Q1KLK0 · SMTB_CHLAU
- ProteinSuccinyl-CoA--L-malate CoA-transferase beta subunit
- GenesmtB
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids405 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in the 3-hydroxypropionate cycle used for autotrophic carbon dioxide fixation. Catalyzes the transfer of CoA moiety from succinyl-CoA to L-malate to yield L-malyl-CoA. It is highly specific for succinyl-CoA as the CoA donor, however it can accept L-citramalate instead of L-malate as the CoA acceptor.
Catalytic activity
- (S)-malate + succinyl-CoA = (S)-malyl-CoA + succinate
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.5 mM | succinyl-CoA | |||||
1.1 mM | L-citramalate | |||||
1.3 mM | L-malate |
pH Dependence
Optimum pH is 6.5.
Temperature Dependence
Optimum temperature is 55 degrees Celsius.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 175 | Nucleophile | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | CoA-transferase activity | |
Molecular Function | succinate-citramalate CoA-transferase activity | |
Biological Process | carbon fixation by 3-hydroxypropionate cycle |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSuccinyl-CoA--L-malate CoA-transferase beta subunit
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Chloroflexota > Chloroflexia > Chloroflexales > Chloroflexineae > Chloroflexaceae > Chloroflexus
Accessions
- Primary accessionQ1KLK0
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000429593 | 1-405 | Succinyl-CoA--L-malate CoA-transferase beta subunit | |||
Sequence: MDGTTTTLPLAGIRVIDAATVIAAPFCATLLGEFGADVLKVEHPIGGDALRRFGTPTARGDTLTWLSESRNKRSVTLNLQHPEGARVFKELIAHSDVLCENFRPGTLEKWGLGWDVLSKINPRLIMLRVTGYGQTGPYRDRPGFARIAHAVGGIAYLAGMPKGTPVTPGSTTLADYMTGLYGCIGVLLALRHREQTGRGQYIDAALYESVFRCSDELVPAYGMYRKVRERHGSHYNEFACPHGHFQTKDGKWVAISCATDKLFARLANAMGRPELASSSVYGDQKVRLAHASDVNEIVRDWCSSLTRAEVLERCYATATPAAPLNDIADFFGDRHVHARRNLVAIDAEDLGETLIMPNVVPKLSETPGSIRSLGPKLGEHTEEVLKEILGMCDEQINDLRSKRVI |
Expression
Induction
Under autotrophic growth conditions.
Interaction
Subunit
Forms a large complex composed of six heterodimers (alpha, beta).
Structure
Sequence
- Sequence statusComplete
- Length405
- Mass (Da)44,384
- Last updated2006-05-30 v1
- Checksum58041E71692E2F4E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ472737 EMBL· GenBank· DDBJ | ABF14400.1 EMBL· GenBank· DDBJ | Genomic DNA |