Q1ERI2 · CITD_MONPU
- ProteinAldehyde dehydrogenase mpl4
- Genempl4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids501 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Aldehyde dehydrogenase; part of the gene cluster that mediates the biosynthesis of the mycotoxin citrinin, a hepato-nephrotoxic compound to humans due to inhibition of respiration complex III (PubMed:17586673, PubMed:19012408, PubMed:19111642, PubMed:27913218, PubMed:28238725).
The pathway begins with the synthesis of a keto-aldehyde intermediate by the citrinin PKS (pksCT) from successive condensations of 4 malonyl-CoA units, presumably with a simple acetyl-CoA starter unit (PubMed:28238725).
Release of the keto-aldehyde intermediate is consistent with the presence of the C-terminal reductive release domain (PubMed:28238725).
Mp11 collaborates with pksCT by catalyzing the hydrolysis of ACP-bound acyl intermediates to free the ACP from stalled intermediates (By similarity).
Mpl2 then catalyzes the oxidation of the C-12 methyl of the ketone intermediate to an alcohol intermediate which is further oxidized by the oxidoreductase mpl7 to produce a bisaldehyde intermediate (PubMed:27913218).
The fourth catalytic step is catalyzed by the mpl4 aldehyde dehydrogenase (PubMed:27913218).
The final transformation is the reduction of C-3 by mpl6 to provide the chemically stable citrinin nucleus (PubMed:27913218).
The pathway begins with the synthesis of a keto-aldehyde intermediate by the citrinin PKS (pksCT) from successive condensations of 4 malonyl-CoA units, presumably with a simple acetyl-CoA starter unit (PubMed:28238725).
Release of the keto-aldehyde intermediate is consistent with the presence of the C-terminal reductive release domain (PubMed:28238725).
Mp11 collaborates with pksCT by catalyzing the hydrolysis of ACP-bound acyl intermediates to free the ACP from stalled intermediates (By similarity).
Mpl2 then catalyzes the oxidation of the C-12 methyl of the ketone intermediate to an alcohol intermediate which is further oxidized by the oxidoreductase mpl7 to produce a bisaldehyde intermediate (PubMed:27913218).
The fourth catalytic step is catalyzed by the mpl4 aldehyde dehydrogenase (PubMed:27913218).
The final transformation is the reduction of C-3 by mpl6 to provide the chemically stable citrinin nucleus (PubMed:27913218).
Catalytic activity
- an aldehyde + H2O + NAD+ = a carboxylate + 2 H+ + NADH
Pathway
Mycotoxin biosynthesis.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | aldehyde dehydrogenase (NAD+) activity | |
Molecular Function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAldehyde dehydrogenase mpl4
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Monascus
Accessions
- Primary accessionQ1ERI2
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000440320 | 1-501 | Aldehyde dehydrogenase mpl4 | |||
Sequence: MAEAAARILEALPAVCEYRWSCKDASKRFTVVNPATGEPITVVQAGNLDTVQGAIQASHRAFESWRWKTRQERSLYLLQAADELQKHSHELAVLLCLENGKPVKDASFDVGFLVQVFRYFGSIVDKLPSEFFDQGSIYSSVIYEPHGVCVGILPFNWPPVHAGGKLAPCLAAGNTMVLKPGEQAPLTLMRIVEILQSVFPADVVQAVPGLGPEIPQALINHPLVKMVSLTGSTASGSQAAQTAAVTLTPTVLELGGKNAFVVFEDADLELVVRDAIDGAFFNKGESCTAASRILVHKDLYPTLVSRLTAAVKKLRTGDGLDETTHIGPVVSRERQQEVLSYIEQGKREGATLAAQGDPPTAGRLSGGFFVPPTLFTDVTADMTIAQREIFGPVVTVGSFETEEEAVKTVNSSQYGLFAGVYSSDFTRAMRVTRKLDVGVVLVNNYFRALLGTPFGGVKDSGYGREHWIGTLREWSRVKNVRFPSGLSPIPAWGGAVDVCKL |
Structure
Sequence
- Sequence statusComplete
- Length501
- Mass (Da)54,135
- Last updated2006-07-11 v1
- Checksum294E2D92F88C25F0