Q1YEB0 · Q1YEB0_AURMS

Function

function

Catalyzes the ATP-dependent 2-thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s2C32). The sulfur atoms are provided by the cysteine/cysteine desulfurase (IscS) system.

Miscellaneous

The thiolation reaction likely consists of two steps: a first activation step by ATP to form an adenylated intermediate of the target base of tRNA, and a second nucleophilic substitution step of the sulfur (S) atom supplied by the hydrosulfide attached to the Fe-S cluster.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit. The cluster is chelated by three Cys residues, the fourth Fe has a free coordination site that may bind a sulfur atom transferred from the persulfide of IscS.

Pathway

tRNA modification.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site155[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site158[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site246[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionsulfurtransferase activity
Molecular FunctiontRNA binding
Biological ProcesstRNA thio-modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-cytidine(32) 2-sulfurtransferase
  • EC number
  • Alternative names
    • Two-thiocytidine biosynthesis protein A
    • tRNA 2-thiocytidine biosynthesis protein TtcA

Gene names

    • Name
      ttcA
    • ORF names
      SI859A1_01165

Organism names

Accessions

  • Primary accession
    Q1YEB0

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for region, domain, motif.

TypeIDPosition(s)Description
Region16-38Disordered
Domain75-239tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal
Motif80-85PP-loop motif

Sequence similarities

Belongs to the TtcA family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    305
  • Mass (Da)
    33,754
  • Last updated
    2006-05-02 v1
  • Checksum
    82B2E773A779CECC
MTTEAGMLDLETARAADAQDDDAATPAAEDGAGSAHPLFRDCPSSVTFKKLRKRLLREMRETIEAHAMARPGARWLVGLSGGKDSYALLALLSDLKYRGLLPVDILACNLDQGQPNFPKHVLPDFLSRYDIPHRIEYRDTYSVVTEKVPSNATYCALCSRLRRGNLYRIAREEGCEALVLGHHADDCLETFFMNLFHGGRLSAMPAKLINDEGDLMVLRPLIRSVEEDIAKFSAAMKFPIIPCDLCGSQDGLQRNVTKQMIADLERRFPGRKQVMLRALANVRPGHLLDPSLFDFAGLAPASPTT

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAPJ01000008
EMBL· GenBank· DDBJ
EAS48681.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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