Q1PWK5 · Q1PWK5_KUEST

  • Protein
    tRNA-specific 2-thiouridylase MnmA
  • Gene
    mnmA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Features

Showing features for binding site, active site, site.

133650100150200250300
Type
IDPosition(s)Description
Binding site13ATP (UniProtKB | ChEBI)
Active site81Nucleophile
Binding site105ATP (UniProtKB | ChEBI)
Site106Interaction with tRNA
Active site177Cysteine persulfide intermediate
Site316Interaction with tRNA

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uridine 2-sulfurtransferase activity
Biological ProcesstRNA wobble position uridine thiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-specific 2-thiouridylase MnmA
  • EC number

Gene names

    • Name
      mnmA
    • ORF names
      kustc0863

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Bacteria > Planctomycetota > Candidatus Brocadiia > Candidatus Brocadiales > Candidatus Brocadiaceae > Candidatus Kuenenia

Accessions

  • Primary accession
    Q1PWK5

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region127-129Interaction with tRNA
Domain196-249tRNA-specific 2-thiouridylase MnmA-like central
Domain257-332tRNA-specific 2-thiouridylase MnmA-like C-terminal
Region283-284Interaction with tRNA

Sequence similarities

Belongs to the MnmA/TRMU family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    336
  • Mass (Da)
    37,984
  • Last updated
    2006-05-16 v1
  • Checksum
    AF13661DDEE896DF
MVEQGYEVVGLFMRLGLEKLDAMTKTKVCCSLEDANDARTVAEQLGIQFHVLNFKEAFDHIIDHFCTEYLNGRTPNPCIMCNQELKFGKLLNFAKMLNADFVATGHYAKVEKHNDRFLLKKGDDLNKEQSYVLFSLNQEQLSKTLFPLGTLTKESVRRIAKDLNLKTKDKPESQDICFVLDNNYHSLLYERYGNHIIPGPIKDRQGNILGEHQGAPFFTIGQRKGLGVAFGSPRYVVDINPRENAVIIGTDKDLEETGLIASSLNWISVQQLELPMEVQAKIRYHHMPAHATVSPFGTDKVRVHFNEPQKAITPGQAVVFYDNDTVVGGGWIDRKD

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CT573073
EMBL· GenBank· DDBJ
CAJ71608.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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