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Q1G9F8 · PURL_LACDA

Function

function

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.

Catalytic activity

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site53
Binding site56ATP (UniProtKB | ChEBI)
Binding site95ATP (UniProtKB | ChEBI)
Binding site97Mg2+ 1 (UniProtKB | ChEBI)
Binding site98-101substrate
Active site99Proton acceptor
Binding site120substrate
Binding site121Mg2+ 2 (UniProtKB | ChEBI)
Binding site244substrate
Binding site274Mg2+ 2 (UniProtKB | ChEBI)
Binding site318-320substrate
Binding site501ATP (UniProtKB | ChEBI)
Binding site538ATP (UniProtKB | ChEBI)
Binding site539Mg2+ 1 (UniProtKB | ChEBI)
Binding site541substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionphosphoribosylformylglycinamidine synthase activity
Biological Process'de novo' IMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoribosylformylglycinamidine synthase subunit PurL
  • EC number
  • Short names
    FGAM synthase
  • Alternative names
    • Formylglycinamide ribonucleotide amidotransferase subunit II
      (FGAR amidotransferase II
      ; FGAR-AT II
      )
    • Glutamine amidotransferase PurL
    • Phosphoribosylformylglycinamidine synthase subunit II

Gene names

    • Name
      purL
    • Ordered locus names
      Ldb1440

Organism names

Accessions

  • Primary accession
    Q1G9F8

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000503131-740Phosphoribosylformylglycinamidine synthase subunit PurL

Interaction

Subunit

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the FGAMS family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    740
  • Mass (Da)
    80,011
  • Last updated
    2006-06-27 v1
  • MD5 Checksum
    BCF6820BF3CF602BA9770D138156290F
MMQEMTPEEIKEKKPYLDWSLSEEEYDYICDKLLHRLPNYTETGLFAAMWSEHCSYKKSKSVLRLFPTKGPRILQGPGEGAGVVDIGDGQAVVFKAESHNHPTAVEPYQGAATGVGGILRDVFSMGARPVATLDSLHFGELDDAHTRWLLNETVAGIGGYGNCMGIPTVGGELTFDDIYKGNPVMNAMSVGLLDVKDMQKGLAQGEGNAVMYVGAKTGRDGIHGATFASHSFDSEHESQRSAVQVGDPFMEKLLLEACLELTQKHADWLVGIQDMGAAGIVSSSSEMASEGKSGMELNLDLVPQRESGMSAYEIMLSESQERMLLCVKKGHEEDVKKIFDFYDLDAVVIGRITSGHNYVLRHHGEVVCDIPVTSLTEDVLEEPSEEKQPQHMIDDAAKPAWEPEITDLAKTYKQMLAQPTIASKGMFTQTYDSMVRTSTVVGPGEDSGVLRVRGTHKGIAMTTDGNGRFIYLDPEIGGKRAVVEAAGNIIASGAEPLAITDCLNFGNPNEPEVFWELHHSVMGIAKACEVLETPVVSGNVSLYNETDGKSIYPTPMVGMVGLVKNLDHLVRDSFQEEGDDLYLVGQTGADYAGSELQKMLTGEIFGSLNDLDLDHIKDYQKRLLAQMEAGHVASAHDLSEGGLAVSLAESSFGHGIGAEVACDLTSAELFSETPGRFLVSVPSEYSAEFAAALAADAVKIGQTAGDSLKLTLKDQAADLPVAELKQIWEEALPCLMKSKD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CR954253
EMBL· GenBank· DDBJ
CAI98241.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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