Q1R8U3 · PTYBC_ECOUT
- ProteinPTS system N-acetylmuramic acid-specific EIIBC component
- GenemurP
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids474 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N-acetylmuramic acid (MurNAc) transport, yielding cytoplasmic MurNAc-6-P. Is also able to take up anhydro-N-acetylmuramic acid (anhMurNAc), but cannot phosphorylate the carbon 6, probably because of the 1,6-anhydro ring.
Catalytic activity
- N-acetyl-beta-D-muramate(out) + N(pros)-phospho-L-histidyl-[protein] = N-acetyl-beta-D-muramate 6-phosphate(in) + L-histidyl-[protein]
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 29 | Phosphocysteine intermediate; for EIIB activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | kinase activity | |
Molecular Function | protein-N(PI)-phosphohistidine-sugar phosphotransferase activity | |
Molecular Function | protein-phosphocysteine-N-acetylmuramate phosphotransferase system transporter activity | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system | |
Biological Process | phosphorylation |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended namePTS system N-acetylmuramic acid-specific EIIBC component
- Alternative names
Including 2 domains:
- Recommended nameN-acetylmuramic acid-specific phosphotransferase enzyme IIB component
- EC number
- Alternative names
- Recommended nameN-acetylmuramic acid permease IIC component
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionQ1R8U3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-123 | Cytoplasmic | |||
Transmembrane | 124-144 | Helical | |||
Topological domain | 145-157 | Periplasmic | |||
Transmembrane | 158-178 | Helical | |||
Topological domain | 179-180 | Cytoplasmic | |||
Transmembrane | 181-201 | Helical | |||
Topological domain | 202-217 | Periplasmic | |||
Transmembrane | 218-238 | Helical | |||
Topological domain | 239-260 | Cytoplasmic | |||
Transmembrane | 261-281 | Helical | |||
Topological domain | 282-301 | Periplasmic | |||
Transmembrane | 302-322 | Helical | |||
Topological domain | 323-334 | Cytoplasmic | |||
Transmembrane | 335-355 | Helical | |||
Topological domain | 356-368 | Periplasmic | |||
Transmembrane | 369-389 | Helical | |||
Topological domain | 390-393 | Cytoplasmic | |||
Transmembrane | 394-414 | Helical | |||
Topological domain | 415-440 | Periplasmic | |||
Transmembrane | 441-461 | Helical | |||
Topological domain | 462-474 | Cytoplasmic | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000248955 | 1-474 | PTS system N-acetylmuramic acid-specific EIIBC component | ||
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-89 | PTS EIIB type-1 | |||
Domain | 115-474 | PTS EIIC type-1 | |||
Domain
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length474
- Mass (Da)49,815
- Last updated2006-05-16 v1
- Checksum76E0204B5F0B8AC7
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000243 EMBL· GenBank· DDBJ | ABE08221.1 EMBL· GenBank· DDBJ | Genomic DNA |