Q1YDX4 · Q1YDX4_AURMS
- ProteintRNA N6-adenosine threonylcarbamoyltransferase
- GenetsaD
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids367 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.
Catalytic activity
- adenosine37 in tRNA + L-threonylcarbamoyladenylate = AMP + H+ + N6-L-threonylcarbamoyladenosine37 in tRNA
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 123 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 127 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 145-149 | substrate | ||||
Sequence: LVSGG | ||||||
Binding site | 178 | substrate | ||||
Sequence: D | ||||||
Binding site | 191 | substrate | ||||
Sequence: G | ||||||
Binding site | 195 | substrate | ||||
Sequence: E | ||||||
Binding site | 291 | substrate | ||||
Sequence: N | ||||||
Binding site | 319 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | iron ion binding | |
Molecular Function | N(6)-L-threonylcarbamoyladenine synthase activity | |
Biological Process | tRNA threonylcarbamoyladenosine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA N6-adenosine threonylcarbamoyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Aurantimonadaceae > Aurantimonas
Accessions
- Primary accessionQ1YDX4
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 36-326 | Gcp-like | ||||
Sequence: IHSNVVLSQVEEHAAFGGVVPEIAARAHVEALDGIVKAAMDEAGIGFDELSAVAATTGPGLVGGLIVGAMTGKAIAAARRLPFLAINHLEGHALTPRLTDRLAYPYLLLLVSGGHSQIVLVRGLGAYERWGTTIDDALGEAFDKTAKLLGLPNPGGPHVERRAAAGDPKRFALPRPMKGNPRLDFSFSGLKTALRMAAQAAAPLSPTDVDDLCAGFQAAAADSLADRVGRALTQFRDRFPDLADPVLAVAGGVAANRAIRTALEAQTARTGVRLVAPPLALCTDNAAMIAY |
Sequence similarities
Belongs to the KAE1 / TsaD family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length367
- Mass (Da)37,990
- Last updated2006-05-02 v1
- Checksum110BFAFE5862144F
Keywords
- Technical term