activity | |
Biological Process | glutathione biosynthetic process | |
Biological Process | response to arsenic-containing substance | |
Biological Process | response to heat | |
Biological Process | response to reactive oxygen species | |
Biological Process | response to superoxide |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamate--cysteine ligase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ20117
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown of gsr-1 in mutants lacking trxr-1 causes an arrest during larval molting characterized by a partial detachment of the old cuticle and an impaired ability to reduce cuticle components. Also results in growth arrest during the postdauer molt (PubMed:21199936).
RNAi-mediated knockdown causes a severe loss of viability upon arsenite treatment (PubMed:23593298).
RNAi-mediated knockdown causes a severe loss of viability upon arsenite treatment (PubMed:23593298).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000192566 | 1-654 | Glutamate--cysteine ligase | |||
Sequence: MGLLTKGSPLTWAETVPHIDYIKKHGIAQFINLYHRLKSRHGDQLKWGDEIEYTIVKFDDANKKVRVSCKAEELLNKLQAEEQVNAMLGTANRFLWRPEFGSYMIEGTPGMPYGGLIACFNIVEANMKLRRQVVKKLLKKDETCLSISFPSLGVPGFTFPEVAADRKNDDAANSVFWPEQAVFLGHPRFKNLTKNIKGRRGSKVAINVPIFKDTNTPSPFVEDLSALGGPDDTRDAKPDHIYMDHMGFGMGCCCLQVTFQAVNVDEARWLYDQLTPITPILLALSAATPIFRGKLSNVDSRWDIISASVDDRTPEERGLEPLKNSKWVIDKSRYDSTDCYIYPCSVGYNDIPLQYDETIYKQLIDGNIDEPLAKHIAHMFIRDPHQVFRERIEQDDEKSSEHFETIQSSNWMNMRFKPPPPDAPEIGWRVEFRPTEVQLTDFENAAYCCFVVLLTRMMISFRLTYLMPISMVTENMKRAQQKDAVLNQKFLFRKGLAECKSAPENLKGSEKCGPPSQDIEEMSIDEIINGKKNGFPGLISLIRQFLDSADVDVDTRCTISQYLNFISKRATGEINTLAHWTRGFVQSHPAYKHDSDVNDNIVYDLLKKMDAISNGEDHCEKLLGCYRSKTDHAISAAVRKAEEHMIVSSQKRAH |
Proteomic databases
Expression
Tissue specificity
Expressed in intestine upon arsenite treatment.
Induction
By oxidative stress such as arsenite treatment.
Gene expression databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q20117 | E01A2.1 Q966L2 | 2 | EBI-311822, EBI-311817 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length654
- Mass (Da)74,369
- Last updated2003-04-11 v2
- Checksum37EA2F5DFC2BEAB5
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z54218 EMBL· GenBank· DDBJ | CAA90955.2 EMBL· GenBank· DDBJ | Genomic DNA |