Q1ENB6 · FOLC_ARATH
- ProteinFolate synthesis bifunctional protein
- GeneCytHPPK/DHPS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids484 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis (PubMed:17289662).
Plays a role in seed stress response and survival (PubMed:17289662, PubMed:21996493).
Plays a role in seed stress response and survival (PubMed:17289662, PubMed:21996493).
Catalytic activity
- 6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H+
Cofactor
Activity regulation
Inhibited by sulfanilamide.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
30 μM | ATP |
Pathway
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 209 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 249 | (7,8-dihydropterin-6-yl)methyl diphosphate (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 286 | (7,8-dihydropterin-6-yl)methyl diphosphate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 305 | (7,8-dihydropterin-6-yl)methyl diphosphate (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 378 | (7,8-dihydropterin-6-yl)methyl diphosphate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 423 | (7,8-dihydropterin-6-yl)methyl diphosphate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 458-460 | (7,8-dihydropterin-6-yl)methyl diphosphate (UniProtKB | ChEBI) | ||||
Sequence: RVH |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | dihydropteroate synthase activity | |
Molecular Function | kinase activity | |
Molecular Function | metal ion binding | |
Biological Process | folic acid biosynthetic process | |
Biological Process | response to oxidative stress | |
Biological Process | tetrahydrofolate biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFolate synthesis bifunctional protein
Including 2 domains:
- Recommended name6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
- EC number
- Short namesHPPK
- Alternative names
- Recommended nameDihydropteroate synthase
- EC number
- Short namesDHPS
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ1ENB6
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 63 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000432867 | 1-484 | Folate synthesis bifunctional protein | |||
Sequence: MDFTSLETTTFEEVVIALGSNVGNRMNNFKEALRLMKDYGISVTRHSCLYETEPVHVTDQPRFLNAAIRGVTKLKPHELLNVLKKIEKEMGREENGLRYGPRPLDLDILFYGKHKIISDKLIIPHERIWERPFVLAPLVDLLGTEDIDNDKIVAYWHSLSMHSGGIFQAWERLGGESLLGKDGIIQRVIPIGDHLWDFSKKTYVMGILNLTPDSFSDGGKFQSVDTAVSRVRSMISEGVDIIDIGAQSTRPMASRISSQEEIDRLIPVLKVVRGMAEMKGKLISVDTFNSEVALEAIRNGADILNDVSGGSLDENMHKVVADSDVPYMIMHMRGDPCTMQNKENLEYNEICKDVATELYERVREAELSGIPAWRIMIDPGIGFSKGIDHNLDIVMELPKIREEMAKKSIGLSHAPILIGPSRKRFLGDICGRPEASERDAATVACVTAGILKGANIIRVHNVRDNVDAARLCDAMMTKRFKNVD |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed exclusively in reproductive tissues.
Induction
Developmental stage
Expressed in early stages of seed development, until the late globular stage of embryo. Not detected at the heart stage of embryo development.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 15-141 | HPPK | ||||
Sequence: VIALGSNVGNRMNNFKEALRLMKDYGISVTRHSCLYETEPVHVTDQPRFLNAAIRGVTKLKPHELLNVLKKIEKEMGREENGLRYGPRPLDLDILFYGKHKIISDKLIIPHERIWERPFVLAPLVDL | ||||||
Domain | 202-470 | Pterin-binding | ||||
Sequence: TYVMGILNLTPDSFSDGGKFQSVDTAVSRVRSMISEGVDIIDIGAQSTRPMASRISSQEEIDRLIPVLKVVRGMAEMKGKLISVDTFNSEVALEAIRNGADILNDVSGGSLDENMHKVVADSDVPYMIMHMRGDPCTMQNKENLEYNEICKDVATELYERVREAELSGIPAWRIMIDPGIGFSKGIDHNLDIVMELPKIREEMAKKSIGLSHAPILIGPSRKRFLGDICGRPEASERDAATVACVTAGILKGANIIRVHNVRDNVDAAR | ||||||
Region | 204-484 | DHPS | ||||
Sequence: VMGILNLTPDSFSDGGKFQSVDTAVSRVRSMISEGVDIIDIGAQSTRPMASRISSQEEIDRLIPVLKVVRGMAEMKGKLISVDTFNSEVALEAIRNGADILNDVSGGSLDENMHKVVADSDVPYMIMHMRGDPCTMQNKENLEYNEICKDVATELYERVREAELSGIPAWRIMIDPGIGFSKGIDHNLDIVMELPKIREEMAKKSIGLSHAPILIGPSRKRFLGDICGRPEASERDAATVACVTAGILKGANIIRVHNVRDNVDAARLCDAMMTKRFKNVD |
Sequence similarities
In the N-terminal section; belongs to the HPPK family.
In the C-terminal section; belongs to the DHPS family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length484
- Mass (Da)54,110
- Last updated2006-07-11 v1
- Checksum3F9985DBA8655369
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ866732 EMBL· GenBank· DDBJ | CAI29255.1 EMBL· GenBank· DDBJ | mRNA | ||
AC008262 EMBL· GenBank· DDBJ | AAF27067.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AC018364 EMBL· GenBank· DDBJ | AAG52504.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002684 EMBL· GenBank· DDBJ | AEE34892.1 EMBL· GenBank· DDBJ | Genomic DNA |