Q1PDV6 · PBL27_ARATH

Function

function

Receptor-like cytoplasmic kinase involved in the transduction of signal between the host cell surface chitin receptor complex CERK1-LYK5 and the intracellular MAPKKK5-dependent mitogen-activated protein kinase (MAPK) cascade that leads to chitin-induced immunity (PubMed:24750441, PubMed:27679653).
Phosphorylates and activates MAPKKK5 when phosphorylated by CERK1 after elicitation by chitin (PubMed:27679653).

Catalytic activity

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site89-97ATP (UniProtKB | ChEBI)
Binding site112ATP (UniProtKB | ChEBI)
Active site210Proton acceptor

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functionprotein kinase activity
Molecular Functionprotein serine kinase activity
Molecular Functionprotein serine/threonine kinase activity
Biological Processcellular response to chitin
Biological Processdefense response to fungus
Biological Processinnate immune response
Biological Processpositive regulation of defense response to bacterium
Biological Processpositive regulation of MAPK cascade
Biological Processregulation of defense response to fungus
Biological Processregulation of innate immune response

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Serine/threonine-protein kinase PBL27
  • EC number
  • Alternative names
    • PBS1-like protein 27
    • Receptor-like cytoplasmic kinase PBL27

Gene names

    • Name
      PBL27
    • ORF names
      T28N17.90
    • Ordered locus names
      At5g18610

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q1PDV6
  • Secondary accessions
    • A0MFG6

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Cell membrane
; Lipid-anchor

Keywords

Phenotypes & Variants

Disruption phenotype

Impaired chitin-induced defense responses such as MAPK activation (e.g. MPK3/6) and callose deposition leading to reduced disease resistance against fungal (e.g. A.brassicicola) and bacterial (e.g. P.syringae pv. tomato DC3000 hrcC) infections.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis112No autophosphorylation activity. Directly phosphorylated by CERK1 in vitro. Impaired CERK1-mediated phosphorylation; when associated with A-244; A-245 and A-250.
Mutagenesis244Impaired CERK1-mediated phosphorylation; when associated with E-112; A-245 and A-250.
Mutagenesis245Impaired CERK1-mediated phosphorylation; when associated with E-112; A-244 and A-250.
Mutagenesis250Impaired CERK1-mediated phosphorylation; when associated with E-112; A-244 and A-245.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 11 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, lipidation, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004386181-513Serine/threonine-protein kinase PBL27
Lipidation4S-palmitoyl cysteine
Lipidation7S-palmitoyl cysteine
Modified residue244Phosphoserine; by CERK1
Modified residue245Phosphothreonine; by CERK1
Modified residue250Phosphothreonine; by CERK1
Modified residue392Phosphoserine
Modified residue401Phosphoserine

Post-translational modification

Phosphorylated by CERK1 upon elicitation by chitin.
Palmitoylation at Cys-4 and Cys-7 are required for plasma membrane location.

Keywords

Proteomic databases

PTM databases

Expression

Induction

Levels are regulated in a proteasome-dependent manner (at proteome level).

Gene expression databases

Interaction

Subunit

Interacts with CERK1 (preferentially unphosphorylated) at the plasma membrane (PubMed:24750441, PubMed:27679653).
Binds to MAPKKK5 at the plasma membrane; disassociation is induced by chitin perception by the CERK1 complex. Associates also with MAPKKK3 (PubMed:27679653).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-61Disordered
Compositional bias16-30Basic and acidic residues
Compositional bias39-59Basic and acidic residues
Domain83-360Protein kinase
Compositional bias365-381Polar residues
Region365-513Disordered
Compositional bias398-414Polar residues
Compositional bias455-472Polar residues
Compositional bias473-503Basic and acidic residues

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    513
  • Mass (Da)
    55,931
  • Last updated
    2011-05-03 v1
  • Checksum
    9FC534796025AB87
MSGCLPCFGSSAKDAASKDSVKKELSAKDGSVTQSHHISLDKSKSRRGPEQKKELTAPKEGPTAHIAAQTFTFRELAAATKNFRPECLLGEGGFGRVYKGRLETTGQIVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMTIAAGAAKGLEYLHDKANPPVIYRDLKSSNILLGDGYHPKLSDFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTLKSDVYSFGVVFLELITGRKAIDNARAPGEHNLVAWARPLFKDRRKFPKMADPSLQGRYPMRGLYQALAVAAMCLQEQAATRPLIGDVVTALTYLASQTFDPNAPSGQNSRSGSGPPFIRTRDDRRSLGDGSSLDSPAETRSRLGSPATHKNSPDYRRRDMVREVNAGSEGGSETGGGSGRKWGLSDLEGQESQRGSPASVGRSSRGTPRNRDLDRERAVAEAKVWGENWRERKRATNGPGSFDSTND

Sequence caution

The sequence ABK28701.1 differs from that shown. Reason: Erroneous termination Extended C-terminus.

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias16-30Basic and acidic residues
Compositional bias39-59Basic and acidic residues
Compositional bias365-381Polar residues
Compositional bias398-414Polar residues
Compositional bias455-472Polar residues
Compositional bias473-503Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC051627
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AC069328
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CP002688
EMBL· GenBank· DDBJ
AED92585.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED92586.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
ANM70412.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ446962
EMBL· GenBank· DDBJ
ABE66165.1
EMBL· GenBank· DDBJ
mRNA
DQ653292
EMBL· GenBank· DDBJ
ABK28701.1
EMBL· GenBank· DDBJ
mRNA Sequence problems.

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp