Q1KUY9 · Q1KUY9_9ROSI
- ProteinNADPH-dependent diflavin oxidoreductase 1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids630 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of the anamorsin/DRE2 homolog, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins.
Catalytic activity
- 2 oxidized [2Fe-2S]-[protein] + NADPH = 2 reduced [2Fe-2S]-[protein] + NADP+ + H+
Cofactor
Protein has several cofactor binding sites:
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 14-19 | FMN (UniProtKB | ChEBI) | |||
Binding site | 61-64 | FMN (UniProtKB | ChEBI) | |||
Binding site | 134 | FMN (UniProtKB | ChEBI) | |||
Binding site | 373 | FAD (UniProtKB | ChEBI) | |||
Binding site | 403-406 | FAD (UniProtKB | ChEBI) | |||
Binding site | 437-440 | FAD (UniProtKB | ChEBI) | |||
Binding site | 479 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 548-549 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 554-558 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 591 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 629 | FAD (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | NADP binding | |
Molecular Function | NADPH-hemoprotein reductase activity | |
Biological Process | iron-sulfur cluster assembly |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH-dependent diflavin oxidoreductase 1
- EC number
- Alternative names
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Cleomaceae > New World clade > Tarenaya
Accessions
- Primary accessionQ1KUY9
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 8-152 | Flavodoxin-like | |||
Domain | 226-471 | FAD-binding FR-type | |||
Sequence similarities
Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length630
- Mass (Da)70,731
- Last updated2006-05-30 v1
- Checksum40149C03A9C3CB3F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ415920 EMBL· GenBank· DDBJ | ABD96847.1 EMBL· GenBank· DDBJ | Genomic DNA |