Q1EG27 · MYO3B_MOUSE
- ProteinMyosin-IIIb
- GeneMyo3b
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1305 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Probable actin-based motor with a protein kinase activity (By similarity).
Required for normal cochlear hair bundle development and hearing. Plays an important role in the early steps of cochlear hair bundle morphogenesis. Influences the number and lengths of stereocilia to be produced and limits the growth of microvilli within the forming auditory hair bundles thereby contributing to the architecture of the hair bundle, including its staircase pattern (PubMed:26754646).
Involved in the elongation of actin in stereocilia tips by transporting the actin regulatory factor ESPN to the plus ends of actin filaments (PubMed:22264607).
Required for normal cochlear hair bundle development and hearing. Plays an important role in the early steps of cochlear hair bundle morphogenesis. Influences the number and lengths of stereocilia to be produced and limits the growth of microvilli within the forming auditory hair bundles thereby contributing to the architecture of the hair bundle, including its staircase pattern (PubMed:26754646).
Involved in the elongation of actin in stereocilia tips by transporting the actin regulatory factor ESPN to the plus ends of actin filaments (PubMed:22264607).
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | filopodium tip | |
Cellular Component | myosin complex | |
Cellular Component | photoreceptor inner segment | |
Cellular Component | photoreceptor outer segment | |
Cellular Component | stereocilium tip | |
Molecular Function | actin binding | |
Molecular Function | ATP binding | |
Molecular Function | microfilament motor activity | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Biological Process | auditory receptor cell stereocilium organization | |
Biological Process | cochlea morphogenesis | |
Biological Process | positive regulation of filopodium assembly | |
Biological Process | regulation of actin filament length | |
Biological Process | sensory perception of sound | |
Biological Process | visual perception |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMyosin-IIIb
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ1EG27
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
MYO3B single knockout mice do not exhibit early hearing impairment whereas mice with a double knockout of MYO3A and MYO3B are profoundly deaf at 1 month of age. Cochlear hair bundles have abnormally long stereocilia and show dynamic shape defects during development.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 70 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000277830 | 1-1305 | Myosin-IIIb | |||
Sequence: MMLGLESLPDPMETWEIIETIGKGTYGKVYKVANKRDGSLAAVKVLDPVSDMDEEIEAEYNILQFLPSHPNVVKFYGMFYKADRCVGGQLWLVLELCNGGSVTELVKGLLRCGKRLDEAVISYILYGALLGLQHLHCHRIIHRDVKGNNILLTTEGGVKLVDFGVSAQLTSTRLRRNTSVGTPFWMAPEVIACEQQYDSSYDARCDVWSLGITAIELGDGDPPLFEMHPVKMLFKIPRNPPPTLLHPDSWCEEFNHFISQCLIKDFEKRPSVTHLLDHPFIKGTQGKVLCLQKQLAKVLQDQKHRNPVAKTRHERMHTGRPHRVEDAGKCCLEDDLVNLEVLDEDTIIYWLQKRYADALIYTYVGDILIALNPFQNLSIYSPQFSRLYHGVKRSSNPPHIFASADNAYQCLVTFSKDQCIVISGESGSGKTESAHLIVQHLTFLGKADNQTLRQKILQVNSLVEAFGNARTAINDNSSRFGKYLEMMFTPTGAVMGARISEYLLEKSRVIQQAAGEKNFHIFYYIYAGLYHQKKLAEFRLPEEKPPRYIAGETERVMQDITSKESYRTQFEAIQHCFKIIGFADKEVHSVYRILAGILNIGSIEFAAISSQHQTDKSEVPNPEALENAACVLCISSEELQEALTSHCVVTRGETIVRANTVDRAEDVRDAMSKALYGRLFSWIVNRINTLLQPDKNICSAEDRMNVGILDIFGFEDFQRNSFEQLCINIANEQIQYYFNQHVFALEQMEYKNEGVDAVLVQYEDNRPLLDMFLQKPLGLLALLDEESRFPQGTDQTLVDKFEDNLRCKFFWRPKGVELCFGIQHYAGPVLYDASGVLEKNRDTLPADVVVVLRTSENKLLQQLFSIPLTKTGNLAQTRAKITASSRSLPPHFSAGRAKVDTLEVIRHPEETTNMKRQTMASYFRYSLMDLLSKMVVGQPHFIRCIKPNDDRKALQFSQDRVLAQLRSTGILETVSIRRQGYSHRIFFEEFVKRYYYLAFRAHQTPPANKESCVAILEKSRLDHWVLGKTKVFLKYYHVEQLNLLLREVMGRVVMLQAYTKGWLGARRYKRAKEKREKGAITIQSAWRGYDARRKLKQRSRRRSESEAHIHTVLQTTPDQKYCPDSGGESNRGHEETSRNCPAEADTDGHPQAQSPPTGCDVTSGHADTAAGYTVAELSVAGTDVSPSLVYHTASAHQRLSPCEDSLKPGSEEGLSQKQRAPRRRCQQPKMLSSPEDTMYYNQLNGTLEYQGSQRKPRKLGQIKVLDGEDQYYKCLSPGACAPEETHSVHPFFFSSSPREDPFAQH |
