Q1RFI7 · FRMA_ECOUT
- ProteinS-(hydroxymethyl)glutathione dehydrogenase
- GenefrmA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids369 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Has high formaldehyde dehydrogenase activity in the presence of glutathione and catalyzes the oxidation of normal alcohols in a reaction that is not GSH-dependent. In addition, hemithiolacetals other than those formed from GSH, including omega-thiol fatty acids, also are substrates. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADH-dependent reduction of S-nitrosoglutathione.
Catalytic activity
- NADP+ + S-(hydroxymethyl)glutathione = H+ + NADPH + S-formylglutathione
- H+ + NADH + S-nitrosoglutathione = NAD+ + S-(hydroxysulfenamide)glutathioneThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 2 Zn2+ ions per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 40 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: C | ||||||
Binding site | 62 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: H | ||||||
Binding site | 92 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 95 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 98 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 106 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 169 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | alcohol dehydrogenase (NAD+) activity | |
Molecular Function | S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity | |
Molecular Function | S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity | |
Molecular Function | zinc ion binding | |
Biological Process | formaldehyde catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameS-(hydroxymethyl)glutathione dehydrogenase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionQ1RFI7
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000341285 | 1-369 | S-(hydroxymethyl)glutathione dehydrogenase | |||
Sequence: MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDAFTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGKTNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEANHEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY |
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length369
- Mass (Da)39,347
- Last updated2006-05-16 v1
- Checksum760D615FF801ED20
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000243 EMBL· GenBank· DDBJ | ABE05877.1 EMBL· GenBank· DDBJ | Genomic DNA |