Q1NAJ2 · MAND_SPHSS
- ProteinD-mannonate dehydratase
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids403 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the dehydration of D-mannonate. Has no detectable activity with a panel of 70 other acid sugars (in vitro).
Catalytic activity
- D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Kinetics
kcat is 2.0 sec-1 with D-mannonate.
Pathway
Carbohydrate metabolism; pentose and glucuronate interconversion.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 38 | substrate | ||||
Sequence: N | ||||||
Binding site | 123 | substrate | ||||
Sequence: H | ||||||
Active site | 160 | Proton donor/acceptor | ||||
Sequence: Y | ||||||
Binding site | 211 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 213 | Proton donor/acceptor | ||||
Sequence: H | ||||||
Binding site | 237 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 263 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 263 | substrate | ||||
Sequence: E | ||||||
Binding site | 284 | substrate | ||||
Sequence: R | ||||||
Binding site | 313 | substrate | ||||
Sequence: H | ||||||
Site | 315 | Important for activity and substrate specificity; Ala is observed in family members with high D-mannonate dehydratase activity that have no activity with D-gluconate | ||||
Sequence: A | ||||||
Binding site | 317 | substrate | ||||
Sequence: D | ||||||
Binding site | 340 | substrate | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | magnesium ion binding | |
Molecular Function | mannonate dehydratase activity | |
Biological Process | amino acid catabolic process | |
Biological Process | carbohydrate catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameD-mannonate dehydratase
- EC number
- Short namesManD
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Sphingomonadaceae > Sphingomonas
Accessions
- Primary accessionQ1NAJ2
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000429874 | 1-403 | D-mannonate dehydratase | |||
Sequence: MPKIIDAKVIITCPGRNFVTLKIMTDEGVYGLGDATLNGRELAVASYLTDHVIPCLIGRDAHRIEDLWQYLYKGAYWRRGPVTMTAIAAVDMALWDIKGKIAGLPVYQLLGGASREGVMVYGHANGTTIEDTVKVALDYQAQGYKAIRLQCGVPGMASTYGVSKDKYFYEPADADLPTENIWNTSKYLRIVPELFKAARESLGWDVHLLHDIHHRLTPIEAGRLGQDLEPYRPFWLEDATPAENQEAFRLIRQHTTAPLAVGEIFNSIWDAKDLIQNQLIDYIRATVVHAGGITHLRRIAALADLYQIRTGCHGATDLSPVCMAAALHFDLSVPNFGIQEYMRHMPETDAVFPHAYTFADGMMHPGDQPGLGVDIDEDLAAGYEYKRAFLPVNRLEDGTMFNW |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length403
- Mass (Da)44,964
- Last updated2006-05-30 v1
- ChecksumFFFF0B61540FF4C6
Keywords
- Technical term