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 15-281 | Protein kinase | ||||
Sequence: WEIIETIGKGTYGKVYKVANKRDGSLAAVKVLDPVSDMDEEIEAEYNILQFLPSHPNVVKFYGMFYKADRCVGGQLWLVLELCNGGSVTELVKGLLRCGKRLDEAVISYILYGALLGLQHLHCHRIIHRDVKGNNILLTTEGGVKLVDFGVSAQLTSTRLRRNTSVGTPFWMAPEVIACEQQYDSSYDARCDVWSLGITAIELGDGDPPLFEMHPVKMLFKIPRNPPPTLLHPDSWCEEFNHFISQCLIKDFEKRPSVTHLLDHPFI | ||||||
Domain | 331-1046 | Myosin motor | ||||
Sequence: CLEDDLVNLEVLDEDTIIYWLQKRYADALIYTYVGDILIALNPFQNLSIYSPQFSRLYHGVKRSSNPPHIFASADNAYQCLVTFSKDQCIVISGESGSGKTESAHLIVQHLTFLGKADNQTLRQKILQVNSLVEAFGNARTAINDNSSRFGKYLEMMFTPTGAVMGARISEYLLEKSRVIQQAAGEKNFHIFYYIYAGLYHQKKLAEFRLPEEKPPRYIAGETERVMQDITSKESYRTQFEAIQHCFKIIGFADKEVHSVYRILAGILNIGSIEFAAISSQHQTDKSEVPNPEALENAACVLCISSEELQEALTSHCVVTRGETIVRANTVDRAEDVRDAMSKALYGRLFSWIVNRINTLLQPDKNICSAEDRMNVGILDIFGFEDFQRNSFEQLCINIANEQIQYYFNQHVFALEQMEYKNEGVDAVLVQYEDNRPLLDMFLQKPLGLLALLDEESRFPQGTDQTLVDKFEDNLRCKFFWRPKGVELCFGIQHYAGPVLYDASGVLEKNRDTLPADVVVVLRTSENKLLQQLFSIPLTKTGNLAQTRAKITASSRSLPPHFSAGRAKVDTLEVIRHPEETTNMKRQTMASYFRYSLMDLLSKMVVGQPHFIRCIKPNDDRKALQFSQDRVLAQLRSTGILETVSIRRQGYSHRIFFEEFVKRYYYLAFRAHQTPPANKESCVAILEKSRLDHWVLGKTKVFLKYYHVEQLNLLLR | ||||||
Region | 927-949 | Actin-binding | ||||
Sequence: LMDLLSKMVVGQPHFIRCIKPND | ||||||
Domain | 1048-1077 | IQ 1 | ||||
Sequence: VMGRVVMLQAYTKGWLGARRYKRAKEKREK | ||||||
Domain | 1075-1104 | IQ 2 | ||||
Sequence: REKGAITIQSAWRGYDARRKLKQRSRRRSE | ||||||
Region | 1093-1164 | Disordered | ||||
Sequence: RKLKQRSRRRSESEAHIHTVLQTTPDQKYCPDSGGESNRGHEETSRNCPAEADTDGHPQAQSPPTGCDVTSG | ||||||
Compositional bias | 1109-1123 | Polar residues | ||||
Sequence: IHTVLQTTPDQKYCP | ||||||
Compositional bias | 1127-1142 | Basic and acidic residues | ||||
Sequence: GESNRGHEETSRNCPA | ||||||
Region | 1200-1233 | Disordered | ||||
Sequence: SPCEDSLKPGSEEGLSQKQRAPRRRCQQPKMLSS |
Sequence similarities
In the C-terminal section; belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
In the N-terminal section; belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q1EG27-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsMyosin IIIB1
- Length1,305
- Mass (Da)148,099
- Last updated2008-12-16 v2
- Checksum9B8752F2704C7C80
Q1EG27-2
- Name2
- SynonymsMyosin IIIB2
- Differences from canonical
- 898-898: K → KKSPHSVPSYVLNTSPPK
- 1149-1209: Missing
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Features
Showing features for sequence conflict, alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 505 | in Ref. 1; AAX59998/AAX59999 | ||||
Sequence: E → K | ||||||
Alternative sequence | VSP_036028 | 898 | in isoform 2 | |||
Sequence: K → KKSPHSVPSYVLNTSPPK | ||||||
Compositional bias | 1109-1123 | Polar residues | ||||
Sequence: IHTVLQTTPDQKYCP | ||||||
Compositional bias | 1127-1142 | Basic and acidic residues | ||||
Sequence: GESNRGHEETSRNCPA | ||||||
Alternative sequence | VSP_023112 | 1149-1209 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY830392 EMBL· GenBank· DDBJ | AAX59998.2 EMBL· GenBank· DDBJ | mRNA | ||
AY830393 EMBL· GenBank· DDBJ | AAX59999.2 EMBL· GenBank· DDBJ | mRNA | ||
AL845263 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL929164 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |