Q1NAJ2 · MAND_SPHSS

Function

function

Catalyzes the dehydration of D-mannonate. Has no detectable activity with a panel of 70 other acid sugars (in vitro).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Kinetics

kcat is 2.0 sec-1 with D-mannonate.

Pathway

Carbohydrate metabolism; pentose and glucuronate interconversion.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site38substrate
Binding site123substrate
Active site160Proton donor/acceptor
Binding site211Mg2+ (UniProtKB | ChEBI)
Active site213Proton donor/acceptor
Binding site237Mg2+ (UniProtKB | ChEBI)
Binding site263Mg2+ (UniProtKB | ChEBI)
Binding site263substrate
Binding site284substrate
Binding site313substrate
Site315Important for activity and substrate specificity; Ala is observed in family members with high D-mannonate dehydratase activity that have no activity with D-gluconate
Binding site317substrate
Binding site340substrate

GO annotations

AspectTerm
Molecular Functionmagnesium ion binding
Molecular Functionmannonate dehydratase activity
Biological Processamino acid catabolic process
Biological Processcarbohydrate catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    D-mannonate dehydratase
  • EC number
  • Short names
    ManD

Gene names

    • ORF names
      SKA58_08619

Organism names

Accessions

  • Primary accession
    Q1NAJ2

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004298741-403D-mannonate dehydratase

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    403
  • Mass (Da)
    44,964
  • Last updated
    2006-05-30 v1
  • Checksum
    FFFF0B61540FF4C6
MPKIIDAKVIITCPGRNFVTLKIMTDEGVYGLGDATLNGRELAVASYLTDHVIPCLIGRDAHRIEDLWQYLYKGAYWRRGPVTMTAIAAVDMALWDIKGKIAGLPVYQLLGGASREGVMVYGHANGTTIEDTVKVALDYQAQGYKAIRLQCGVPGMASTYGVSKDKYFYEPADADLPTENIWNTSKYLRIVPELFKAARESLGWDVHLLHDIHHRLTPIEAGRLGQDLEPYRPFWLEDATPAENQEAFRLIRQHTTAPLAVGEIFNSIWDAKDLIQNQLIDYIRATVVHAGGITHLRRIAALADLYQIRTGCHGATDLSPVCMAAALHFDLSVPNFGIQEYMRHMPETDAVFPHAYTFADGMMHPGDQPGLGVDIDEDLAAGYEYKRAFLPVNRLEDGTMFNW

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAQG01000013
EMBL· GenBank· DDBJ
EAT08014.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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