Q1R8K0 · TRMJ_ECOUT
- ProteintRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ
- GenetrmJ
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids246 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA.
Catalytic activity
- cytidine32 in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine32 in tRNA + H+ + S-adenosyl-L-homocysteine
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 79-81 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: TSA | ||||||
Binding site | 114 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 134 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 141-143 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: SSL |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | RNA binding | |
Molecular Function | tRNA (uracil-2'-O-)-methyltransferase activity | |
Biological Process | tRNA nucleoside ribose methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionQ1R8K0
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000313854 | 1-246 | tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ | |||
Sequence: MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWLATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQNKGNKAE |
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length246
- Mass (Da)27,048
- Last updated2006-05-16 v1
- Checksum83C1AE4507E097D2
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000243 EMBL· GenBank· DDBJ | ABE08314.1 EMBL· GenBank· DDBJ | Genomic DNA |