Q1ENB6 · FOLC_ARATH

Function

function

Catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis (PubMed:17289662).
Plays a role in seed stress response and survival (PubMed:17289662, PubMed:21996493).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Inhibited by sulfanilamide.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
30 μMATP

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site209Mg2+ (UniProtKB | ChEBI)
Binding site249(7,8-dihydropterin-6-yl)methyl diphosphate (UniProtKB | ChEBI)
Binding site286(7,8-dihydropterin-6-yl)methyl diphosphate (UniProtKB | ChEBI)
Binding site305(7,8-dihydropterin-6-yl)methyl diphosphate (UniProtKB | ChEBI)
Binding site378(7,8-dihydropterin-6-yl)methyl diphosphate (UniProtKB | ChEBI)
Binding site423(7,8-dihydropterin-6-yl)methyl diphosphate (UniProtKB | ChEBI)
Binding site458-460(7,8-dihydropterin-6-yl)methyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity
Molecular FunctionATP binding
Molecular Functiondihydropteroate synthase activity
Molecular Functionkinase activity
Molecular Functionmetal ion binding
Biological Processfolic acid biosynthetic process
Biological Processresponse to oxidative stress
Biological Processtetrahydrofolate biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Folate synthesis bifunctional protein

Including 2 domains:

  • Recommended name
    6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
  • EC number
  • Short names
    HPPK
  • Alternative names
    • 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
    • 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (PPPK)
  • Recommended name
    Dihydropteroate synthase
  • EC number
  • Short names
    DHPS

Gene names

    • Name
      CytHPPK/DHPS
    • ORF names
      F23O10.22
      , F4N2.15
    • Ordered locus names
      At1g69190

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q1ENB6
  • Secondary accessions
    • Q9LE77

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

No visible phenotype (PubMed:17289662).
Increased sensitivity to oxidative stress (PubMed:21996493).

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 63 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004328671-484Folate synthesis bifunctional protein

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed exclusively in reproductive tissues.

Induction

Up-regulated by slat stress (PubMed:17289662).
Up-regulated by oxidative stress (PubMed:21996493).

Developmental stage

Expressed in early stages of seed development, until the late globular stage of embryo. Not detected at the heart stage of embryo development.

Gene expression databases

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region15-141HPPK
Domain202-470Pterin-binding
Region204-484DHPS

Sequence similarities

In the N-terminal section; belongs to the HPPK family.
In the C-terminal section; belongs to the DHPS family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    484
  • Mass (Da)
    54,110
  • Last updated
    2006-07-11 v1
  • Checksum
    3F9985DBA8655369
MDFTSLETTTFEEVVIALGSNVGNRMNNFKEALRLMKDYGISVTRHSCLYETEPVHVTDQPRFLNAAIRGVTKLKPHELLNVLKKIEKEMGREENGLRYGPRPLDLDILFYGKHKIISDKLIIPHERIWERPFVLAPLVDLLGTEDIDNDKIVAYWHSLSMHSGGIFQAWERLGGESLLGKDGIIQRVIPIGDHLWDFSKKTYVMGILNLTPDSFSDGGKFQSVDTAVSRVRSMISEGVDIIDIGAQSTRPMASRISSQEEIDRLIPVLKVVRGMAEMKGKLISVDTFNSEVALEAIRNGADILNDVSGGSLDENMHKVVADSDVPYMIMHMRGDPCTMQNKENLEYNEICKDVATELYERVREAELSGIPAWRIMIDPGIGFSKGIDHNLDIVMELPKIREEMAKKSIGLSHAPILIGPSRKRFLGDICGRPEASERDAATVACVTAGILKGANIIRVHNVRDNVDAARLCDAMMTKRFKNVD

Sequence caution

The sequence AAF27067.1 differs from that shown. Reason: Erroneous gene model prediction Translation N-terminally extended.
The sequence AAG52504.1 differs from that shown. Reason: Erroneous gene model prediction Translation N-terminally extended.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ866732
EMBL· GenBank· DDBJ
CAI29255.1
EMBL· GenBank· DDBJ
mRNA
AC008262
EMBL· GenBank· DDBJ
AAF27067.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AC018364
EMBL· GenBank· DDBJ
AAG52504.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002684
EMBL· GenBank· DDBJ
AEE34892.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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