Q1JUZ1 · NVD_DROME
- ProteinCholesterol 7-desaturase nvd
- Genenvd
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids429 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the production of 7-dehydrocholesterol (7-DHC or cholesta-5,7-dien-3beta-ol) by inserting a double bond (desaturating) at the C7-C8 single bond of cholesterol (PubMed:21632547).
Essential regulator of steroid biosynthesis, as this reaction is the first step in the synthesis of the steroid hormone Delta7-dafachronic acid (PubMed:16763204, PubMed:21632547).
Required for insect molting, metamorphosis and body growth throughout development via the regulation of ecdysteroid biosynthesis in the prothoracic gland (PubMed:16763204).
Essential regulator of steroid biosynthesis, as this reaction is the first step in the synthesis of the steroid hormone Delta7-dafachronic acid (PubMed:16763204, PubMed:21632547).
Required for insect molting, metamorphosis and body growth throughout development via the regulation of ecdysteroid biosynthesis in the prothoracic gland (PubMed:16763204).
Miscellaneous
Nvd_Dm plays a pivotal role in larval development in Drosophila. On this basis of the prolonged first instar larval phenotype, the gene was named 'neverland', which is the fictional island featured in J. M. Barrie's play Peter Pan, where children cease to age.
Catalytic activity
- cholesterol + H+ + NADPH + O2 = 7-dehydrocholesterol + 2 H2O + NADP+This reaction proceeds in the forward direction.
Cofactor
Note: Binds 1 [2Fe-2S] cluster per subunit.
Pathway
Steroid hormone biosynthesis; dafachronic acid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | membrane | |
Molecular Function | 2 iron, 2 sulfur cluster binding | |
Molecular Function | cholesterol 7-desaturase (NAD(P)H) activity | |
Molecular Function | metal ion binding | |
Molecular Function | oxidoreductase activity | |
Biological Process | cholesterol metabolic process | |
Biological Process | ecdysteroid biosynthetic process | |
Biological Process | instar larval development | |
Biological Process | metamorphosis | |
Biological Process | multicellular organism growth |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameCholesterol 7-desaturase nvd
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionQ1JUZ1
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 23-43 | Helical | ||||
Sequence: FVICLWTLAVTFIRIYWIFFV |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
RNAi animals show apparent growth arrest in body size compared with control animal, also loss of nvd-Dm function prevents larval growth prior to the initiation of the molting process.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000452609 | 1-429 | Cholesterol 7-desaturase nvd | |||
Sequence: MTSYSLFWMSLLKNNWKPISNDFVICLWTLAVTFIRIYWIFFVPLEWKKDLDNEKWSFLRKTENVVCYNHKRDTINRLRKLKIQKIIELPPPYPNGWYGILKSSQLKAGEATCVSCLGEDLVIFRSKKDIVFILDAYCPHLGANLGIGGSVADDCVICPFHQWKFRGTDGLCINIPYSTSVPKGSKLKKWISQEVDGFIFIWYHAEQTELPWDLPVPMGEIDDTFVYHGHNEFYINCHIQEIPENGADIAHFNAIHKKNFINGSWAQKKRLFGLGSHHWKARWSPFTGKLKYLAEVNLSHTFKLFGKFGCFRMEVSGKQIGPSIVCLEVNSYTFGKIKVFQYITPIEPMLQKVVHEFYGPRWIAPLMKIFIYGESLMFERDIKIWNHKVFNRNPILAKEDASIKKFRLWFSQFYSSNSKIYSEATNIGW |
Proteomic databases
Expression
Tissue specificity
Expressed predominantly in the prothoracic gland and weakly in brain and malpighian tubules.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 98-201 | Rieske | ||||
Sequence: YGILKSSQLKAGEATCVSCLGEDLVIFRSKKDIVFILDAYCPHLGANLGIGGSVADDCVICPFHQWKFRGTDGLCINIPYSTSVPKGSKLKKWISQEVDGFIFI |
Sequence similarities
Belongs to the cholesterol 7-desaturase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length429
- Mass (Da)50,106
- Last updated2006-06-13 v1
- ChecksumCA4D2F0D1F8191CC
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 17 | in Ref. 4; AAX33409 | ||||
Sequence: K → R | ||||||
Sequence conflict | 159 | in Ref. 4; AAX33409 | ||||
Sequence: P → T |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB232987 EMBL· GenBank· DDBJ | BAE94193.1 EMBL· GenBank· DDBJ | mRNA | ||
AE014296 EMBL· GenBank· DDBJ | ABW08586.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT021261 EMBL· GenBank· DDBJ | AAX33409.1 EMBL· GenBank· DDBJ | mRNA |