Q1R6B5 · NANA_ECOUT
- ProteinN-acetylneuraminate lyase
- GenenanA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids297 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate.
Catalytic activity
- aceneuramate = aldehydo-N-acetyl-D-mannosamine + pyruvate
Pathway
Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 1/5.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 47 | aceneuramate (UniProtKB | ChEBI) | |||
Binding site | 48 | aceneuramate (UniProtKB | ChEBI) | |||
Active site | 137 | Proton donor | |||
Active site | 165 | Schiff-base intermediate with substrate | |||
Binding site | 167 | aceneuramate (UniProtKB | ChEBI) | |||
Binding site | 189 | aceneuramate (UniProtKB | ChEBI) | |||
Binding site | 191 | aceneuramate (UniProtKB | ChEBI) | |||
Binding site | 192 | aceneuramate (UniProtKB | ChEBI) | |||
Binding site | 208 | aceneuramate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | N-acetylneuraminate lyase activity | |
Biological Process | carbohydrate metabolic process | |
Biological Process | N-acetylneuraminate catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acetylneuraminate lyase
- EC number
- Short namesNAL ; Neu5Ac lyase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionQ1R6B5
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000066927 | 1-297 | N-acetylneuraminate lyase | ||
Interaction
Subunit
Homotetramer.
Structure
Sequence
- Sequence statusComplete
- Length297
- Mass (Da)32,593
- Last updated2006-05-16 v1
- ChecksumBA9F30B4A7624167
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000243 EMBL· GenBank· DDBJ | ABE09099.1 EMBL· GenBank· DDBJ | Genomic DNA |