Q1HAQ0 · PCAT1_RAT
- ProteinLysophosphatidylcholine acyltransferase 1
- GeneLpcat1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids534 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Exhibits acyltransferase activity (PubMed:16864775).
Exhibits acetyltransferase activity (By similarity).
Activity is calcium-independent (By similarity).
Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (PubMed:16864775).
Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:16864775).
Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively (By similarity).
Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF) (By similarity).
May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology (PubMed:16864775).
Involved in the regulation of lipid droplet number and size (By similarity).
Exhibits acetyltransferase activity (By similarity).
Activity is calcium-independent (By similarity).
Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (PubMed:16864775).
Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:16864775).
Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively (By similarity).
Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF) (By similarity).
May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology (PubMed:16864775).
Involved in the regulation of lipid droplet number and size (By similarity).
Catalytic activity
- a 1-acyl-sn-glycero-3-phosphocholine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphocholine + CoA
- 1-acyl-sn-glycero-3-phospho-(1'-sn-glycerol) + an acyl-CoA = 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + CoAThis reaction proceeds in the forward direction.
- a 1-acyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1-acyl-2-hexadecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- a 1-acyl-sn-glycero-3-phosphate + hexadecanoyl-CoA = 1-acyl-2-hexadecanoyl-sn-glycero-3-phosphate + CoAThis reaction proceeds in the forward direction.
- 1-acyl-sn-glycero-3-phospho-(1'-sn-glycerol) + hexadecanoyl-CoA = 1-acyl-2-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + CoAThis reaction proceeds in the forward direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-O-hexadecyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1-O-hexadecyl-2-hexadecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1-O-(1Z)-alkenyl-2-hexadecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + hexadecanoyl-CoA = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + CoAThis reaction proceeds in the forward direction.
- 1-dodecanoyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1-dodecanoyl-2-hexadecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-tetradecanoyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1-tetradecanoyl-2-hexadecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-O-octadecyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1-O-octadecyl-2-hexadecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-octadecanoyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1-octadecanoyl-2-hexadecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1-(9Z-octadecenoyl)-2-hexadecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-eicosanoyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1-eicosanoyl-2-hexadecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexanoyl-CoA = 1-hexadecanoyl-2-hexanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + octanoyl-CoA = 1-hexadecanoyl-2-octanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + decanoyl-CoA = 1-hexadecanoyl-2-decanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + dodecanoyl-CoA = 1-hexadecanoyl-2-dodecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + tetradecanoyl-CoA = 1-hexadecanoyl-2-tetradecanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- (9Z)-octadecenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- (9Z,12Z)-octadecadienoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + acetyl-CoA = 1-hexadecanoyl-2-acetyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + eicosanoyl-CoA = 1-hexadecanoyl-2-eicosanoyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
- 1-O-hexadecyl-sn-glycero-3-phosphocholine + acetyl-CoA = 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CoAThis reaction proceeds in the forward direction.
Activity regulation
Activity is stimulated by Mg2+ or Mn2+.
Pathway
Lipid metabolism; phospholipid metabolism.
Features
Showing features for binding site.
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameLysophosphatidylcholine acyltransferase 1
- EC number
- Short namesLPC acyltransferase 1; LPCAT-1; LysoPC acyltransferase 1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ1HAQ0
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass type II membrane protein
Golgi apparatus membrane ; Single-pass type II membrane protein
Cell membrane ; Single-pass type II membrane protein
Note: May adopt a monotopic topology when embedded in the lipid monolayer of the lipid droplet, with both termini exposed to the cytoplasm.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-57 | Cytoplasmic | ||||
Sequence: MRLRGRGPRAAPSSSSGAGDARRLAPPGRNPFVHELRLSALQKAQVAFMTLTLFPIR | ||||||
Transmembrane | 58-78 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: LLFAAFMMLLAWPFALVASLG | ||||||
Topological domain | 79-534 | Lumenal | ||||
Sequence: PPDKEPEQPLALWRKVVDFLLKAIMRTMWFAGGFHRVAVKGRQALPTEAAILTLAPHSSYFDAIPVTMTMSSIVMKAESRDIPIWGTLIRYIRPVFVSRSDQDSRRKTVEEIKRRAQSNGKWPQIMIFPEGTCTNRTCLITFKPGAFIPGVPVQPVVLRYPNKLDTITWTWQGPGALKILWLTLCQFQNQVEIEFLPVYCPSEEEKRNPALYASNVRRVMAKALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGLKPENLEKDLDKYSESARMKRGEKIRLPEFAAYLEVPVSDALEDMFSLFDESGGGEIDLREYVVALSVVCRPSQTLATIQLAFKMYGSPEDGSIDEADLSCILKTALGISELTVTDLFQAIDQEERGRITFDDFCGFAEMYPDFAEDYLYPDQTHSDSCAQTPPAPTPNGFCIDFSPEHSDFGRKNSCKKVD |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000247066 | 1-534 | Lysophosphatidylcholine acyltransferase 1 | |||
Sequence: MRLRGRGPRAAPSSSSGAGDARRLAPPGRNPFVHELRLSALQKAQVAFMTLTLFPIRLLFAAFMMLLAWPFALVASLGPPDKEPEQPLALWRKVVDFLLKAIMRTMWFAGGFHRVAVKGRQALPTEAAILTLAPHSSYFDAIPVTMTMSSIVMKAESRDIPIWGTLIRYIRPVFVSRSDQDSRRKTVEEIKRRAQSNGKWPQIMIFPEGTCTNRTCLITFKPGAFIPGVPVQPVVLRYPNKLDTITWTWQGPGALKILWLTLCQFQNQVEIEFLPVYCPSEEEKRNPALYASNVRRVMAKALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGLKPENLEKDLDKYSESARMKRGEKIRLPEFAAYLEVPVSDALEDMFSLFDESGGGEIDLREYVVALSVVCRPSQTLATIQLAFKMYGSPEDGSIDEADLSCILKTALGISELTVTDLFQAIDQEERGRITFDDFCGFAEMYPDFAEDYLYPDQTHSDSCAQTPPAPTPNGFCIDFSPEHSDFGRKNSCKKVD |
Proteomic databases
PTM databases
Expression
Tissue specificity
Enriched in alveolar type II cells of lung. Also highly expressed in stomach.
Induction
By FGF7.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-25 | Disordered | ||||
Sequence: MRLRGRGPRAAPSSSSGAGDARRLA | ||||||
Motif | 135-140 | HXXXXD motif | ||||
Sequence: HSSYFD | ||||||
Domain | 379-414 | EF-hand 1 | ||||
Sequence: PVSDALEDMFSLFDESGGGEIDLREYVVALSVVCRP | ||||||
Domain | 451-486 | EF-hand 2 | ||||
Sequence: ISELTVTDLFQAIDQEERGRITFDDFCGFAEMYPDF | ||||||
Motif | 531-534 | Di-lysine motif | ||||
Sequence: KKVD |
Domain
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine.
The di-lysine motif may confer endoplasmic reticulum localization.
Sequence similarities
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length534
- Mass (Da)59,762
- Last updated2006-07-25 v2
- Checksum952C0478DC66738A
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I6A2C9 | A0A8I6A2C9_RAT | Lpcat1 | 509 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AABR03001349 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AABR03001854 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AB244983 EMBL· GenBank· DDBJ | BAE94689.2 EMBL· GenBank· DDBJ | mRNA |